Disappearance of a major cell-type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by rous sarcoma virus

1974 ◽  
Vol 13 (5) ◽  
pp. 579-586 ◽  
Author(s):  
Antti Vaheri ◽  
Erkki Ruoslahti
Keyword(s):  
Specific Surface ◽  
Rous Sarcoma Virus ◽  
Surface Glycoprotein ◽  
Cell Type ◽  
Rous Sarcoma ◽  
Major Cell Type ◽  
Glycoprotein Antigen ◽  
Sarcoma Virus ◽  
Cell Type Specific

scholarly journals DISTRIBUTION OF FIBROBLAST SURFACE ANTIGEN

1974 ◽  
Vol 140 (6) ◽  
pp. 1522-1533 ◽  
Author(s):  
Jorma Wartiovaara ◽  
Ewert Linder ◽  
Erkki Ruoslahti ◽  
Antti Vaheri
Keyword(s):  
Surface Antigen ◽  
Rous Sarcoma Virus ◽  
Growth Control ◽  
Trypsin Treatment ◽  
Membrane Processes ◽  
Rous Sarcoma ◽  
Cell Surface Structures ◽  
Sarcoma Virus ◽  
A Cell ◽  
Cell Type Specific

The localization of a cell type-specific, soluble fibroblast surface antigen (SFA) was studied by immunofluorescence and by scanning electron microscopy of the same cells. The antigen had an uneven distribution forming streaks on chick embryo fibroblasts. It was localized to membrane processes and ridges, with a diameter of 50–200 nm. The processes extended from the periphery of the cells to the substratum or to other cells. Trypsin treatment completely removed detectable amounts of SFA. The antigen was detectable within 1 h after trypsin-treated cells were reseeded. The reappearance of SFA correlated with the restoration of membrane processes. Fibroblasts transformed by Rous sarcoma virus (RSV) showed loss of all or most SFA. When normal cells were transformed without subcultivation and trypsinization a fibrillar extracellular network of SFA remained under the transformed fibroblasts while the cells themselves were negative in immunofluorescence. When fibroblasts infected by RSV mutants were transferred to nonpermissive temperature for transformation new SFA was detected within 2 h. These data lead us to propose that loss of stabilizing and anchoring effect of SFA molecules in fibrillar cell surface structures may be critical in altered growth control and malignant transformation.

Factors Influencing the Cell Type of Brain Tumors Induced in Dogs by Schmidt-Ruppin Rous Sarcoma Virus

1972 ◽  
Vol 31 (4) ◽  
pp. 583-595 ◽  
Author(s):  
Darell D. Bigner ◽  
John P. Kvedar ◽  
Thomas C. Shaffer ◽  
Nicholas A. Vick ◽  
W. King Engel ◽  
...  
Keyword(s):  
Brain Tumors ◽  
Rous Sarcoma Virus ◽  
Cell Type ◽  
Rous Sarcoma ◽  
Factors Influencing ◽  
Sarcoma Virus

Correlation between tumor-specific surface antigens and src gene expression in Rous sarcoma virus-induced rat tumors

1983 ◽  
Vol 19 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Noboru Kuzumaki ◽  
Hidetaka Minakawa ◽  
Tetsumichi Matsuo ◽  
Soichi Haraguchi ◽  
Takato O. Yoshida
Keyword(s):  
Gene Expression ◽  
Specific Surface ◽  
Rous Sarcoma Virus ◽  
Surface Antigens ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus ◽  
Rat Tumors

Role of the mitogenic property and kinase activity of p60src in tumor formation by Rous sarcoma virus.

1984 ◽  
Vol 49 (2) ◽  
pp. 325-332 ◽  
Author(s):  
F Poirier ◽  
P Jullien ◽  
P Dezelee ◽  
G Dambrine ◽  
E Esnault ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Kinase Activity ◽  
Tumor Formation ◽  
Rous Sarcoma ◽  
Sarcoma Virus
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