Extracellular signal-regulated kinase activation in the chronic constriction injury model of neuropathic pain in anaesthetized rats

Neuropathic pain is common in clinical practice. Exploration of new drug therapeutics has always been carried out for more satisfactory effects and fewer side-effects. In the present study, we aimed to investigate effects of Tongluo Zhitong Prescription (TZP), a compounded Chinese medicine description, on neuropathic pain model of rats with chronic constriction injury (CCI). The CCI model was established by loosely ligating sciatic nerve with catgut suture, proximal to its trifurcation. The static and dynamic allodynia, heat hyperalgesia, mechanical allodynia, cold allodynia, and gait were assessed. Our results showed that TZP alleviated CCI-induced static and dynamic allodynia, suppressed heat hyperalgesia and cold and mechanical allodynia, and improved gait function. These results suggest that TZP could alleviate neuropathic pain. Further experiments are needed to explore its mechanisms.

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Senkyunolide I Relieves Neuropathic Pain Induced by Chronic Constriction Injury by Inhibiting Activation of Microglia

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.20:253-258 ◽

2021 ◽

Vol 20 (2) ◽

pp. 253-258

Author(s):

Xiaomin Huang ◽

Miao Huo

Keyword(s):

Neuropathic Pain ◽

Chronic Constriction Injury ◽

Biological Activities ◽

Bioactive Components ◽

Extracellular Signal Regulated Kinase ◽

Extracellular Signal ◽

Induced Apoptosis ◽

Kinase Pathway ◽

Senkyunolide I

As an alternative to the use of narcotics, generally refractory to long-term effectiveness, for the management of neuropathic pain, we have explored the utility of senkyunolide I. Senkyunolide I is one of the bioactive components isolated from Ligusticum chuanxiong Hort known to exhibit multiple biological activities. In this study, we report senkyunolide I inhibition of chronic constriction injury induced neuropathic pain. Mechanistically, senkyunolide I inhibited chronic constriction injury induced apoptosis and the activity of microglia via extracellular signal regulated kinase pathway. We therefore suggest that senkyunolide I could serve as a promising drug for the treatment of neuropathic pain.

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Selective loss of spinal GABAergic or glycinergic neurons is not necessary for development of thermal hyperalgesia in the chronic constriction injury model of neuropathic pain

Pain ◽

10.1016/s0304-3959(03)00011-3 ◽

2003 ◽

Vol 104 (1) ◽

pp. 229-239 ◽

Cited By ~ 162

Author(s):

E Polgár ◽

I D. Hughes ◽

S J. Riddell ◽

J D. Maxwell ◽

Z Puskár ◽

...

Keyword(s):

Neuropathic Pain ◽

Chronic Constriction Injury ◽

Thermal Hyperalgesia ◽

Selective Loss ◽

Injury Model ◽

Chronic Constriction Injury Model

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Comparative efficacy of pregabalin and baclofen in the rodent chronic constriction injury model of neuropathic pain

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20185171 ◽

2018 ◽

Vol 8 (1) ◽

pp. 133

Author(s):

Saurabh Kohli ◽

Taruna Sharma ◽

Juhi Kalra ◽

Dilip C. Dhasmana

Keyword(s):

Neuropathic Pain ◽

Sciatic Nerve ◽

Chronic Constriction Injury ◽

Thermal Hyperalgesia ◽

Mechanical Hyperalgesia ◽

Comparative Efficacy ◽

First Line ◽

Injury Model ◽

Different Types ◽

Chronic Constriction Injury Model

Background: Neuropathic pain is associated with prolonged disability and is usually not responsive to conventional analgesics like NSAIDs and opioids. Even the recommended first-line drugs are effective in less than 50% patients. Thus, drugs with different mechanisms of action are needed. Baclofen, a GABA-B agonist has shown benefit in different types of neuropathic pains and is compared against pregabalin.Methods: The sciatic nerve was ligated in 2 groups of 6 rats each as per the chronic constriction injury model of neuropathic pain on day 0. After 14 days the effect of single doses of pregabalin (30mg/kg) and baclofen (5mg/kg) intraperitoneally were assessed over a 2 hours period. Thermal and mechanical hyperalgesia were assessed as measures of neuropathic pain by the hotplate and pin-prick method respectively.Results: Significant thermal and mechanical hyperalgesia was produced 14 days after sciatic nerve ligation in both the groups (p <0.05). Both pregabalin (p <0.001) and baclofen (p <0.01) were effective in decreasing thermal hyperalgesia throughout the two hours study period, but pregabalin was more effective as compared to baclofen (p <0.05) at 30, 60 and 120minutes. Both the drugs produced a significant decrease in mechanical hyperalgesia (p <0.01) throughout the study period. Again, pregabalin was the more effective drug (p <0.05) at all time points.Conclusions: Significant thermal and mechanical hyperalgesia was seen 14 days after sciatic nerve ligation. Both pregabalin and baclofen were effective in reversing the hyperalgesia, but pregabalin was the more effective of the two drugs at all time points.

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