Osteogenic potential and properties of injectable silk fibroin/tetracalcium phosphate/monetite composite powder biocement systems

2021 ◽  
Author(s):  
Lubomir Medvecky ◽  
Maria Giretova ◽  
Radoslava Stulajterova ◽  
Lenka Luptakova ◽  
Tibor Sopcak ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Composite Powder ◽  
Osteogenic Potential ◽  
Tetracalcium Phosphate

Enhanced osteogenic potential of human mesenchymal stem cells on electrospun nanofibrous scaffolds prepared from eri-tasar silk fibroin

2014 ◽  
Vol 103 (5) ◽  
pp. 971-982 ◽  
Author(s):  
Niladri nath Panda ◽  
Amit Biswas ◽  
Krishna Pramanik ◽  
Sriramakamal Jonnalagadda
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Nanofibrous Scaffolds

Effects of Liquid-to-Powder Ratio on the Properties of Self-Setting Silk Fibroin/Calcium Phosphate Composites

2012 ◽  
Vol 550-553 ◽  
pp. 1133-1138 ◽  
Author(s):  
Rui Juan Xie ◽  
Yang Yang Huang ◽  
Biao Wang ◽  
Meng Zhang
Keyword(s):  
Compressive Strength ◽  
Calcium Phosphate ◽  
Silk Fibroin ◽  
Significant Influence ◽  
High Purity ◽  
Setting Time ◽  
Obvious Effect ◽  
Tetracalcium Phosphate ◽  
Cooling Method

In this study, the high purity tetracalcium phosphate (TTCP) was prepared conveniently after studying the effect of cooling method on the purity of TTCP. The influence of liquid-to-powder (L/P) ratio on the properties of self-setting silk fibroin/calcium phosphate composites was studied. The results showed that the temperature of the furnace had a significant influence on the purity of TTCP when samples were removed from the furnace. Whether using N2 or not had no obvious effect on the purity of TTCP when the temperature declined from 1500°C to 1300°C. The setting time of composites became longer with the increase of L/P ratio in a range 0.32 - 0.39 ml/g, but all less than 15min. The injectability of the composites improved significantly as the L/P ratio increased. The compressive strength of composites reached maximum at the L/P ratio of 0.34 ml/g. The compressive strength decreased with the increase of L/P ratio greater than 0.34ml/g. The L/P ratio had no significant effect on the structure of the composites and the rod-like crystal of hydroxyapatite appeared in all the hardening-body of composites.

Understanding the molecular mechanism of improved proliferation and osteogenic potential of human mesenchymal stem cells grown on a polyelectrolyte complex derived from non-mulberry silk fibroin and chitosan

2017 ◽  
Vol 13 (1) ◽  
pp. 015011 ◽  
Author(s):  
Akalabya Bissoyi ◽  
Abhishek Kumar Singh ◽  
Subrat Kumar Pattanayak ◽  
Arindam Bit ◽  
Sudip Kumar Sinha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Molecular Mechanism ◽  
Silk Fibroin ◽  
Polyelectrolyte Complex ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential

Osteogenic potential of Rosuvastatin immobilized on silk fibroin nanofibers using argon plasma treatment

2018 ◽  
Vol 14 (2) ◽  
pp. 025002 ◽  
Author(s):  
Mohammad Mehdi Kalani ◽  
Jhamak Nourmohammadi ◽  
Babak Negahdari
Keyword(s):  
Plasma Treatment ◽  
Silk Fibroin ◽  
Argon Plasma ◽  
Osteogenic Potential ◽  
Silk Fibroin Nanofibers

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

2017 ◽  
Vol 204 (3-4) ◽  
pp. 150-163 ◽  
Author(s):  
S. Pina ◽  
R.F. Canadas ◽  
G. Jiménez ◽  
M. Perán ◽  
J.A. Marchal ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Calcium Phosphates ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential

The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Here, we present novel biofunctional scaffolds for bone regeneration composed of silk fibroin (SF) and β-tricalcium phosphate (β-TCP) and incorporating Sr, Zn, and Mn, which were successfully developed using salt-leaching followed by a freeze-drying technique. The scaffolds presented a suitable pore size, porosity, and high interconnectivity, adequate for promoting cell attachment and proliferation. The degradation behavior and compressive mechanical strengths showed that SF/ionic-doped TCP scaffolds exhibit improved characteristics for bone tissue engineering when compared with SF scaffolds alone. The in vitro bioactivity assays using a simulated body fluid showed the growth of an apatite layer. Furthermore, in vitro assays using human adipose-derived stem cells presented different effects on cell proliferation/differentiation when varying the doping agents in the biofunctional scaffolds. The incorporation of Zn into the scaffolds led to improved proliferation, while the Sr- and Mn-doped scaffolds presented higher osteogenic potential as demonstrated by DNA quantification and alkaline phosphatase activity. The combination of Sr with Zn led to an influence on cell proliferation and osteogenesis when compared with single ions. Our results indicate that biofunctional ionic-doped composite scaffolds are good candidates for further in vivo studies on bone tissue regeneration.

The effects of tetracycline-loaded silk fibroin membrane on proliferation and osteogenic potential of mesenchymal stem cells

2014 ◽  
Vol 192 (2) ◽  
pp. e1-e9 ◽  
Author(s):  
Seong-Ho Jin ◽  
HaeYong Kweon ◽  
Jun-Beom Park ◽  
Chang-Hyen Kim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Osteogenic Potential

In vitro effect of prolactin on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Effect

Effect of lactation on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2014 ◽  
Author(s):  
Reis Amanda Maria Sena ◽  
Freitas Silva Juneo de ◽  
Silvia Silva Santos ◽  
Rogeria Serakides ◽  
Melo Ocarino Natalia de
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells
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