scholarly journals Characterization of pABVA01, a Plasmid Encoding the OXA-24 Carbapenemase from Italian Isolates of Acinetobacter baumannii

2009 ◽  
Vol 53 (8) ◽  
pp. 3528-3533 ◽  
Author(s):  
Marco Maria D'Andrea ◽  
Tommaso Giani ◽  
Silvia D'Arezzo ◽  
Alessandro Capone ◽  
Nicola Petrosillo ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Acinetobacter Baumannii ◽  
Binding Sites ◽  
Inverted Repeats ◽  
Clonal Lineage ◽  
Recombination Mechanism ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Carbapenem Resistant

ABSTRACT Two epidemiologically unrelated carbapenem-resistant Acinetobacter baumannii isolates were investigated as representatives of the first Italian isolates producing the OXA-24 carbapenemase. Both isolates were of European clonal lineage II and carried an identical OXA-24-encoding plasmid, named pABVA01. Comparative analysis revealed that in pABVA01, bla OXA-24 was part of a DNA module flanked by conserved inverted repeats homologous to XerC/XerD binding sites, which in other Acinetobacter plasmids flank different DNA modules, suggesting mobilization by a novel site-specific recombination mechanism.

scholarly journals Site-specific recombination in bacteriophage Mu: characterization of binding sites for the DNA invertase Gin.

1988 ◽  
Vol 7 (4) ◽  
pp. 1219-1227 ◽  
Author(s):  
G. Mertens ◽  
A. Klippel ◽  
H. Fuss ◽  
H. Blöcker ◽  
R. Frank ◽  
...  
Keyword(s):  
Binding Sites ◽  
Bacteriophage Mu ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Characterization of carbapenem-resistant Acinetobacter baumannii isolates in a Chinese teaching hospital

2015 ◽  
Vol 6 ◽  
Author(s):  
Yaowen Chang ◽  
Guangxin Luan ◽  
Ying Xu ◽  
Yanhong Wang ◽  
Min Shen ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Teaching Hospital ◽  
Carbapenem Resistant ◽  
Chinese Teaching

Characterization of Holliday structures in FLP protein-promoted site-specific recombination

1990 ◽  
Vol 10 (1) ◽  
pp. 235-242
Author(s):  
L Meyer-Leon ◽  
R B Inman ◽  
M M Cox
Keyword(s):  
Reaction Rate ◽  
Reaction Pathway ◽  
Reaction Intermediates ◽  
Wild Type ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Isomerization Process ◽  
Previous Demonstration ◽  
Rate Limiting

Holliday structures are formed in the course of FLP protein-promoted site-specific recombination. Here, we demonstrate that Holliday structures are formed in reactions involving wild-type substrates and that they are kinetically competent with respect to the overall reaction rate. Together with a previous demonstration of chemical competence (L. Meyer-Leon, L.-C. Huang, S. W. Umlauf, M. M. Cox, and R. B. Inman, Mol. Cell. Biol. 8:3784-3796, 1988), Holliday structures therefore meet all criteria necessary to establish that they are obligate reaction intermediates in FLP-mediated site-specific recombination. In addition, kinetic evidence suggests that two distinct forms of the Holliday intermediate are present in the reaction pathway, interconverted in an isomerization process that is rate limiting at 0 degree C.

scholarly journals First Description of Colistin and Tigecycline-Resistant Acinetobacter baumannii Producing KPC-3 Carbapenemase in Portugal

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 96 ◽  
Author(s):  
Cátia Caneiras ◽  
Filipa Calisto ◽  
Gabriela Jorge da Silva ◽  
Luis Lito ◽  
José Melo-Cristino ◽  
...  
Keyword(s):  
Case Report ◽  
High Risk ◽  
Acinetobacter Baumannii ◽  
University Hospital ◽  
Virulence Determinants ◽  
Molecular Screening ◽  
Pcr Screening ◽  
Carbapenem Resistant ◽  
Antimicrobial Resistance Profile

Herein, we describe a case report of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae isolates that were identified from the same patient at a Tertiary University Hospital Centre in Portugal. Antimicrobial susceptibility and the molecular characterization of resistance and virulence determinants were performed. PCR screening identified the presence of the resistance genes blaKPC-3, blaTEM-1 and blaSHV-1 in both isolates. The KPC-3 K. pneumoniae isolate belonged to the ST-14 high risk clone and accumulated an uncommon resistance and virulence profile additional to a horizontal dissemination capacity. In conclusion, the molecular screening led to the first identification of the A. baumannii KPC-3 producer in Portugal with a full antimicrobial resistance profile including tigecycline and colistin.

Sign in / Sign up

Export Citation Format

Share Document