In vitro Degradation of 2,4,6-Trinitrotoluene Using Plant Tissue Cultures ofSolanum aviculare andRheum palmatum

The ability of plant cells cultivated in vitro to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism.

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Growth of Plant Tissue Cultures in Ultra-high Levels of Carbon Dioxide under Autotrophic and Heterotrophic Conditions

HortScience ◽

10.21273/hortsci.31.4.587e ◽

1996 ◽

Vol 31 (4) ◽

pp. 587e-587

Author(s):

Brent Tisserat ◽

Robert Silman

Keyword(s):

Plant Tissue ◽

Gas Permeability ◽

Tissue Cultures ◽

Natural Sunlight ◽

Sterile Culture ◽

Growth Modes ◽

Plant Tissue Cultures ◽

Culture Growth ◽

Almost All

A comparative study was undertaken to determine the influence of lighting, carbohydrate concentrations and ultra-high levels of CO2, i.e., >10,000 ppm, on sterile culture growth. Past CO2-sterile studies have confirmed that elevation of CO2 to as high as 1000 ppm resulted in beneficial growth. Within special constructed chambers, tissue cultures were given a variety of CO2 levels for 12–16 hours/day using artificial lighting and natural sunlight. Several different plants (lettuce, beans, pine) and plant culture types were grown in CO2-enriched environments, ranging from 350 to 50,000 ppm. In almost all cases, plant tissue cultures not only tolerated but exhibited enhanced growth using ultra-high levels of CO2. For example, lettuce cultures were found to grow 2 to 4 times faster under ultra-high CO2. levels than under normal atmospheric CO2 levels, i.e., 350 ppm. Natural sunlight was found to be suitable for sterile culture growth. Modes of administration of CO2 in vitro and gas permeability of various culture vessels are presented.

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In vitro plant tissue cultures accumulate polyisoprenoid alcohols.

Acta Biochimica Polonica ◽

10.18388/abp.2007_3184 ◽

2007 ◽

Vol 54 (4) ◽

pp. 847-852 ◽

Cited By ~ 3

Author(s):

Karolina Skorupinska-Tudek ◽

Anna Pytelewska ◽

Monika Zelman-Femiak ◽

Jakub Mikoszewski ◽

Olga Olszowska ◽

...

Keyword(s):

Cell Cultures ◽

Plant Tissue ◽

Plant Cells ◽

Ocimum Sanctum ◽

Taxus Baccata ◽

Root Cultures ◽

Tissue Cultures ◽

Cultivated Plant ◽

Plant Tissue Cultures

In vitro cultivated plant cells and tissues were found to synthesize polyisoprenoids. Taxus baccata suspension cell cultures accumulated polyisoprenoids of the same pattern as the parental tissue; methyl jasmonate or chitosan treatment almost doubled their content. All the root cultures studied accumulated dolichols as predominant polyisoprenoids. Roots of Ocimum sanctum grown in vitro accumulated approx. 2.5-fold higher amount of dolichols than the roots of soil-grown plants. Dolichols dominated over polyprenols in all Triticum sp. tissues studied.

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ACTION OF LIGHT AND TEMPERATURE ON GROWTH OF PLANT TISSUE CULTURES IN VITRO

American Journal of Botany ◽

10.1002/j.1537-2197.1955.tb10436.x ◽

1955 ◽

Vol 42 (10) ◽

pp. 869-873 ◽

Cited By ~ 10

Author(s):

Luigi de Capite

Keyword(s):

Plant Tissue ◽

Tissue Cultures ◽

Plant Tissue Cultures

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Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002798 ◽

1980 ◽

Vol 38 ◽

pp. 636-637

Author(s):

R. D. Sjolund ◽

C. Y. Shih

Keyword(s):

Plant Tissue ◽

Cultured Cells ◽

Freeze Fracture ◽

Tissue Cultures ◽

Sieve Elements ◽

Intact Plants ◽

Plant Tissue Cultures ◽

Whole Plants ◽

Energy Dependent ◽

Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

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