Influence of oriented nanofibrous PCL scaffolds on quantitative gene expression during neural differentiation of mouse embryonic stem cells

2015 ◽  
Vol 104 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Naghmeh Abbasi ◽  
Seyed Mahmoud Hashemi ◽  
Mohammad Salehi ◽  
Hoda Jahani ◽  
Seyed Javad Mowla ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Quantitative Gene Expression

Alteration of gene expression profile in mouse embryonic stem cells and neural differentiation deficits by ethephon

2020 ◽  
Vol 39 (11) ◽  
pp. 1518-1527
Author(s):  
S Mohammadi Nejad ◽  
M Hodjat ◽  
SA Mousavi ◽  
M Baeeri ◽  
MA Rezvanfar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential

Ethephon, a member of the organophosphorus compounds, is one of the most widely used plant growth regulators for artificial ripening. Although million pounds of this chemical is being used annually, the knowledge regarding its molecular toxicity is yet not sufficient. The purpose of this study was to evaluate the potential developmental toxicity of ethephon using embryonic stem cell model. The mouse embryonic stem cells (mESCs) were exposed to various concentrations of ethephon and the viability, cell cycle alteration and changes in the gene expression profile were evaluated using high-throughput RNA sequencing. Further, the effect of ethephon on neural differentiation potential was examined. The results showed that ethephon at noncytotoxic doses induced cell cycle arrest in mESCs. Gene ontology enrichment analysis showed that terms related to cell fate and organismal development, including neuron fate commitment, embryo development and cardiac cell differentiation, were markedly enriched in ethephon-treated cells. Neural induction of mESCs in the presence of ethephon was inhibited and the expression of neural genes was decreased in differentiated cells. Results obtained from this work clearly demonstrate that ethephon affects the gene expression profile of undifferentiated mESCs and prevents neural differentiation. Therefore, more caution against the frequent application of ethephon is advised.

scholarly journals The Trends in Global Gene Expression in Mouse Embryonic Stem Cells During Spaceflight

2019 ◽  
Vol 10 ◽  
Author(s):  
Lili An ◽  
Yanming Li ◽  
Yingjun Fan ◽  
Ning He ◽  
Fanlei Ran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Mouse Embryonic Stem Cells

Influence of random and oriented electrospun fibrous poly(lactic-co -glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

2017 ◽  
Vol 105 (5) ◽  
pp. 1333-1345 ◽  
Author(s):  
Laura E. Sperling ◽  
Karina P. Reis ◽  
Laura G. Pozzobon ◽  
Carolina S. Girardi ◽  
Patricia Pranke
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Glycolic Acid ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

3076 – FINE-TUNING GENE EXPRESSION BY CRISPRA IN MOUSE EMBRYONIC STEM CELLS FOR SPECIFIC TISSUE INDUCTION

2020 ◽  
Vol 88 ◽  
pp. S62
Author(s):  
Luis Galán Palma ◽  
Roshana Thambyrajah ◽  
Antonella Fidanza ◽  
Lesley Forrester ◽  
Pablo Menéndez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Fine Tuning ◽  
Specific Tissue

Asxl1 ablation in mouse embryonic stem cells impairs neural differentiation without affecting self-renewal

2019 ◽  
Vol 508 (3) ◽  
pp. 907-913 ◽  
Author(s):  
SoJung An ◽  
Ui-Hyun Park ◽  
Seungtae Moon ◽  
Myengmo Kang ◽  
Hyesook Youn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Self Renewal

scholarly journals RGD-Functionalized Nanofibers Increase Early GFAP Expression during Neural Differentiation of Mouse Embryonic Stem Cells

2019 ◽  
Vol 20 (3) ◽  
pp. 1443-1454 ◽  
Author(s):  
Diana L. Philip ◽  
Elena A. Silantyeva ◽  
Matthew L. Becker ◽  
Rebecca K. Willits
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gfap Expression

Embryoid Body-Mediated Differentiation of Mouse Embryonic Stem Cells Along a Hepatocyte Lineage: Insights from Gene Expression Profiles

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730040
Author(s):  
Eric I. Novik ◽  
Tim J. Maguire ◽  
Ksenia Orlova ◽  
Rene S. Schloss ◽  
Martin L. Yarmush
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryoid Body ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Gene Expression Profiles ◽  
Mouse Embryonic Stem Cells
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