Soluble immune complex triggering of a respiratory burst in macrophages: the role of complex aggregation at the phagocyte surface

Numbers of immunological approach have been made to the amyloidosis through the variety of predisposing human diseases and the experimentally induced animals by the greater number of agents. The results suggest an important role of impaired immunity involving both humoral and cell-mediated aspects.Recently the author has succeeded in producing amyloidosis in the rabbits and mice by the injections of immune complex of heat denatured DNA.The aim of this report is to demonstrate the details of the ultrastructure of the amyloidosis induced by heterologous insoluble immune complex. Eleven of twelve mice, dd strain, subcutaneously injected twice a week with Freund's complete adjuvant and four of seven animals intraperitonially injected developed systemic amyloidosis two months later from the initial injections. The spleens were electron microscopically observed.

Download Full-text

The role of adhesive interactions and extracellular matrix fibronectin from human polymorphonuclear leukocytes in the respiratory burst

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/0167-4889(91)90187-3 ◽

1991 ◽

Vol 1091 (3) ◽

pp. 257-260 ◽

Cited By ~ 10

Author(s):

G.F. Sud'ina ◽

A.V. Tatarintsev ◽

A.A. Koshkin ◽

S.V. Zaitsev ◽

N.A. Fedorov ◽

...

Keyword(s):

Extracellular Matrix ◽

Respiratory Burst ◽

Polymorphonuclear Leukocytes ◽

Adhesive Interactions ◽

Human Polymorphonuclear Leukocytes

Download Full-text

The Flavonols Quercetin, Myricetin, Kaempferol, and Galangin Inhibit the Net Oxygen Consumption by Immune Complex- Stimulated Human and Rabbit Neutrophils

Zeitschrift für Naturforschung C ◽

10.5560/znc.2012-0122 ◽

2014 ◽

Vol 69 (7-8) ◽

pp. 346-356 ◽

Cited By ~ 6

Author(s):

Andréa S. G. Figueiredo-Rinhel ◽

Everton O. L. Santos ◽

Luciana M. Kabeya ◽

Ana Elisa C. S. Azzolini ◽

Livia M. C. Simões-Ambrosio ◽

...

Keyword(s):

Oxygen Consumption ◽

Immune Complex ◽

Respiratory Burst ◽

Human Neutrophil ◽

Inflammatory Diseases ◽

Cell Types ◽

Oxygen Electrode ◽

Experimental Models ◽

Human Neutrophils ◽

Experimental Conditions

Stimulated human neutrophils exhibit increased net oxygen consumption (NOC) due to the conversion of O2 into the superoxide anion by the NADPH oxidase enzymatic complex during the respiratory burst. In several inflammatory diseases, overproduction of these oxidants causes tissue damage. The present study aims to: (a) optimize the experimental conditions used to measure the NOC in serum-opsonized zymosan (OZ)-and insoluble immune complex (i-IC)-stimulated human and rabbit neutrophils; and (b) compare the effect of four flavonols (quercetin, myricetin, kaempferol, and galangin) on this activity. We used a Clark-type oxygen electrode to measure the NOC of stimulated neutrophils. Eliciting the neutrophil respiratory burst with OZ and i-IC yielded similar maximum O2 uptake levels within the same species, but the human neutrophil NOC was almost four times higher than the rabbit neutrophil NOC. The optimal experimental conditions established for both cell types were 4·106 neutrophils mL-1, 2 mg mL-1 OZ, and 240 µg mL-1 i-IC. Upon stimulation with OZ or i-IC, the tested flavonols reduced the human and rabbit neutrophil NOC in the same order of potency - quercetin and galangin were the most and the least potent, respectively. These compounds were around four times more effective in inhibiting the rabbit as compared to the human neutrophil NOC, respectively. The four flavonols were not toxic to human or rabbit neutrophils. The experimental conditions used are suitable for both the determination of human and rabbit neutrophil NOC and for the assessment of the modulatory effects of natural compounds on these activities. The relationship between the level of NOC and the inhibitory potency of the flavonols suggests that rabbit neutrophils can be useful experimental models to predict the effect of drugs on immune complexstimulated human neutrophils.

Download Full-text