scholarly journals Site-specific recombination in bacteriophage Mu: characterization of binding sites for the DNA invertase Gin.

1988 ◽  
Vol 7 (4) ◽  
pp. 1219-1227 ◽  
Author(s):  
G. Mertens ◽  
A. Klippel ◽  
H. Fuss ◽  
H. Blöcker ◽  
R. Frank ◽  
...  
Keyword(s):  
Binding Sites ◽  
Bacteriophage Mu ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Characterization of pABVA01, a Plasmid Encoding the OXA-24 Carbapenemase from Italian Isolates of Acinetobacter baumannii

2009 ◽  
Vol 53 (8) ◽  
pp. 3528-3533 ◽  
Author(s):  
Marco Maria D'Andrea ◽  
Tommaso Giani ◽  
Silvia D'Arezzo ◽  
Alessandro Capone ◽  
Nicola Petrosillo ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Acinetobacter Baumannii ◽  
Binding Sites ◽  
Inverted Repeats ◽  
Clonal Lineage ◽  
Recombination Mechanism ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Carbapenem Resistant

ABSTRACT Two epidemiologically unrelated carbapenem-resistant Acinetobacter baumannii isolates were investigated as representatives of the first Italian isolates producing the OXA-24 carbapenemase. Both isolates were of European clonal lineage II and carried an identical OXA-24-encoding plasmid, named pABVA01. Comparative analysis revealed that in pABVA01, bla OXA-24 was part of a DNA module flanked by conserved inverted repeats homologous to XerC/XerD binding sites, which in other Acinetobacter plasmids flank different DNA modules, suggesting mobilization by a novel site-specific recombination mechanism.

Characterization of Holliday structures in FLP protein-promoted site-specific recombination

1990 ◽  
Vol 10 (1) ◽  
pp. 235-242
Author(s):  
L Meyer-Leon ◽  
R B Inman ◽  
M M Cox
Keyword(s):  
Reaction Rate ◽  
Reaction Pathway ◽  
Reaction Intermediates ◽  
Wild Type ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Isomerization Process ◽  
Previous Demonstration ◽  
Rate Limiting

Holliday structures are formed in the course of FLP protein-promoted site-specific recombination. Here, we demonstrate that Holliday structures are formed in reactions involving wild-type substrates and that they are kinetically competent with respect to the overall reaction rate. Together with a previous demonstration of chemical competence (L. Meyer-Leon, L.-C. Huang, S. W. Umlauf, M. M. Cox, and R. B. Inman, Mol. Cell. Biol. 8:3784-3796, 1988), Holliday structures therefore meet all criteria necessary to establish that they are obligate reaction intermediates in FLP-mediated site-specific recombination. In addition, kinetic evidence suggests that two distinct forms of the Holliday intermediate are present in the reaction pathway, interconverted in an isomerization process that is rate limiting at 0 degree C.

scholarly journals Gin-mediated site-specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro

1984 ◽  
Vol 3 (10) ◽  
pp. 2415-2421 ◽  
Author(s):  
Gabriele Mertens ◽  
Andrea Hoffmann ◽  
Helmut Blöcker ◽  
Ronald Frank ◽  
Regine Kahmann
Keyword(s):  
Bacteriophage Mu ◽  
Site Specific ◽  
Site Specific Recombination ◽  
And Inversion

Comparison of the structure–activity relationships of the integron-associated recombination sites attI3 and attI1 reveals common features

Microbiology ◽  
2004 ◽  
Vol 150 (5) ◽  
pp. 1591-1601 ◽  
Author(s):  
Christina M. Collis ◽  
Ruth M. Hall
Keyword(s):  
Binding Sites ◽  
Crossover Point ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Gene Cassettes ◽  
Common Features ◽  
Base Element ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Interference Studies

Incorporation of gene cassettes into integrons occurs by IntI-mediated site-specific recombination between a 59-base element (59-be) site in the cassette and an attI site in the integron. While the 59-be sites share common features and are recognized by several different IntI recombinases, the sequences of attI sites are not obviously related and are preferentially recognized by the cognate IntI. To determine the features of attI sites that are required for recombination proficiency, the structure–activity relationships of a second attI site, the attI3 site from the class 3 integron, were examined. The attI3 site was confined to within a region consisting of 68 bp from the integron backbone and 15 bp from the adjacent cassette. This region includes four IntI3-binding sites, as assessed by gel shift and methylation interference studies. Two of the binding sites are inversely oriented and constitute a simple site that includes the recombination crossover point. The two additional binding sites appear to be directly oriented and one of them is essential for efficient recombination of the attI3 site with a 59-be, but not for recombination with a second full-length attI3 site, which occurs at 100-fold lower frequency. The fourth site enhances attI3 with 59-be recombination 10-fold. The finding that the organization and overall properties of attI3 are very similar to those of attI1 indicates that these features are likely to be common to all attI sites.

scholarly journals Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii

Parasitology ◽  
2014 ◽  
Vol 141 (11) ◽  
pp. 1390-1398 ◽  
Author(s):  
ELENA JIMÉNEZ-RUIZ ◽  
ELEANOR H. WONG ◽  
GURMAN S. PALL ◽  
MARKUS MEISSNER
Keyword(s):  
Toxoplasma Gondii ◽  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Advantages And Disadvantages ◽  
Gene Function Analysis ◽  
Tetracycline Inducible System

SUMMARYThe dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput.

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