scholarly journals Pollen DNA metabarcoding identifies regional provenance and high plant diversity in Australian honey

2021 ◽  
Author(s):  
Liz Milla ◽  
Kale Sniderman ◽  
Rose Lines ◽  
Mahsa Mousavi‐Derazmahalleh ◽  
Francisco Encinas‐Viso
Keyword(s):  
Plant Diversity ◽  
Dna Metabarcoding

Estimating aquatic plant diversity and distribution in rivers from Jingjinji Region, China, using environmental DNA metabarcoding and a traditional survey method

2021 ◽  
pp. 111348
Author(s):  
Fenfen Ji ◽  
Liang Yan ◽  
Saihong Yan ◽  
Tianlong Qin ◽  
Jianzhong Shen ◽  
...  
Keyword(s):  
Plant Diversity ◽  
Aquatic Plant ◽  
Environmental Dna ◽  
Survey Method ◽  
Dna Metabarcoding

Metabarcoding of modern sedimentary DNA from the Tibetan Plateau and Siberia as a training dataset for vegetation reconstructions

2021 ◽  
Author(s):  
Weihan Jia ◽  
Kathleen Stoof-Leichsenring ◽  
Sisi Liu ◽  
Kai Li ◽  
Sichao Huang ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Lake Sediments ◽  
Plant Diversity ◽  
Lake Water ◽  
Aquatic Plant ◽  
Freshwater Ecosystems ◽  
The Tibetan Plateau ◽  
Plant Dna ◽  
Continental Scale ◽  
Dna Metabarcoding

<p>Lake sedimentary DNA (<em>sed</em>DNA) is an established tool to trace past changes in vegetation composition and plant diversity. However, little is known about the relationships between sedimentary plant DNA and modern vegetational and environmental conditions. In this study, we investigate i) the relationships between the preservation of sedimentary plant DNA and environmental variables, ii) the modern analogue of ancient plant DNA assemblages archived in lake sediments, and iii) the usability of sedimentary plant DNA for characterization of terrestrial and aquatic plant composition and diversity based on a large dataset of PCR-amplified plant DNA data retrieved from 259 lake surface sediments from the Tibetan Plateau and Siberia. Our results indicate the following: i) Lake-water electrical conductivity and pH are the most important variables for the preservation of plant DNA in lake sediments. We expect the best preservation conditions for sedimentary plant DNA in small deep lakes characterized by high water conductivities (≥100 μS cm<sup>-1</sup>) and neutral to slightly alkaline pH conditions (7–9). ii) Plant DNA metabarcoding is promising for palaeovegetation reconstruction in high mountain regions, where shifts in vegetation are solely captured by the <em>sed</em>DNA-based analogue matching and fossil pollen generally has poor modern analogues. However, the biases in the representation of some taxa could lead to poor analogue conditions. iii) Plant DNA metabarcoding is a reliable proxy to reflect modern vegetation types and climate characteristics at a sub-continental scale. However, the resolution of the <em>trn</em>L P6 loop marker, the incompleteness of the reference library, and the extent of <em>sed</em>DNA preservation are still the main limitations of this method. iv) Plant DNA metabarcoding is a suitable proxy to recover modern aquatic plant diversity, which is mostly affected by July temperature and lake-water conductivity. Ongoing warming might decrease macrophyte richness in the Tibetan Plateau and Siberia, and ultimately threaten the health of these important freshwater ecosystems. To conclude, sedimentary plant DNA presents a high correlation with modern vegetation and may therefore be an important proxy for reconstruction of past vegetation.</p>

scholarly journals Unravelling the plant diversity of the Amazonian canga through DNA barcoding

2021 ◽  
Author(s):  
Santelmo Vasconcelos ◽  
Gisele Nunes ◽  
Mariana Dias ◽  
Jamily Lorena ◽  
Renato Oliveira ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Plant Diversity ◽  
Vascular Plants ◽  
Dna Barcode ◽  
Brazilian Amazon ◽  
Dna Barcodes ◽  
Dna Metabarcoding ◽  
Industrial Mining ◽  
First Time ◽  
Species Being

The canga of the Serra dos Carajás, in Eastern Amazon, is home to a unique open plant community, harbouring several endemic and rare species. Although a complete flora survey has been recently published, scarce to no genetic information is available for most plant species of the ironstone outcrops of the Serra dos Carajás. In this scenario, DNA barcoding appears as a fast and effective approach to assess the genetic diversity of the Serra dos Carajás flora, considering the growing need for robust biodiversity conservation planning in such an area with industrial mining activities. Thus, after testing eight different DNA barcode markers (matK, rbcL, rpoB, rpoC1, atpF-atpH, psbK-psbI, trnH-psbA and ITS2), we chose rbcL and ITS2 as the most suitable markers for a broad application in the regional flora. Here we describe DNA barcodes for 1,130 specimens of 538 species, 323 genera and 115 families of vascular plants, with a total of 344 species being barcoded for the first time. In addition, we assessed the potential of using DNA metabarcoding of bulk samples for surveying plant diversity in the canga. Upon achieving the first comprehensive DNA barcoding effort directed to a complete flora in the Brazilian Amazon, we discuss the relevance of our results to guide future conservation measures in the Serra dos Carajás.

scholarly journals Evaluating the efficacy of sample collection approaches and DNA metabarcoding for identifying the diversity of plants utilized by nectivorous bats

Genome ◽  
2019 ◽  
Vol 62 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Christine E. Edwards ◽  
Joel F. Swift ◽  
Richard F. Lance ◽  
Thomas A. Minckley ◽  
Denise L. Lindsay
Keyword(s):  
Plant Species ◽  
Plant Diversity ◽  
Species Interactions ◽  
Careful Analysis ◽  
Food Plants ◽  
Sample Collection ◽  
Visual Identification ◽  
Dna Metabarcoding ◽  
By Catch ◽  
Main Food Source

In this study, we evaluated the efficacy of sample collection approaches and DNA metabarcoding to identify plants utilized by nectivorous bats. Samples included guano collected from beneath bat roosts and pollen-swabs from bat fur, both of which were subjected to DNA metabarcoding and visual identification of pollen (microscopy) to measure plant diversity. Our objectives were to determine whether DNA metabarcoding could detect likely food plants of nectivorous bats, whether sample types would produce different estimates of plant diversity, and to compare results of DNA metabarcoding to visual identification. Visual identification found that 99% of pollen was from Agave, which is thought to be the bats’ main food source. The dominant taxon found by metabarcoding was also Agavoideae, but a broader diversity of plant species was also detected, many of which are likely “by-catch” from the broader environment. Metabarcoding outcomes differed between sample types, likely because pollen-swabs measured the plant species visited by bats and guano samples measured all items consumed in the bat’s diet, even those that were not pollen or nectar. Overall, metabarcoding is a powerful, high-throughput tool to understand bat ecology and species interactions, but careful analysis of results is necessary to derive accurate ecological conclusions.

Plant diversity and species richness of Ljubljana marsh grasslands under the influence of different cutting and fertilizing regimes

2009 ◽  
Vol 57 (2) ◽  
pp. 197-203 ◽  
Author(s):  
T. Sinkovč
Keyword(s):  
Species Richness ◽  
Plant Diversity ◽  
Grassland Management ◽  
Botanical Composition ◽  
Significant Negative Effect ◽  
Negative Effect ◽  
Cutting Regimes ◽  
N Rates ◽  
Exploitation Intensity ◽  
Plot Design

The botanical composition of grasslands determines the agronomic and natural values of swards. Good grassland management usually improves herbage value, but on the other hand it frequently decreases the plant diversity and species richness in the swards. In 1999 a field trial in a split-plot design with four replicates was therefore established on the Arrhenatherion type of vegetation in Ljubljana marsh meadows in order to investigate this relationship. Cutting regimes (2 cuts — with normal and delayed first cut, 3 cuts and 4 cuts per year) were allocated to the main plots and fertiliser treatments (zero fertiliser — control, PK and NPK with 2 or 3 N rates) were allocated to the sub-plots. The results at the 1 st cutting in the 5 th trial year were as follows: Fertilising either with PK or NPK had no significant negative effect on plant diversity in any of the cutting regimes. In most treatments the plant number even increased slightly compared to the control. On average, 20 species were listed on both unfertilised and fertilised swards. At this low to moderate level of exploitation intensity, the increased number of cuts had no significant negative effect on plant diversity either (19 species at 2 cuts vs. 20 species at 3 or 4 cuts). PK fertilisation increased the proportion of legumes in the herbage in the case of 2 or 3 cuts. The proportion of grasses in the herbage increased in all the fertilisation treatments with an increased numbers of cuts. Fertiliser treatment considerably reduced the proportion of marsh horsetail ( Equisetum palustre ) in the herbage of the meadows. This effect was even more pronounced at higher cut numbers. The proportion of Equisetum palustre in the herbage was the highest in the unfertilised sward with 2 cuts (26.4 %) and the lowest in the NPK-fertilised sward with 4 cuts (1.4%).

Vascular Plant Diversity of Forested Wetlands in Southern New England

Rhodora ◽  
10.3119/19-21 ◽  
2020 ◽  
Vol 122 (990) ◽  
Author(s):  
Laura Green ◽  
Marlyse Duguid
Keyword(s):  
New England ◽  
Plant Diversity ◽  
Vascular Plant ◽  
Forested Wetlands ◽  
Southern New England ◽  
Vascular Plant Diversity
Sign in / Sign up

Export Citation Format

Share Document