scholarly journals Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome

2020 ◽  
Vol 59 (1) ◽  
Author(s):  
Kevin Chiem ◽  
Chengjin Ye ◽  
Luis Martinez‐Sobrido
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome

scholarly journals Targeting CreERT2 expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis

2012 ◽  
Vol 21 (5) ◽  
pp. 1117-1123 ◽  
Author(s):  
Li Zhang ◽  
Boyu Zhang ◽  
Sang Jun Han ◽  
Amy N. Shore ◽  
Jeffrey M. Rosen ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Keratin 8

scholarly journals Revising the Chromosome-Specific Probes of White Hawk (Leucopternis albicollis)

Proceedings ◽  
2020 ◽  
Vol 76 (1) ◽  
pp. 1
Author(s):  
Ivanete Furo ◽  
Rafael Kretschmer ◽  
Jorge Pereira ◽  
Darren Griffin ◽  
Rebecca O’Connor ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Bird Of Prey ◽  
Inaccurate Data ◽  
Intrachromosomal Rearrangements

Leucopternis albicollis is a diurnal bird of prey with extensive karyotype reorganization. Chromosome-specific probes from this species have been used successfully to detect intrachromosomal rearrangements in different species of bird since 2010. However, some gaps were detected in this first set of probes. Here, we have obtained a new set of whole chromosome probes in order to improve the previous one; also, we have performed experiments using bacterial artificial chromosome (BAC) from chicken microchromosomes. Our results demonstrated that the microchromosomes were involved in fusion events. In addition, a new nomenclature has been proposed for the new set of probes and some inaccurate data were corrected.

Cloning of a Very Virulent Plus, 686 Strain of Marek's Disease Virus as a Bacterial Artificial Chromosome

2013 ◽  
Vol 57 (2s1) ◽  
pp. 469-473 ◽  
Author(s):  
Sanjay M. Reddy ◽  
Aijun Sun ◽  
Owais A. Khan ◽  
Lucy F. Lee ◽  
Blanca Lupiani
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Disease Virus ◽  
Artificial Chromosome ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease

Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla)

2008 ◽  
Vol 122 (1) ◽  
pp. 55-60 ◽  
Author(s):  
J. Che ◽  
J. Wang ◽  
W. Su ◽  
J. Ye ◽  
Y. Wang ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome ◽  
Fish Mapping ◽  
Manis Pentadactyla

Construction and characterization of a bacterial artificial chromosome library for Camellia sinensis

2017 ◽  
Vol 13 (4) ◽  
Author(s):  
Yuling Tai ◽  
Hao Wang ◽  
Chaoling Wei ◽  
Ling Su ◽  
Min Li ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Camellia Sinensis ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome

Construction and initial analysis of bacterial artificial chromosome (BAC) contigs from the sex-determining region of the platyfish Xiphophorus maculatus

Gene ◽  
2002 ◽  
Vol 295 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Alexander Froschauer ◽  
Cornelia Körting ◽  
Takayuki Katagiri ◽  
Takashi Aoki ◽  
Shuichi Asakawa ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Initial Analysis ◽  
Xiphophorus Maculatus ◽  
Bac Contigs

Herpes Simplex Virus Type 1 DNA Amplified as Bacterial Artificial Chromosome inEscherichia coli:Rescue of Replication-Competent Virus Progeny and Packaging of Amplicon Vectors

1998 ◽  
Vol 9 (18) ◽  
pp. 2787-2794 ◽  
Author(s):  
Yoshinaga Saeki ◽  
Tomotsugu Ichikawa ◽  
Akane Saeki ◽  
E. Antonio Chiocca ◽  
Kurt Tobler ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Bacterial Artificial Chromosome ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Artificial Chromosome ◽  
Replication Competent Virus ◽  
Virus Progeny ◽  
Simplex Virus

The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis

Blood ◽  
2003 ◽  
Vol 102 (8) ◽  
pp. 2856-2861 ◽  
Author(s):  
Hongmin Sun ◽  
Tony L. Yang ◽  
Angela Yang ◽  
Xixi Wang ◽  
David Ginsburg
Keyword(s):  
Gene Expression ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Coagulation Factor ◽  
Artificial Chromosome ◽  
Coagulation Cascade ◽  
Factor V ◽  
Wild Type ◽  
Plasma Factor ◽  
Coagulation Factor V

Abstract Coagulation factor V (FV) is a central regulator of the coagulation cascade. Circulating FV is found in plasma and within platelet α granules. The specific functions of these distinct FV pools are uncertain. We now report the generation of transgenic mice with FV gene expression restricted to either the liver or megakaryocyte/platelet lineage using bacterial artificial chromosome (BAC) constructs. Six of 6 independent albumin BAC transgenes rescue the neonatal lethal hemorrhage of FV deficiency. Rescued mice all exhibit liver-specific Fv expression at levels ranging from 6% to 46% of the endogenous Fv gene, with no detectable FV activity within the platelet pool. One of the 3 Pf4 BAC transgenes available for analysis also rescues the lethal FV null phenotype, with FV activity restricted to only the platelet pool (approximately 3% of the wild-type FV level). FV-null mice rescued by either the albumin or Pf4 BAC exhibit nearly normal tail bleeding times. These results demonstrate that Fv expression in either the platelet or plasma FV pool is sufficient for basal hemostasis. In addition, these findings indicate that the murine platelet and plasma FV pools are biosynthetically distinct, in contrast to a previous report demonstrating a plasma origin for platelet FV in humans.

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