scholarly journals DNA metabarcoding reveals introduced species predominate in the diet of a threatened endemic omnivore, Telfair’s skink ( Leiolopisma telfairii )

2021 ◽  
Author(s):  
Maximillian P. T. G. Tercel ◽  
Rosemary J. Moorhouse‐Gann ◽  
Jordan P. Cuff ◽  
Lorna E. Drake ◽  
Nik C. Cole ◽  
...  
Keyword(s):  
Introduced Species ◽  
Dna Metabarcoding

A method for rearing Arhopalus ferus (Mulsant) (Coleoptera Cerambycidae) larvae on a modified artificial diet

2015 ◽  
Vol 68 ◽  
pp. 353-359 ◽  
Author(s):  
A.M. Barrington ◽  
D.P. Logan ◽  
P.G. Connolly
Keyword(s):  
Introduced Species ◽  
Artificial Diet ◽  
Larval Density ◽  
Pine Bark ◽  
Pine Wood ◽  
Rearing Method ◽  
Sawn Timber

Burnt pine longhorn (BPL) Arhopalus ferus (Mulsant) (Coleoptera Cerambycidae) is an introduced species sometimes found in association with export logs and sawn timber A rearing method was developed to produce larvae of a known age number and quality for control trials Growth of larvae from newly hatched to 5 weeks was measured on a standard cerambycid artificial diet and on modified diets Replacing pine wood with pine bark sawdust increased survival at 5 weeks from 23 to 76 and mean weight from 9 to 21 mg There were significant interactions between the influences of three factors (diet period of rearing initial larval density) on the weight of surviving larvae Individual rearing was preferred for convenience and a standardised method was used to rear 8740 larvae for disinfestation trials Establishment and survival to 6 weeks for these larvae was 97

COMPARATIVE STUDY BETWEEN FISH COLLECTION BY NATIONAL CENSUS ON RIVER ENVIRONMENT AND ENVIRONMENTAL DNA METABARCODING

2018 ◽  
Vol 74 (5) ◽  
pp. I_415-I_420 ◽  
Author(s):  
Yoshihisa AKAMATSU ◽  
Takayoshi TSUZUKI ◽  
Ryota YOKOYAMA ◽  
Yayoi FUNAHASHI ◽  
Munehiro OHTA ◽  
...  
Keyword(s):  
Comparative Study ◽  
Environmental Dna ◽  
Dna Metabarcoding ◽  
Fish Collection

Environmental DNA for functional diversity

2018 ◽  
Author(s):  
Pierre Taberlet ◽  
Aurélie Bonin ◽  
Lucie Zinger ◽  
Eric Coissac
Keyword(s):  
Functional Groups ◽  
Functional Diversity ◽  
Environmental Dna ◽  
Soil Organisms ◽  
Meaningful Information ◽  
Dna Metabarcoding ◽  
Pros And Cons ◽  
Target Community

Chapter 10 “Environmental DNA for functional diversity” discusses the potential of environmental DNA to assess functional diversity. It first focuses on DNA metabarcoding and discusses the extent to which this approach can be used and/or optimized to retrieve meaningful information on the functions of the target community. This knowledge usually involves coarsely defined functional groups (e.g., woody, leguminous, graminoid plants; shredders or decomposer soil organisms; pathogenicity or decomposition role of certain microorganisms). Chapter 10 then introduces metagenomics and metatranscriptomics approaches, their advantages, but also the challenges and solutions to appropriately sampling, sequencing these complex DNA/RNA populations. Chapter 10 finally presents several strategies and software to analyze metagenomes/metatranscriptomes, and discusses their pros and cons.

Environmental DNA

2018 ◽  
Author(s):  
Pierre Taberlet ◽  
Aurélie Bonin ◽  
Lucie Zinger ◽  
Eric Coissac
Keyword(s):  
Graduate Students ◽  
Feeding Habits ◽  
Environmental Dna ◽  
Research Field ◽  
Background Information ◽  
Ecological Processes ◽  
Biodiversity Research ◽  
Dna Metabarcoding ◽  
Standard Information

Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a particular focus on “eDNA metabarcoding”. Intended for scientists and managers, it provides the background information to allow the design of sound experiments. It revisits all steps necessary to produce high-quality metabarcoding data such as sampling, metabarcode design, optimization of PCR and sequencing protocols, as well as analysis of large sequencing datasets. All these different steps are presented by discussing the potential and current challenges of eDNA-based approaches to infer parameters on biodiversity or ecological processes. The last chapters of this book review how DNA metabarcoding has been used so far to unravel novel patterns of diversity in space and time, to detect particular species, and to answer new ecological questions in various ecosystems and for various organisms. Environmental DNA for biodiversity research and monitoring constitutes an essential reading for all graduate students, researchers and practitioners who do not have a strong background in molecular genetics and who are willing to use eDNA approaches in ecology and biomonitoring.

scholarly journals Pollen DNA metabarcoding identifies regional provenance and high plant diversity in Australian honey

2021 ◽  
Author(s):  
Liz Milla ◽  
Kale Sniderman ◽  
Rose Lines ◽  
Mahsa Mousavi‐Derazmahalleh ◽  
Francisco Encinas‐Viso
Keyword(s):  
Plant Diversity ◽  
Dna Metabarcoding

Estimating aquatic plant diversity and distribution in rivers from Jingjinji Region, China, using environmental DNA metabarcoding and a traditional survey method

2021 ◽  
pp. 111348
Author(s):  
Fenfen Ji ◽  
Liang Yan ◽  
Saihong Yan ◽  
Tianlong Qin ◽  
Jianzhong Shen ◽  
...  
Keyword(s):  
Plant Diversity ◽  
Aquatic Plant ◽  
Environmental Dna ◽  
Survey Method ◽  
Dna Metabarcoding

Environmental DNA metabarcoding as a useful tool for evaluating terrestrial mammal diversity in tropical forests

2021 ◽  
Author(s):  
José Luis Mena ◽  
Hiromi Yagui ◽  
Vania Tejeda ◽  
Emilio Bonifaz ◽  
Eva Bellemain ◽  
...  
Keyword(s):  
Tropical Forests ◽  
Environmental Dna ◽  
Terrestrial Mammal ◽  
Dna Metabarcoding ◽  
Mammal Diversity
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