Lymphoreticular malignancies in serous effusions: Cytomorphologic, flow cytometric and immunocytochemical analysis

2021 ◽  
Vol 49 (5) ◽  
pp. 647-656
Author(s):  
Parikshaa Gupta ◽  
Tushar Pandey ◽  
Upasana Gautam ◽  
Arvind Rajwanshi ◽  
Radhika Srinivasan ◽  
...  
Keyword(s):  
Immunocytochemical Analysis ◽  
Flow Cytometric

Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5′-bromo-2′-deoxyuridine

2005 ◽  
Vol 289 (3) ◽  
pp. C757-C765 ◽  
Author(s):  
Chih-Horng Shih ◽  
Beth A. Whalen ◽  
Yukinobu Goto ◽  
James C. Hogg ◽  
Stephan F. van Eeden
Keyword(s):  
Bone Marrow ◽  
Tissue Injury ◽  
Accurate Method ◽  
Immunocytochemical Analysis ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Transit Times ◽  
Brdu Labeling ◽  
Time Calculation ◽  
And Behavior

Inflammation accelerates polymorphonuclear leukocyte (PMN) release from the bone marrow, and these PMNs are implicated in inappropriate tissue injury. We have previously developed a method using 5′-bromo-2′-deoxyuridine (BrdU) to study PMN kinetics using an immunocytochemical grading system of PMN on cytospin slides. The aim of this study was to develop a flow cytometric method to quantify the number of positively stained PMN and grade the intensity of staining for the transit time calculation of PMN through the marrow. Dividing myeloid progenitors in the marrow of rabbits were labeled with a pulse dosage of intravenous BrdU. BrdU-labeled PMN (PMNBrdU) were detected in the circulation using a FITC-conjugated anti-BrdU monoclonal antibody. The PMNBrdUwere assigned to five groups according to their FITC intensity, and the transit times of PMN at different stages of development in the marrow were calculated. Results were compared using parallel immunocytochemical analysis of the same samples. In control animals, PMNBrdUin the circulation peaked at 72 h after BrdU labeling with 36.0% of PMN labeled. In normal rabbits, the transit times of PMN through the mitotic pool (49.5 ± 4.2 h) and maturation pool (65.5 ± 3.1 h) correlated well with immunocytochemical analysis and previously published values. Using this method, we demonstrated that exposure to air pollution particles accelerates the release of PMNBrdUfrom the marrow. We conclude that a flow cytometric approach for identifying BrdU-labeled leukocytes provides an objective and accurate method for studying leukocyte kinetics and behavior.

Detection of tumor cells in body cavity fluids by flow cytometric and immunocytochemical analysis

2006 ◽  
Vol 34 (8) ◽  
pp. 528-541 ◽  
Author(s):  
Awtar Krishan ◽  
Parvin Ganjei-Azar ◽  
Merce Jorda ◽  
Ronald M. Hamelik ◽  
Isildinha M. Reis ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Body Cavity ◽  
Immunocytochemical Analysis ◽  
Flow Cytometric

IVBT-documented platelet function correlates with flow cytometric data

1996 ◽  
Vol 17 (4) ◽  
pp. 553-558 ◽  
Author(s):  
J HOFFMANN
Keyword(s):  
Platelet Function ◽  
Flow Cytometric Data ◽  
Flow Cytometric

Re: Lymph node sampling for flow cytometric analysis

Pathology ◽  
2003 ◽  
Vol 35 (2) ◽  
pp. 183-183
Author(s):  
Ibrahim Zardawi
Keyword(s):  
Lymph Node ◽  
Flow Cytometric Analysis ◽  
Lymph Node Sampling ◽  
Flow Cytometric

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells

Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

1994 ◽  
Vol 72 (05) ◽  
pp. 762-769 ◽  
Author(s):  
Toshiro Takafuta ◽  
Kingo Fujirmura ◽  
Hironori Kawano ◽  
Masaaki Noda ◽  
Tetsuro Fujimoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Specific Protein ◽  
Platelet Membrane ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polymerase Chain ◽  
Megakaryocytic Cell

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

Platelet Activation and Cytosolic Free Potassium Kinetics: A Dynamic Flow Cytometric Monitoring

2019 ◽  
Author(s):  
A. Aliotta ◽  
D. Bertaggia ◽  
L. Alberio
Keyword(s):  
Platelet Activation ◽  
Dynamic Flow ◽  
Flow Cytometric

Flow Cytometric Quantification of Human Platelet Membrane Glycoproteins in Citrated Whole Blood

2019 ◽  
Author(s):  
C. Berens ◽  
H. Rühl ◽  
J. Müller ◽  
J. Oldenburg ◽  
B. Pötzsch
Keyword(s):  
Whole Blood ◽  
Human Platelet ◽  
Platelet Membrane ◽  
Membrane Glycoproteins ◽  
Flow Cytometric
Sign in / Sign up

Export Citation Format

Share Document