Formation and function of a complement-activating enzyme generated from factors of guinea pig serum and cobra venom

1971 ◽  
Vol 1 (4) ◽  
pp. 309-311 ◽  
Author(s):  
M. P. Dierich ◽  
D. Bitter-Suermann ◽  
W. König ◽  
U. Hadding
Keyword(s):  
Guinea Pig ◽  
Cobra Venom ◽  
And Function ◽  
Pig Serum

scholarly journals THE MECHANISM OF ANAPHYLATOXIN FORMATION

1914 ◽  
Vol 20 (1) ◽  
pp. 37-51 ◽  
Author(s):  
James W. Jobling ◽  
William Petersen
Keyword(s):  
Guinea Pig ◽  
Diphtheria Toxin ◽  
Serum Proteins ◽  
Horse Serum ◽  
Cobra Venom ◽  
Rabbit Serum ◽  
Partial Removal ◽  
The Matrix ◽  
Previously Treated ◽  
Pig Serum

1. The unsaturated lipoids (serum antitrypsin) can be adsorbed from guinea pig serum, rabbit serum, and horse serum by kaolin, starch, agar, and bacteria. 2. Diphtheria toxin and cobra venom also reduce the serum antitrypsin, possibly because of their affinity for lipoids. 3. Anaphylatoxins represent sera rendered toxic by partial removal of serum antitrypsin. 4. The matrix of the protein split products lies in the serum proteins so exposed. 5. The amount of removal of serum antitrypsin depends on definite quantitative relations; very large amounts and very small amounts of adsorbing substances are least effective (kaolin, starch, and bacteria). 6. Bacteria previously treated with serum or with oils do not adsorb serum antitrypsin. 7. Bacteria treated with serum become more resistant to the action of trypsin.

scholarly journals Phospholipid synthesis in HeLa cells exposed to immunoglobulin G and complement

1972 ◽  
Vol 128 (4) ◽  
pp. 953-960 ◽  
Author(s):  
Flemming Güttler
Keyword(s):  
Guinea Pig ◽  
Hela Cells ◽  
Immunoglobulin G ◽  
Phospholipid Content ◽  
Phospholipid Synthesis ◽  
Twofold Increase ◽  
And Function ◽  
Phosphate Incorporation ◽  
Pig Serum ◽  
Stimulation Of

1. HeLa cells were cultured in the presence of heterologous immunoglobulin G and guinea-pig serum together with [32P]phosphate. 2. Incorporation of [32P]phosphate was significantly stimulated by anti-HeLa immunoglobulin G and complement-sufficient serum compared with immunoglobulin G from unimmunized rabbits and complement. Within 2.5h heat-inactivated guinea-pig serum and anti-HeLa immunoglobulin G stimulated [32P]phosphate incorporation to the same extent as heat-inactivated complement and immunoglobulin G from unimmunized rabbits. 3. Compared with cells exposed to immunoglobulin G from unimmunized rabbits together with complement, anti-HeLa immunoglobulin G with complement increased the phospholipid content of HeLa cells twofold within 5h of incubation. 4. Exposure of HeLa cells to anti-HeLa immunoglobulin G and complement for 5–22h resulted in a twofold increase in the net accumulation of [32P]phosphate in sphingomyelin and phosphatidylcholine and a 50% increase in the net accumulation of [32P]phosphate in phosphatidylethanolamine, compared with cultures exposed to immunoglobulin G from unimmunized rabbits and complement. 5. A transient accumulation of 32P-labelled lysophosphoglycerides in HeLa cells exposed to antibody and complement was detected, confirming previous findings (Güttler & Clausen, 1969b). 6. The stimulation of [32P]phosphate turnover occurred in cells filling up their cytoplasma with vacuoles. This supports the suggestion that the accumulation of phospholipid in these cells may be concerned with the synthesis and function of cytomembranes.

scholarly journals HEAT LABILE OPSONINS TO PNEUMOCOCCUS

1969 ◽  
Vol 130 (6) ◽  
pp. 1229-1241 ◽  
Author(s):  
Hyun S. Shin ◽  
Mary Ruth Smith ◽  
W. Barry Wood
Keyword(s):  
Guinea Pig ◽  
The Other ◽  
Cobra Venom ◽  
Opsonic Activity ◽  
Cobra Venom Factor ◽  
Heat Labile ◽  
Deficient Mice ◽  
Pig Serum ◽  
Radio Labeling

When encapsulated type 25 pneumococci (Pn25) were opsonized with normal guinea pig serum, they consumed much more C3 than other complement (C) components. Fixation of C3 to the organisms was demonstrated by radio-labeling techniques, and its capsular localization was established by the use of monospecific anti-C3 antibody. Treatment of the serum with an appropriate dose of a purified cobra venom factor (VF) destroyed C3 and all of the opsonic activity, without appreciably affecting the other C components. Addition of purified C3 completely restored the opsonic activity of the VF-treated serum, indicating a requirement for C3. Since purified C3 alone had no opsonic activity, it was concluded that the C3 molecules had to be cleaved (to C3b) to function as opsonins. Experiments with C5-deficient mice revealed that C5 also plays a definite, but quantitatively less impressive, role in antipneumococcal defense.

Effect of Bentonite on Fibrinolytic System in Serum

1963 ◽  
Vol 10 (01) ◽  
pp. 120-132 ◽  
Author(s):  
E. S Olesen
Keyword(s):  
Guinea Pig ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
High Potential ◽  
Fibrinolytic System ◽  
Fibrinolytic Agents ◽  
Isoelectric Precipitation ◽  
Active Protease ◽  
Pig Serum

SummaryTreatment of serum with bentonite led to a reduced content of inhibitors of trypsin and urokinase in the isoelectrically precipitated euglobulin, and removed fibrinolytic agents and precursors from serum. Bentonite-treated serum added to untreated serum reduced precipitation of the above inhibitors, and presumably also precipitation of inhibitors against a plasminogen activator of serum.Bentonite-treated serum (whether from pig, ox, guinea-pig, or man), added to untreated guinea-pig serum, produced fibrinolytic activity on isoelectric precipitation of the mixture; the activity of the euglobulin was due to an activator of plasminogen as well as an active protease, probably plasmin. The described effects of bentonite-treated serum are similar to those previously reported for anionic polyelectrolytes. Possible mechanisms are discussed.The “non-specific” activation of fibrinolytic activity by means of bentonite emphasizes that guinea-pig serum [which is characterized by a high potential for “nonspecific” activation of its fibrinolytic system Olesen (1962)] contains all the elements required for the formation of an activator of plasminogen, and thus the activation of its plasminogen to plasmin.

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

Electrophoretic Studies of Normal Rovine and Guinea-Pig Serum

1963 ◽  
Vol 4 (1) ◽  
pp. 64-84
Author(s):  
I. Koefoed Jensen
Keyword(s):  
Guinea Pig ◽  
Pig Serum

scholarly journals Guinea Pig Serum and Liver l-Asparaginases

1970 ◽  
Vol 245 (11) ◽  
pp. 2797-2801 ◽  
Author(s):  
Helga M. Suld ◽  
Peter A. Herbut
Keyword(s):  
Guinea Pig ◽  
Pig Serum

W1363 Real Time Detection and Function of Melatonin Release from the Mucosa of Guinea Pig Ileum

2008 ◽  
Vol 134 (4) ◽  
pp. A-688 ◽  
Author(s):  
Bhavik A. Patel ◽  
Xiaochun Bian ◽  
James Galligan ◽  
Greg M. Swain
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Guinea Pig Ileum ◽  
Real Time Detection ◽  
And Function

scholarly journals EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

1963 ◽  
Vol 118 (1) ◽  
pp. 99-120 ◽  
Author(s):  
J. D. Broome
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Serum Proteins ◽  
Deae Cellulose ◽  
Salt Precipitation ◽  
Asparaginase Activity ◽  
Pig Serum

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.

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