Green Fluorescent Protein variants fold differentially in prokaryotic and eukaryotic cells

2001 ◽  
Vol 81 (S36) ◽  
pp. 117-128 ◽  
Author(s):  
Andrea Sacchetti ◽  
Valeria Cappetti ◽  
Pierfrancesco Marra ◽  
Roberta Dell'Arciprete ◽  
Tarek El Sewedy ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Eukaryotic Cells ◽  
Green Fluorescent ◽  
Protein Variants

Motif trap: a rapid method to clone motifs that can target proteins to defined subcellular localisations

1999 ◽  
Vol 112 (23) ◽  
pp. 4207-4211
Author(s):  
L.A. Bejarano ◽  
C. Gonzalez
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Eukaryotic Cells ◽  
Subcellular Localisation ◽  
Dna Fragments ◽  
Rt Pcr ◽  
Rapid Procedure ◽  
Target Proteins ◽  
Protein Motifs ◽  
Green Fluorescent

We have developed a rapid procedure termed Motif Trap (MT) to identify protein motifs that are able to target proteins to a distinct subcellular localisation in eukaryotic cells. By expressing random DNA fragments fused to green fluorescent protein (GFP), individual cells with the GFP localisation of interest are readily isolated allowing for the expressed DNA fragments to be cloned by RT-PCR. These can then be used to identify the corresponding full-length cDNAs. Using MT, we have identified patterns of GFP localisation which correspond to every major organelle and compartment. We have shown that MT is useful to identify new sequences that determine subcellular localisation as well as known targeting motifs.

Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 3. Temperature Dependence of Proton Transfer†

Biochemistry ◽  
2005 ◽  
Vol 44 (24) ◽  
pp. 8701-8711 ◽  
Author(s):  
Tim B. McAnaney ◽  
Xinghua Shi ◽  
Paul Abbyad ◽  
Henry Jung ◽  
S. James Remington ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Temperature Dependence ◽  
Fluorescent Protein ◽  
Ph Sensors ◽  
Dual Emission ◽  
Green Fluorescent ◽  
Protein Variants

Structural and Spectral Response of Green Fluorescent Protein Variants to Changes in pH†,‡

Biochemistry ◽  
1999 ◽  
Vol 38 (17) ◽  
pp. 5296-5301 ◽  
Author(s):  
Marc-André Elsliger ◽  
Rebekka M. Wachter ◽  
George T. Hanson ◽  
Karen Kallio ◽  
S. James Remington
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Spectral Response ◽  
Green Fluorescent ◽  
Protein Variants
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