Use of two gRNAs for CRISPR/Cas9 improves bi-allelic homologous recombination efficiency in mouse embryonic stem cells

genesis ◽

10.1002/dvg.23212 ◽

2018 ◽

Vol 56 (5) ◽

pp. e23212 ◽

Author(s):

Sandra Acosta ◽

Luciano Fiore ◽

Isabel Anna Carota ◽

Guillermo Oliver

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Recombination Efficiency

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Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.87.24.9918 ◽

1990 ◽

Vol 87 (24) ◽

pp. 9918-9922 ◽

Author(s):

T. Yagi ◽

Y. Ikawa ◽

K. Yoshida ◽

Y. Shigetani ◽

N. Takeda ◽

...

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Diphtheria Toxin ◽

Negative Selection ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Toxin A ◽

Diphtheria Toxin A

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Gene Targeting of the Receptor-Like Protein Tyrosine Phosphatase Lar by Homologous Recombination in Mouse Embryonic Stem Cells

Signalling Mechanisms — from Transcription Factors to Oxidative Stress ◽

10.1007/978-3-642-79675-3_29 ◽

1995 ◽

pp. 407-419

Author(s):

Roel Schaapveld ◽

Jan Schepens ◽

Frank Oerlemans ◽

Michel Streuli ◽

Bé Wieringa ◽

...

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Gene Targeting ◽

Protein Tyrosine Phosphatase ◽

Tyrosine Phosphatase ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Protein Tyrosine

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Approaching the multifaceted nature of energy metabolism: inactivation of the cytosolic creatine kinases via homologous recombination in mouse embryonic stem cells

Molecular and Cellular Biochemistry ◽

10.1007/bf01267959 ◽

1994 ◽

Vol 133-134 (1) ◽

pp. 263-274 ◽

Author(s):

J. van Deursen ◽

B. Wieringa

Keyword(s):

Embryonic Stem Cells ◽

Energy Metabolism ◽

Homologous Recombination ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells

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Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection

Methods in Molecular Biology - Microinjection ◽

10.1007/978-1-4939-8831-0_7 ◽

2018 ◽

pp. 115-137 ◽

Author(s):

Pinghu Liu ◽

Yan Li ◽

Jingqi Lei ◽

Lijin Dong

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

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Gene Replacement of the p53 Gene with the lacZ Gene in Mouse Embryonic Stem-Cells and Mice by Using Two Steps of Homologous Recombination

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1994.2005 ◽

1994 ◽

Vol 202 (2) ◽

pp. 830-837 ◽

Author(s):

Y. Gondo ◽

K. Nakamura ◽

K. Nakao ◽

T. Sasaoka ◽

K. Ito ◽

...

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Embryonic Stem ◽

P53 Gene ◽

Gene Replacement ◽

Mouse Embryonic Stem Cells ◽

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Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system

Nucleic Acids Research ◽

10.1093/nar/19.25.7171 ◽

1991 ◽

Vol 19 (25) ◽

pp. 7171-7175 ◽

Author(s):

Maria Jasin ◽

Feng Liang

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Embryonic Stem ◽

Chloramphenicol Acetyltransferase ◽

Mouse Embryonic Stem Cells ◽

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Ataxia Telangiectasia Mutated (ATM) Is Dispensable for Endonuclease I-SceI-induced Homologous Recombination in Mouse Embryonic Stem Cells

Journal of Biological Chemistry ◽

10.1074/jbc.m112.445825 ◽

2013 ◽

Vol 288 (10) ◽

pp. 7086-7095 ◽

Author(s):

Emilie Rass ◽

Gurushankar Chandramouly ◽

Shan Zha ◽

Frederick W. Alt ◽

Anyong Xie

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Ataxia Telangiectasia ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Ataxia Telangiectasia Mutated

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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Journal of Visualized Experiments ◽

10.3791/58467 ◽

2018 ◽

Author(s):

Dan Zhou ◽

Lei Tan ◽

Jian Li ◽

Tanbin Liu ◽

Yi Hu ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Embryonic Stem Cells ◽

Homologous Recombination ◽

Southern Blotting ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Chain Reaction ◽

Polymerase Chain

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Novel vectors for homologous recombination strategies in mouse embryonic stem cells: An ES cell line expressing EGFP under control of the 5T4 promoter

Experimental Cell Research ◽

10.1016/j.yexcr.2007.07.021 ◽

2007 ◽

Vol 313 (16) ◽

pp. 3604-3615 ◽

Author(s):

Flor M. Perez-Campo ◽

Helen L. Spencer ◽

Rhoderick H. Elder ◽

Peter L. Stern ◽

Christopher M. Ward

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Cell Line ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

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Inactivating the beta 2-microglobulin locus in mouse embryonic stem cells by homologous recombination.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.86.22.8932 ◽

1989 ◽

Vol 86 (22) ◽

pp. 8932-8935 ◽

Author(s):

B. H. Koller ◽

O. Smithies

Keyword(s):

Embryonic Stem Cells ◽

Homologous Recombination ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Beta 2 ◽

Beta 2 Microglobulin

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