Synthesis and characterization of thiol‐acrylate hydrogels using a base‐catalyzed Michael addition for 3D cell culture applications

2020 ◽  
Vol 108 (5) ◽  
pp. 2294-2307 ◽  
Author(s):  
Anowar H. Khan ◽  
Jeffery K. Cook ◽  
Wayne J. Wortmann ◽  
Nathan D. Kersker ◽  
Asha Rao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Michael Addition ◽  
3D Cell Culture ◽  
Synthesis And Characterization

Fabrication and characterization of egg white cryogel scaffold for three-dimensional (3D) cell culture

2019 ◽  
Vol 17 ◽  
pp. 441-446 ◽  
Author(s):  
Perumalsamy Balaji ◽  
Anbazhagan Murugadas ◽  
Sellathamby Shanmugaapriya ◽  
Mohammad Abdulkader Akbarsha
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Egg White ◽  
Fabrication And Characterization

scholarly journals Synthesis and characterization of poly(amino acid methacrylate)-stabilized diblock copolymer nano-objects

2015 ◽  
Vol 6 (10) ◽  
pp. 1805-1816 ◽  
Author(s):  
Vincent Ladmiral ◽  
Alexandre Charlot ◽  
Mona Semsarilar ◽  
Steven. P. Armes
Keyword(s):  
Amino Acid ◽  
Diblock Copolymer ◽  
Michael Addition ◽  
Self Assembly ◽  
Building Blocks ◽  
Synthesis And Characterization

Two amino acid methacrylates prepared via Michael addition are used as building blocks to prepare novel diblock copolymer nano-objects via polymerisation-induced self-assembly.

Preparation and Characterization of Thermoresponsive PEG-Based Injectable Hydrogels and Their Application for 3D Cell Culture

2020 ◽  
Vol 21 (3) ◽  
pp. 1254-1263
Author(s):  
Tian Li ◽  
Fei Huang ◽  
Diana Diaz-Dussan ◽  
Jianyang Zhao ◽  
Shruti Srinivas ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Injectable Hydrogels

scholarly journals Spatiotemporally Controllable and Cytocompatible Approach Builds 3D Cell Culture Matrix by Photo-Uncaged-Thiol Michael Addition Reaction

2014 ◽  
Vol 26 (29) ◽  
pp. 4919-4919 ◽  
Author(s):  
Zhenzhen Liu ◽  
Qiuning Lin ◽  
Yun Sun ◽  
Tao Liu ◽  
Chunyan Bao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Michael Addition ◽  
Addition Reaction ◽  
3D Cell Culture ◽  
Michael Addition Reaction

scholarly journals Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture

2017 ◽  
Vol 4 (4) ◽  
pp. 51 ◽  
Author(s):  
Dominik Egger ◽  
Monica Fischer ◽  
Andreas Clementi ◽  
Volker Ribitsch ◽  
Jan Hansmann ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Perfusion Bioreactor

scholarly journals Electrophilic oligodeoxynucleotide synthesis using dM-Dmoc for amino protection

2019 ◽  
Vol 15 ◽  
pp. 1116-1128 ◽  
Author(s):  
Shahien Shahsavari ◽  
Dhananjani N A M Eriyagama ◽  
Bhaskar Halami ◽  
Vagarshak Begoyan ◽  
Marina Tanasova ◽  
...  
Keyword(s):  
Michael Addition ◽  
Steric Hindrance ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Side Product ◽  
Synthesis And Characterization ◽  
Protecting Group ◽  
Phase Synthesis ◽  
Improved Technology

Solid-phase synthesis of electrophilic oligodeoxynucleotides (ODNs) was achieved using dimethyl-Dmoc (dM-Dmoc) as amino protecting group. Due to the high steric hindrance of the 2-(propan-2-ylidene)-1,3-dithiane side product from deprotection, the use of excess nucleophilic scavengers such as aniline to prevent Michael addition of the side product to the deprotected ODN during ODN cleavage and deprotection was no longer needed. The improved technology was demonstrated by the synthesis and characterization of five ODNs including three modified ones. The modified ODNs contained the electrophilic groups ethyl ester, α-chloroamide, and thioester. Using the technology, the sensitive groups can be installed at any location within the ODN sequences without using any sequence- or functionality-specific conditions and procedures.

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