Nuclear DNA content, proliferating-cell nuclear antigen (PCNA) and p53 immunostaining in predicting progression of laryngeal cancer in situ lesions

2007 ◽  
Vol 56 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Eva Munck-Wikland ◽  
Staffan Edström ◽  
Eva Jungmark ◽  
Richard Kuylenstierna ◽  
Johan Lindholm ◽  
...  
Keyword(s):  
Laryngeal Cancer ◽  
Dna Content ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Nuclear Antigen ◽  
Proliferating Cell

Determination of nuclear DNA content in fungi using mithramycin: vegetative diploidy in Armillaria mellea confirmed

1983 ◽  
Vol 29 (9) ◽  
pp. 1179-1183 ◽  
Author(s):  
A. L. Franklin ◽  
W. G. Filion ◽  
J. B. Anderson
Keyword(s):  
Dna Binding ◽  
Aspergillus Nidulans ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Phytopathogenic Fungus ◽  
Armillaria Mellea ◽  
Dna Measurements

Armillaria mellea, a phytopathogenic fungus, is the only member of the Agaricales (Basidiomycetes) whose fertile vegetative phase in nature is thought to be diploid, rather than dikaryotic. To examine the vegetative ploidy of A. mellea, we used the DNA-binding antibiotic, mithramycin, for fluorometry of in situ nuclear DNA. The measurements of nuclear DNA content indicated that strains derived from single basidiospores of A. mellea were haploid and that strains derived from matings of isolates of single spores were diploid. These data confirm the results of earlier genetic experiments, which show haploidy and diploidy in unmated and mated strains, respectively. Nuclear DNA measurements in known haploid and diploid strains of Aspergillus nidulans confirmed the validity of our protocol.

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