Cloning and sequencing of human λ immunoglobulin genes by the polymerase chain reaction

1990 ◽  
Vol 20 (12) ◽  
pp. 2661-2666 ◽  
Author(s):  
Sirirurg Songsivilai ◽  
Jacqueline M. Bye ◽  
James D. Marks ◽  
Nevin C. Hughes-Jones
Keyword(s):  
Polymerase Chain Reaction ◽  
Immunoglobulin Genes ◽  
Chain Reaction ◽  
Cloning And Sequencing ◽  
Polymerase Chain

Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction

1993 ◽  
Vol 23 (1) ◽  
pp. 206-211 ◽  
Author(s):  
Catherine A. Kettleborough ◽  
José Saldanha ◽  
Keith H. Ansell ◽  
Mary M. Bendig
Keyword(s):  
Polymerase Chain Reaction ◽  
Immunoglobulin Genes ◽  
Chain Reaction ◽  
Mouse Immunoglobulin ◽  
Polymerase Chain

Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells using mixed primers and the polymerase chain reaction

1989 ◽  
Vol 160 (3) ◽  
pp. 1250-1256 ◽  
Author(s):  
James W. Larrick ◽  
Lena Danielsson ◽  
Carol A. Brenner ◽  
Magnus Abrahamson ◽  
Kirk E. Fry ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hybridoma Cells ◽  
Immunoglobulin Genes ◽  
Chain Reaction ◽  
Polymerase Chain

Cloning and sequencing of a cDNA encoding an ovine oestrus-associated oviducal protein

1996 ◽  
Vol 8 (2) ◽  
pp. 305 ◽  
Author(s):  
JT Marshall ◽  
CD Nancarrow ◽  
AG Brownlee
Keyword(s):  
Amino Acids ◽  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Northern Blot ◽  
Mature Protein ◽  
Chain Reaction ◽  
Cloning And Sequencing ◽  
Glycosylation Sites ◽  
Polymerase Chain

Ovine oestrus-associated oviducal glycoprotein (oEGP) is synthesized and secreted specifically by the ampullary region of the ovine oviduct during the peri-ovulatory stages of the oestrous cycle. A cDNA that encodes oEGP was isolated and sequenced. Isolation of oEGP was achieved using the polymerase chain reaction (PCR) with primers based on a bovine oestrus-associated oviducal glycoprotein cDNA (bOGP) sequence. A 1599-bp cDNA encodes, in part, a deduced 519-amino acid sequence of mature protein which carries two potential N-linked glycosylation sites. The deduced amino acid sequence is more than 95% identical to that of bOGP and more than 74% identical to the first 491 amino acids of human oestrogen-dependent oviducal glycoprotein (hOGP). Northern blot hybridizations of RNA from several sheep tissues detected mRNA (2.4 kb) only in an ampulla oviduct sample.

Primers for polymerase chain reaction to detect genomic DNA of Toxocara canis and T. cati

1997 ◽  
Vol 71 (1) ◽  
pp. 77-78 ◽  
Author(s):  
Zhiliang Wu ◽  
Isao Nagano ◽  
Dong Xu ◽  
Yuzo Takahashi
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Random Amplified Polymorphic Dna ◽  
Toxocara Canis ◽  
Chain Reaction ◽  
Cloning And Sequencing ◽  
Polymerase Chain

AbstractPrimers for polymerase chain reaction to amplify genomic DNA of both Toxocara canis and T. cati were constructed by adapting cloning and sequencing random amplified polymorphic DNA. The primers are expected to detect eggs and/or larvae of T. canis and T. cati, both of which are known to cause toxocariasis in humans.

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