In situ labeling of apoptotic cell death in the cerebral cortex and thalamus of rats during development

Anaplastic thyroid carcinoma (ATC) is a rare but highly aggressive human malignancy. It is known that disturbances in apoptotic pathways have a great impact on tumor progression and aggressiveness. In this study the apoptosisrelated molecules Bcl-2 (antiapoptotic), Bax (proapoptotic) and survivin (an inhibitor of apoptosis) were analyzed immunohistochemically in thirty archival cases of ATC. In situ apoptotic cell death was analyzed by the TUNEL method. Mean Bcl-2 staining score (calculated from individual scores from 0-3) was low compared to those for Bax and survivin (p<0.05). High expression of survivin was associated with high Bax expression, and was significantly segregated from high Bcl-2 expressing cases (p<0.05). Despite high Bax expression, apoptotic cell death was low in the investigated carcinomas. In addition, the mean apoptotic index in high survivin expressing carcinomas was significantly lower than in low survivin expressing carcinomas (p<0.05). It could be concluded that down-regulation of Bcl-2 is counterbalanced by up-regulation of survivin, which may overcome the effects of high Bax expression, and, at least partly, explain the low apoptosis rate and high biological aggressiveness of ATC.

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Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: biochemical and in situ detection of deoxyribonucleic acid fragmentation in granulosa cells.

Endocrinology ◽

10.1210/endo.134.1.8275940 ◽

1994 ◽

Vol 134 (1) ◽

pp. 245-252 ◽

Cited By ~ 91

Author(s):

H Billig ◽

I Furuta ◽

A J Hsueh

Keyword(s):

Cell Death ◽

Granulosa Cells ◽

Deoxyribonucleic Acid ◽

Apoptotic Cell ◽

Gonadotropin Releasing Hormone ◽

Apoptotic Cell Death ◽

Releasing Hormone ◽

Rat Ovary ◽

In Situ Detection

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Role of the PI3K/AKT signalling pathway in apoptotic cell death in the cerebral cortex of streptozotocin-induced diabetic rats

Experimental and Therapeutic Medicine ◽

10.3892/etm.2017.4259 ◽

2017 ◽

Vol 13 (5) ◽

pp. 2417-2422 ◽

Cited By ~ 14

Author(s):

Yan Meng ◽

Weiwei Wang ◽

Jinsong Kang ◽

Xinxue Wang ◽

Liankun Sun

Keyword(s):

Cell Death ◽

Cerebral Cortex ◽

Apoptotic Cell ◽

Diabetic Rats ◽

Apoptotic Cell Death ◽

Signalling Pathway

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Characterization and Quantification of Isoflurane-Induced Developmental Apoptotic Cell Death in Mouse Cerebral Cortex

Anesthesia & Analgesia ◽

10.1213/ane.0b013e318281e988 ◽

2013 ◽

Vol 116 (4) ◽

pp. 845-854 ◽

Cited By ~ 51

Author(s):

George K. Istaphanous ◽

Christopher G. Ward ◽

Xinyu Nan ◽

Elizabeth A. Hughes ◽

John C. McCann ◽

...

Keyword(s):

Cell Death ◽

Cerebral Cortex ◽

Apoptotic Cell ◽

Apoptotic Cell Death ◽

Mouse Cerebral Cortex

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Apoptotic cell death in the porcine endometrium during the oestrous cycle

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.1.9 ◽

2001 ◽

Vol 49 (1) ◽

pp. 71-79 ◽

Cited By ~ 8

Author(s):

Barbara Wąsowska ◽

Beata Ludkiewicz ◽

Stanisława Stefańczyk-Krzymowska ◽

W. Grzegorzewski ◽

Janina Skipor

Keyword(s):

Cell Death ◽

Stromal Cells ◽

Cell Kinetics ◽

Apoptotic Cell ◽

Apoptotic Cell Death ◽

Oestrous Cycle ◽

Apoptotic Cells ◽

Early Follicular Phase ◽

Labelling Method

It has been reported that apoptosis plays an essential role in controlling the physiological cell kinetics in the human and rodent endometrium but this type of death has never been studied in the porcine endometrium. The aim of this study was to investigate the apoptotic cell death in the porcine endometrium during the middle (Days 9–11) and late (Day 13) luteal phase, during the luteolysis (Day 15) and early follicular phase (Days 17–19) of the oestrous cycle. Apoptotic cells were identified by in situ DNA 3′-end labelling method. it was revealed that the greatest number of apoptotic cells in the luminal and glandular epithelium was found on Days 17–19 and on Day 15 of the oestrous cycle, respectively. in the stroma, the greatest number of these cells was found on Days 9–11. Our data have shown that in the porcine endometrium, both epithelial and stromal cells undergo apoptosis and that the number of apoptotic cells varies depending on the phase of the oestrous cycle.

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