Thermistor has a large temperature range, good stability and resistance to oxidation, which occupies an important position in the low-temperature measurement. This article describes new two-wire thermistor temperature measurement device using the Freescale MC9S12XS128 MCU with sampling capacitance. The device is composed of the MCU with its own A/D, MC9S12XS128 MCU, sampling capacitance, amplifier, boosted circuit, data acquisition and processing system, constant current source, etc. Using constant current source and 16-bit A/D converter designs the temperature measurement circuit, it can eliminate the effect from the conductor resistances in the traditional two-wire resistance temperature measurement system and reduce the measurement error which conductor resistances bring. The method is simple, practical, with high accuracy, strong anti-interference ability and other characteristics.