scholarly journals A novel approach for modifying tumor cell-derived plasma membrane vesicles to contain encapsulated IL-2 and engrafted costimulatory molecules for use in tumor immunotherapy

2002 ◽  
Vol 98 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Christina L. van Broekhoven ◽  
Joseph G. Altin
2020 ◽  
Author(s):  
Erdinc Sezgin ◽  
Dario Carugo ◽  
Ilya Levental ◽  
Eleanor Stride ◽  
Christian Eggeling

AbstractModel membrane systems are essential tools for biology, enabling study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between native cellular plasma and artificial membranes. Certain applications, however, require planar membrane surfaces. Here, we report a novel approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using an ultrasonic pressure field generated within an acoustofluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems will be useful for many applications requiring detailed characterization of plasma membrane dynamics.


2012 ◽  
Vol 33 (7) ◽  
pp. 535-542 ◽  
Author(s):  
Wenjun J. Sun ◽  
Mehri Kaviani Mogadam ◽  
Marianne Sommarin ◽  
Henrietta Nittby ◽  
Leif G. Salford ◽  
...  

Author(s):  
Nikolas K. Teiwes ◽  
Ingo Mey ◽  
Phila C. Baumann ◽  
Lena Strieker ◽  
Ulla Unkelbach ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document