Abstract. Conventional techniques to measure boundary layer clouds such as research aircrafts are unable to sample in orographic or densely-populated areas. In this paper, we present a newly developed measurement platform on a tethered balloon system (HoloBalloon) to measure in situ vertical profiles of microphysical and meteorological cloud properties up to 1 kilometer above ground. The main component of the HoloBalloon platform is a holographic imager, which uses digital in-line holography to image cloud particles in a velocity independent sample volume, making it particularly well suited for measurements on a balloon. The unique combination of holography and balloon-borne measurements allows observations with high spatial resolution, covering cloud structures from the kilometer down to the millimeter scale. We present observations of a supercooled low stratus cloud (high fog event) during a Bise situation over the Swiss Plateau in February 2018. In situ microphysical profiles up to 700 m altitude above the ground and at temperatures down to −8 °C and wind speeds up to 15 m s−1 were performed. We were able to capture unique microphysical features from the kilometer down to the meter scale. For example, we observed cloud regions with decreased cloud droplet number concentration (CDNC) and cloud droplet size at scales of 30–50 meters. These cloud inhomogeneities could arise from adiabatic compression and heating and subsequent droplet evaporation in descending air parcels. Moreover, we observed conditions favorable for the formation of boundary layer waves and Kelvin-Helmholtz instability at the cloud top. This potentially influenced the cloud structure on a scale of 10–30 kilometers, which is reflected in the variability of the CDNC.