scholarly journals Isolation and characterization of intermediates in site-specific recombination.

1987 ◽  
Vol 84 (19) ◽  
pp. 6840-6844 ◽  
Author(s):  
R. Hoess ◽  
A. Wierzbicki ◽  
K. Abremski
Keyword(s):  
Site Specific ◽  
Site Specific Recombination ◽  
Isolation And Characterization

Characterization of Holliday structures in FLP protein-promoted site-specific recombination

1990 ◽  
Vol 10 (1) ◽  
pp. 235-242
Author(s):  
L Meyer-Leon ◽  
R B Inman ◽  
M M Cox
Keyword(s):  
Reaction Rate ◽  
Reaction Pathway ◽  
Reaction Intermediates ◽  
Wild Type ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Isomerization Process ◽  
Previous Demonstration ◽  
Rate Limiting

Holliday structures are formed in the course of FLP protein-promoted site-specific recombination. Here, we demonstrate that Holliday structures are formed in reactions involving wild-type substrates and that they are kinetically competent with respect to the overall reaction rate. Together with a previous demonstration of chemical competence (L. Meyer-Leon, L.-C. Huang, S. W. Umlauf, M. M. Cox, and R. B. Inman, Mol. Cell. Biol. 8:3784-3796, 1988), Holliday structures therefore meet all criteria necessary to establish that they are obligate reaction intermediates in FLP-mediated site-specific recombination. In addition, kinetic evidence suggests that two distinct forms of the Holliday intermediate are present in the reaction pathway, interconverted in an isomerization process that is rate limiting at 0 degree C.

scholarly journals Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii

Parasitology ◽  
2014 ◽  
Vol 141 (11) ◽  
pp. 1390-1398 ◽  
Author(s):  
ELENA JIMÉNEZ-RUIZ ◽  
ELEANOR H. WONG ◽  
GURMAN S. PALL ◽  
MARKUS MEISSNER
Keyword(s):  
Toxoplasma Gondii ◽  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Advantages And Disadvantages ◽  
Gene Function Analysis ◽  
Tetracycline Inducible System

SUMMARYThe dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput.

scholarly journals Characterization of pABVA01, a Plasmid Encoding the OXA-24 Carbapenemase from Italian Isolates of Acinetobacter baumannii

2009 ◽  
Vol 53 (8) ◽  
pp. 3528-3533 ◽  
Author(s):  
Marco Maria D'Andrea ◽  
Tommaso Giani ◽  
Silvia D'Arezzo ◽  
Alessandro Capone ◽  
Nicola Petrosillo ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Acinetobacter Baumannii ◽  
Binding Sites ◽  
Inverted Repeats ◽  
Clonal Lineage ◽  
Recombination Mechanism ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Carbapenem Resistant

ABSTRACT Two epidemiologically unrelated carbapenem-resistant Acinetobacter baumannii isolates were investigated as representatives of the first Italian isolates producing the OXA-24 carbapenemase. Both isolates were of European clonal lineage II and carried an identical OXA-24-encoding plasmid, named pABVA01. Comparative analysis revealed that in pABVA01, bla OXA-24 was part of a DNA module flanked by conserved inverted repeats homologous to XerC/XerD binding sites, which in other Acinetobacter plasmids flank different DNA modules, suggesting mobilization by a novel site-specific recombination mechanism.

Cellular factors couple recombination with growth phase: Characterization of a new component in the λ site-specific recombination pathway

Cell ◽  
1987 ◽  
Vol 50 (6) ◽  
pp. 901-908 ◽  
Author(s):  
John F. Thompson ◽  
Lina Moitoso de Vargas ◽  
Christian Koch ◽  
Regine Kahmann ◽  
Arthur Landy
Keyword(s):  
Growth Phase ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Cellular Factors ◽  
Recombination Pathway ◽  
Phase Characterization

scholarly journals In vivo and in vitro characterization of site-specific recombination of actinophage R4 integrase

2011 ◽  
Vol 57 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Takamasa Miura ◽  
Yayoi Hosaka ◽  
Yang Yan-Zhuo ◽  
Tomoyasu Nishizawa ◽  
Munehiko Asayama ◽  
...  
Keyword(s):  
Site Specific ◽  
Site Specific Recombination ◽  
In Vitro Characterization

scholarly journals Site-Specific Recombination with the Chromosomal tRNALeu Gene by the Large Conjugative Haemophilus Resistance Plasmid

2002 ◽  
Vol 46 (5) ◽  
pp. 1602-1603 ◽  
Author(s):  
Ioanna D. Dimopoulou ◽  
Joanne E. Russell ◽  
Zaini Mohd-Zain ◽  
Rebecca Herbert ◽  
Derrick W. Crook
Keyword(s):  
Haemophilus Influenzae ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Resistance Plasmid ◽  
Diverse Origin

ABSTRACT Characterization of the sequences involved in recombination of the Haemophilus plasmid p1056 with the Haemophilus influenzae chromosome produced evidence indicating site-specific recombination with chromosomal tRNALeu. attP sequences identical to those of p1056 were found in six plasmids of diverse origin, suggesting that a family of Haemophilus plasmids recombines with chromosomal tRNALeu.

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