scholarly journals A nontoxic fungal natural product modulates fin regeneration in zebrafish larvae upstream of FGF‐WNT developmental signaling

2020 ◽  
Author(s):  
Paul Cavanah ◽  
Junji Itou ◽  
Yudi Rusman ◽  
Naoyuki Tahara ◽  
Jessica M. Williams ◽  
...  
Keyword(s):  
Natural Product ◽  
Fin Regeneration ◽  
Zebrafish Larvae ◽  
Developmental Signaling

scholarly journals Early redox, Src family kinase, and calcium signaling integrate wound responses and tissue regeneration in zebrafish

2012 ◽  
Vol 199 (2) ◽  
pp. 225-234 ◽  
Author(s):  
Sa Kan Yoo ◽  
Christina M. Freisinger ◽  
Danny C. LeBert ◽  
Anna Huttenlocher
Keyword(s):  
Calcium Signaling ◽  
Tissue Regeneration ◽  
Tissue Injury ◽  
Src Family Kinase ◽  
Fin Regeneration ◽  
Epimorphic Regeneration ◽  
Zebrafish Larvae ◽  
Redox Sensor ◽  
Wound Responses ◽  
Early Wound

Tissue injury can lead to scar formation or tissue regeneration. How regenerative animals sense initial tissue injury and transform wound signals into regenerative growth is an unresolved question. Previously, we found that the Src family kinase (SFK) Lyn functions as a redox sensor in leukocytes that detects H2O2 at wounds in zebrafish larvae. In this paper, using zebrafish larval tail fins as a model, we find that wounding rapidly activated SFK and calcium signaling in epithelia. The immediate SFK and calcium signaling in epithelia was important for late epimorphic regeneration of amputated fins. Wound-induced activation of SFKs in epithelia was dependent on injury-generated H2O2. A SFK member, Fynb, was responsible for fin regeneration. This work provides a new link between early wound responses and late regeneration and suggests that redox, SFK, and calcium signaling are immediate “wound signals” that integrate early wound responses and late epimorphic regeneration.

scholarly journals Molecular and cellular determinants of motor asymmetry in zebrafish

2019 ◽  
Author(s):  
Eric J. Horstick ◽  
Yared Bayleyen ◽  
Harold A. Burgess
Keyword(s):  
Visual Information ◽  
Motor Behavior ◽  
Hand Preference ◽  
Neural Substrates ◽  
Developmental Pathways ◽  
Human Hand ◽  
Motor Asymmetry ◽  
Zebrafish Larvae ◽  
Developmental Signaling ◽  
Functional Lateralization

AbstractAsymmetries in motor behavior, such as human hand preference, are observed throughout bilateria. However, neural substrates and developmental signaling pathways that impose underlying functional lateralization on a broadly symmetric nervous system are unknown. Here we report that in the absence of over-riding visual information, zebrafish larvae show intrinsic lateralized motor behavior that is mediated by a cluster of 60 posterior tuberculum (PT) neurons in the forebrain. PT neurons impose motor bias via a projection through the epithalamic commissure to the habenula. Acquisition of left/right identity is disrupted by heterozygous mutations in mosaic eyes and mindbomb, genes that regulate Notch signaling. These results define the neuronal substrate for motor asymmetry in a vertebrate and support the idea that developmental pathways that establish visceral asymmetries also govern acquisition of left/right identity.

Inhibitory effects of polystyrene microplastics on caudal fin regeneration in zebrafish larvae

2020 ◽  
Vol 266 ◽  
pp. 114664
Author(s):  
Linqi Gu ◽  
Li Tian ◽  
Gan Gao ◽  
Shaohong Peng ◽  
Jieyu Zhang ◽  
...  
Keyword(s):  
Inhibitory Effects ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Zebrafish Larvae

scholarly journals NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Béryl Laplace-Builhé ◽  
Audrey Barthelaix ◽  
Said Assou ◽  
Candice Bohaud ◽  
Marine Pratlong ◽  
...  
Keyword(s):  
Neural Crest ◽  
Transgenic Zebrafish ◽  
Critical Function ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Blastema Formation ◽  
Zebrafish Larvae ◽  
Single Cell Rna Sequencing ◽  
Chemical Inhibition ◽  
Macrophage Subsets

AbstractFish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.

scholarly journals Correction to: TNF signaling and macrophages govern fin regeneration in zebrafish larvae

2018 ◽  
Vol 9 (6) ◽  
Author(s):  
Mai Nguyen-Chi ◽  
Béryl Laplace-Builhé ◽  
Jana Travnickova ◽  
Patricia Luz-Crawford ◽  
Gautier Tejedor ◽  
...  
Keyword(s):  
Fin Regeneration ◽  
Zebrafish Larvae

Effects of 17α‑ethinylestradiol on caudal fin regeneration in zebrafish larvae

2019 ◽  
Vol 653 ◽  
pp. 10-22 ◽  
Author(s):  
Liwei Sun ◽  
Linqi Gu ◽  
Hana Tan ◽  
Pan Liu ◽  
Gan Gao ◽  
...  
Keyword(s):  
Caudal Fin ◽  
Fin Regeneration ◽  
Zebrafish Larvae

scholarly journals TNF signaling and macrophages govern fin regeneration in zebrafish larvae

2017 ◽  
Vol 8 (8) ◽  
pp. e2979-e2979 ◽  
Author(s):  
Mai Nguyen-Chi ◽  
Béryl Laplace-Builhé ◽  
Jana Travnickova ◽  
Patricia Luz-Crawford ◽  
Gautier Tejedor ◽  
...  
Keyword(s):  
Cell Activation ◽  
Molecular Mechanisms ◽  
Blastema Cell ◽  
Direct Role ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Blastema Formation ◽  
Zebrafish Larvae ◽  
Macrophage Subset ◽  
Inflammatory Macrophages

Abstract Macrophages are essential for appendage regeneration after amputation in regenerative species. The molecular mechanisms through which macrophages orchestrate blastema formation and regeneration are still unclear. Here, we use the genetically tractable and transparent zebrafish larvae to study the functions of polarized macrophage subsets during caudal fin regeneration. After caudal fin amputation, we show an early and transient accumulation of pro-inflammatory macrophages concomitant with the accumulation of non-inflammatory macrophages which, in contrast to pro-inflammatory macrophages, remain associated to the fin until the end of the regeneration. Chemical and genetic depletion of macrophages suggested that early recruited macrophages that express TNFα are critical for blastema formation. Combining parabiosis and morpholino knockdown strategies, we show that TNFα/TNFR1 signaling pathway is required for the fin regeneration. Our study reveals that TNFR1 has a necessary and direct role in blastema cell activation suggesting that macrophage subset balance provides the accurate TNFα signal to prime regeneration in zebrafish.

Synthetic studies toward inducamide C

2021 ◽  
Author(s):  
Ardalan A. Nabi ◽  
Lydia M. Scott ◽  
Daniel P. Furkert ◽  
Jonathan Sperry
Keyword(s):  
Natural Product ◽  
Structural Revision ◽  
Synthetic Studies

The rare benzoxazepine ring in the alkaloid inducamide C is unstable and prone to rearrangement, indicating that structural revision of the natural product may be necessary.

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