Biotransformation of the 8:2 fluorotelomer acrylate in rainbow trout. 1. In vivo dietary exposure

2010 ◽  
Vol 29 (12) ◽  
pp. 2726-2735 ◽  
Author(s):  
Craig M. Butt ◽  
Derek C.G. Muir ◽  
Scott A. Mabury
Keyword(s):  
Rainbow Trout ◽  
Dietary Exposure

scholarly journals Influence of citrate and PEG coatings on the bioaccumulation of TiO2 and CeO2 nanoparticles following dietary exposure in rainbow trout

2022 ◽  
Vol 34 (1) ◽  
Author(s):  
Mona Connolly ◽  
David Hernández-Moreno ◽  
Estefanía Conde ◽  
Alicia Garnica ◽  
José M. Navas ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Metal Oxide ◽  
Dietary Exposure ◽  
Fish Tissues ◽  
Aggregation State ◽  
Needed Information ◽  
The Relationship ◽  
Low Solubility ◽  
State Charge

Abstract Background This study was conducted to provide much needed information on the potential of low solubility, highly biodurable nanoparticles (NPs) (TiO2 and CeO2 NPs), to bioaccumulate in fish and to investigate the relationship between an engineered nanomaterials surface coating, uptake and biokinetics in vivo. Rainbow trout (Oncorhynchus mykiss) were fed diets spiked with uncoated or surface coated (polyethylene glycol (PEG) or citrate (CIT)) TiO2 and CeO2 NPs (100 mg NPs/kg feed) for 10 days and thereafter fish were allowed to depurate for 42 days. Special care was taken to measure the real dispersed and actual administered concentrations, taking into consideration any potential losses from leaching, and to characterise the form (size, aggregation state, charge) of the NPs to which the fish were exposed. Results The coatings had an influence on levels of uptake and distributions. Most notably a higher uptake of PEG and CIT coated TiO2 and CeO2 NPs compared to fish exposed to uncoated materials was observed. The elimination of any Ti from tissues was rapid during the first day of depuration, whereas Ce levels remained in fish tissues (stomach, intestine and liver) with differences in depuration and redistribution of the three types of CeO2 NPs. However, no bioaccumulation potential for both tested metal oxide NPs in fish irrespective of coatings is expected according to BMF values < 1. Conclusions Distinct uptake, distribution and depuration kinetics in rainbow trout have been evidenced for different metal oxide NPs (TiO2 and CeO2 NPs). Coatings influenced uptake and in some cases led to slower depuration and distinct distributions, but do not make the uncoated NPs studied bioaccumulative (BMF > 1). Graphical Abstract

scholarly journals Hypocalcin from Stannius corpuscles inhibits gill calcium uptake in trout

1988 ◽  
Vol 254 (6) ◽  
pp. R891-R896 ◽  
Author(s):  
F. P. Lafeber ◽  
G. Flik ◽  
S. E. Wendelaar Bonga ◽  
S. F. Perry
Keyword(s):  
Rainbow Trout ◽  
Calcium Uptake ◽  
Inhibitory Effect ◽  
Whole Body ◽  
Transepithelial Potential ◽  
Low Calcium ◽  
Stannius Corpuscles ◽  
Normal Calcium

Bidirectional whole body flux and branchial Ca2+ influx were measured in freshwater rainbow trout. Intra-arterial injections of homogenates of Stannius corpuscles (CS) as well as of a 54-kDa isolated product (hypocalcin) exerted an inhibitory effect on whole body Ca2+ influx, but did not effect Ca2+ efflux. Hypocalcin was more effective in reducing Ca2+ influx in trout acclimated to low-calcium freshwater than in fish from normal-calcium water. We conclude that the isolated product (hypocalcin) represents the hypocalcemic principle of the CS. Similar doses of hypocalcin caused quantitatively similar decreases in Ca2+ influx in vivo and in the isolated perfused head preparation. This indicates that the gills form the principle target for hypocalcin in trout. The branchial transepithelial potential did not change during hormone treatments. Possible mechanisms of hypocalcin action are suggested.

In Vivo and In Vitro Debromination of Decabromodiphenyl Ether (BDE 209) by Juvenile Rainbow Trout and Common Carp

2006 ◽  
Vol 40 (15) ◽  
pp. 4653-4658 ◽  
Author(s):  
Heather M. Stapleton ◽  
Brian Brazil ◽  
R. David Holbrook ◽  
Carys L. Mitchelmore ◽  
Rae Benedict ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Common Carp ◽  
Decabromodiphenyl Ether ◽  
Juvenile Rainbow Trout ◽  
Bde 209

Correlations between the RTG-2 cytotoxicity test EC50 and in vivo LC50 rainbow trout bioassay

Chemosphere ◽  
1996 ◽  
Vol 32 (11) ◽  
pp. 2141-2157 ◽  
Author(s):  
A. Castaño ◽  
M.J. Cantarino ◽  
P. Castillo ◽  
J.V. Tarazona
Keyword(s):  
Rainbow Trout ◽  
Cytotoxicity Test

Transport properties of cultured branchial epithelia from freshwater rainbow trout: a novel preparation with mitochondria-rich cells

2000 ◽  
Vol 203 (10) ◽  
pp. 1523-1537 ◽  
Author(s):  
M. Fletcher ◽  
S.P. Kelly ◽  
P. Part ◽  
M.J. O'Donnell ◽  
C.M. Wood
Keyword(s):  
Rainbow Trout ◽  
Flux Ratio ◽  
Culture Conditions ◽  
Chloride Cells ◽  
Pavement Cells ◽  
Ratio Criterion ◽  
Peg 4000 ◽  
Tight Epithelia ◽  
Gill Filaments

A new double-seeded insert (DSI) technique is described for culture of branchial epithelial preparations from freshwater rainbow trout on filter supports. DSI epithelia contain both pavement cells and mitochondria-rich (MR) cells (15.7+/−2.5 % of total cell numbers). MR cells occur singly or in clusters, are voluminous, open apically to the ‘external environment’ and exhibit ultrastructural characteristics similar to those found in the ‘chloride cells’ of freshwater fish gills. After 6–9 days in culture with Leibovitz's L-15 medium on both surfaces (symmetrical conditions), transepithelial resistance (TER) stabilized at values as high as 34 k capomega cm(2), indicative of electrically ‘tight’ epithelia. The density of MR cells, the surface area of their clusters and transepithelial potential (TEP; up to +8 mV basolateral positive, mean +1.9+/−0.2 mV) were all positively correlated with TER. In contrast, preparations cultured using an earlier single-seeded insert (SSI) technique contained only pavement cells and exhibited a negligible TEP under symmetrical conditions. Na(+)/K(+)-ATPase activities of DSI preparations were comparable with those in gill filaments, but did not differ from those of SSI epithelia. Replacement of the apical medium with fresh water to mimic the in vivo situation (asymmetrical conditions) induced a negative TEP (−6 to −15 mV) and increased permeability to the paracellular marker PEG-4000. Under symmetrical conditions, unidirectional Na(+) and Cl(−) fluxes were in balance, and there was no active transport by the Ussing flux ratio criterion. Under asymmetrical conditions, there were large effluxes, small influxes and evidence for active Cl(−) uptake and Na(+) extrusion. Unidirectional Ca(2+) fluxes were only 0.5-1.0 % of Na(+) and Cl(−) fluxes; active net Ca(2+) uptake occurred under symmetrical conditions and active net extrusion under asymmetrical conditions. Thus, DSI epithelia exhibit some of the features of the intact gill, but improvements in culture conditions are needed before the MR cells will function as true freshwater ‘chloride cells’.

Effects of epinephrine on branchial non-electrolyte permeability in rainbow trout

1978 ◽  
Vol 74 (1) ◽  
pp. 227-237 ◽  
Author(s):  
J. Isaia ◽  
J. Maetz ◽  
G. P. Haywood
Keyword(s):  
Rainbow Trout ◽  
Beta Blocker ◽  
Transport Mechanism ◽  
Constant Pressure ◽  
Salmo Gairdneri ◽  
Permeability Coefficients ◽  
Lipophilic Substance ◽  
Hydrophilic Solutes ◽  
Bulk Transport

Using isolated heads perfused at constant pressure, at rates close to those occurring in vivo, the permeability of the gills of the trout Salmo gairdneri to a range of solutes was measured. Under epinephrine-free conditions, butanol and water showed similar high branchial permeability coefficients. Urea, inulin and dextrans (mol. wt 3000 and 20 000) were 7–12 times less permeant, and mannitol 60-70 times less permeant than water or butanol. Epinephrine, at 10(−6) M, greatly increased the permeability of the gills to the small hydrophilic molecules, water and urea, and to the lipophilic substance, butanol, but did not affect the penetration of the large hydrophilic solutes, mannitol, inulin and dextrans. In the presence of 10(−6) M propanolol, a beta-blocker, epinephrine had no effect on the permeation of any of the test substances except that the permeability to urea decreased somewhat. The results suggest that epinephrine increases the permeability of the membranes of the branchial cells but does not affect the permeation of substances that cross the gill walls by paracellular routes or via an intracellular ‘bulk-transport’ mechanism. Such an action would be expected to increase the branchial transfer of oxygen.

scholarly journals Bioaccumulation of metals in juvenile rainbow trout (oncorhynchus mykiss) via dietary exposure to blue mussels

Chemosphere ◽  
2017 ◽  
Vol 188 ◽  
pp. 548-556 ◽  
Author(s):  
Gillian McEneff ◽  
Brian Quinn ◽  
Matthew Bennion ◽  
Sorcha Dolan ◽  
Kathleen O'Rourke ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Dietary Exposure ◽  
Blue Mussels ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Estrogen-Like Activity of Perfluoroalkyl Acids In Vivo and Interaction with Human and Rainbow Trout Estrogen Receptors In Vitro

2010 ◽  
Vol 120 (1) ◽  
pp. 42-58 ◽  
Author(s):  
Abby D. Benninghoff ◽  
William H. Bisson ◽  
Daniel C. Koch ◽  
David J. Ehresman ◽  
Siva K. Kolluri ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Estrogen Receptors ◽  
Perfluoroalkyl Acids

Palmitate movement across red and white muscle membranes of rainbow trout

2004 ◽  
Vol 286 (1) ◽  
pp. R46-R53 ◽  
Author(s):  
Jeff G. Richards ◽  
Arend Bonen ◽  
George J. F. Heigenhauser ◽  
Chris M. Wood
Keyword(s):  
Fatty Acid ◽  
Rainbow Trout ◽  
White Muscle ◽  
Specific Inhibitor ◽  
Fatty Acid Binding ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Fatty Acid Transporter ◽  
Palmitate Uptake ◽  
High Concentrations

We examined the movement of [3H]palmitate across giant sarcolemmal vesicles prepared from red and white muscle of rainbow trout ( Oncorhynchus mykiss). Red and white muscle fatty acid carriers have similar affinities for palmitate (apparent Km = 26 ± 6 and 33 ± 8 nM, respectively); however, red muscle has a higher maximal uptake compared with white muscle ( Vmax = 476 ± 41 vs. 229 ± 23 pmol·mg protein-1·s-1, respectively). Phloretin (250 μM) inhibited palmitate influx in red and white muscle vesicles by ∼40%, HgCl2 (2.5 mM) inhibited palmitate uptake by 20-30%, and the anion-exchange inhibitor DIDS (250 μM) inhibited palmitate influx in red and white muscle vesicles by ∼15 and 30%, respectively. Western blot analysis of red and white muscle vesicles did not detect a mammalian-type fatty acid transporter (FAT); however, preincubation of vesicles with sulfo- N-succinimidyloleate, a specific inhibitor of FAT in rats, reduced palmitate uptake in red and white muscle vesicles by ∼15 and 25%, respectively. A mammalian-type plasma membrane fatty acid-binding protein was identified in trout muscle using Western blotting, but the protein differed in size between red and white muscle. At low concentrations of free palmitate (2.5 nM), addition of high concentrations (111 μM total) of oleate (18:0) caused ∼50% reduction in palmitate uptake by red and white muscle vesicles, but high concentrations (100 μM) of octanoate (8:0) caused no inhibition of uptake. Five days of aerobic swimming at ∼2 body lengths/s and 9 days of chronic cortisol elevation in vivo, both of which stimulate lipid metabolism, had no effect on the rate of palmitate movement in red or white muscle vesicles.

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