<p>In grassland ecosystems nitrogen (N) inputs are mainly attributed to fertilizer applications for increasing&#160; herbage productivity and to excreta of grazing animals. Cattle, for instance, excrete 75-95 % of the N intake. Accordingly, dung and urine patches of grazing animals form hotspots of nitrate leaching and gaseous N emissions as ammonia (NH<sub>3</sub>) or the important greenhouse gas nitrous oxide (N<sub>2</sub>O). Global default emission factor (EF) values for N<sub>2</sub>O, 2.0 % for grazing based nitrogen inputs (EF3) and 1.0 % for nitrogen inputs via fertilizer applications (EF1) have been suggested by IPCC. However, some countries like New Zealand, Canada or the Netherlands have established country-specific EFs showing considerable regional differences.</p><p>In the present research study, we examine N<sub>2</sub>O emissions of a pasture field in Switzerland in relation to possible drivers. Field scale emissions by eddy covariance are measured in parallel to patch-scale N<sub>2</sub>O fluxes from&#160;controlled applications of urine, dung and fertilizer. The patch-scale fluxes are measured by a manually&#160;operated chamber ('fast-box') connected to an online gas analyzer. Besides estimating EF values on annual&#160;and seasonal basis, relevant factors that might control N<sub>2</sub>O fluxes like environmental conditions (weather parameters, soil moisture, soil temperature), vegetation characteristics (height, composition, nitrogen and&#160;carbon content) and pasture management (patch age, grazing, fertilization, cut events, interactive effects) are analyzed.&#160;</p><p>We present and discuss results of the first measurement year 2020. Three artificial urine applications during summer and autumn were performed. They show peak N<sub>2</sub>O fluxes of 279-1718 &#956;g m<sup>-2</sup> h<sup>-1</sup> directly after application that decrease to near-background fluxes within 19-43 days. Using a simple linear interpolation of measured N<sub>2</sub>O fluxes, EF values of artificial urine patches vary between 0.57 and 2.44 % indicating a seasonal variability of N<sub>2</sub>O fluxes.</p>