The relationship between calcium and the metabolism of plasma membrane phospholipids in hemolysis induced by brown spider venom phospholipase-D toxin

2011 ◽  
Vol 112 (9) ◽  
pp. 2529-2540 ◽  
Author(s):  
Daniele Chaves-Moreira ◽  
Fernanda N. Souza ◽  
Rosalvo T.H. Fogaça ◽  
Oldemir C. Mangili ◽  
Waldemiro Gremski ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Phospholipase D ◽  
Spider Venom ◽  
Membrane Phospholipids ◽  
Brown Spider ◽  
The Relationship

scholarly journals Modulation of membrane phospholipids, the cytosolic calcium influx and cell proliferation following treatment of B16-F10 cells with recombinant phospholipase-D from Loxosceles intermedia (brown spider) venom

Toxicon ◽  
2013 ◽  
Vol 67 ◽  
pp. 17-30 ◽  
Author(s):  
Ana Carolina Martins Wille ◽  
Daniele Chaves-Moreira ◽  
Dilza Trevisan-Silva ◽  
Mariana Gabriel Magnoni ◽  
Marianna Boia-Ferreira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Phospholipase D ◽  
Calcium Influx ◽  
Cytosolic Calcium ◽  
Spider Venom ◽  
Membrane Phospholipids ◽  
Brown Spider

scholarly journals Phospholipase D Toxins of Brown Spider Venom Convert Lysophosphatidylcholine and Sphingomyelin to Cyclic Phosphates

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72372 ◽  
Author(s):  
Daniel M. Lajoie ◽  
Pamela A. Zobel-Thropp ◽  
Vlad K. Kumirov ◽  
Vahe Bandarian ◽  
Greta J. Binford ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Spider Venom ◽  
Brown Spider ◽  
Cyclic Phosphates

Identification of a direct hemolytic effect dependent on the catalytic activity induced by phospholipase-D (dermonecrotic toxin) from brown spider venom

2009 ◽  
Vol 107 (4) ◽  
pp. 655-666 ◽  
Author(s):  
Daniele Chaves-Moreira ◽  
Olga M. Chaim ◽  
Youssef B. Sade ◽  
Kátia S. Paludo ◽  
Luiza H. Gremski ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Phospholipase D ◽  
Spider Venom ◽  
Dermonecrotic Toxin ◽  
Hemolytic Effect ◽  
Brown Spider

scholarly journals The BIR2/BIR3-interacting Phospholipase D gamma 1 negatively regulates immunity in Arabidopsis

2019 ◽  
Author(s):  
Maria A. Schlöffel ◽  
Andrea Salzer ◽  
Wei-Lin Wan ◽  
Ringo van Wijk ◽  
Maja Šemanjski ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Pseudomonas Syringae ◽  
Phospholipase D ◽  
Plant Immunity ◽  
Negative Regulator ◽  
Regulatory Function ◽  
Membrane Phospholipids ◽  
Effective Strategies ◽  
Pathogen Invasion ◽  
Molecular Patterns

ABSTRACTPlants have evolved effective strategies to defend themselves against pathogen invasion. Starting from the plasma membrane with the recognition of microbe-associated molecular patterns (MAMPs) via pattern recognition receptors, internal cellular signaling pathways are induced to ultimately fend off the attack. Phospholipase D (PLD) hydrolyzes membrane phospholipids to produce phosphatidic acid (PA), which has been proposed to play a second messenger role in immunity. The Arabidopsis PLD family consists of 12 members and for some a specific function in resistance towards a subset of pathogens has been shown. We demonstrate here that Arabidopsis PLDγ1, but not its close homologs PLDγ2 and PLDγ3, is specifically involved in plant immunity. Genetic inactivation of PLDγ1 resulted in increased resistance towards the virulent bacterium Pseudomonas syringae pv. tomato DC3000 and the necrotrophic fungus Botrytis cinerea. As pldγ1 mutant plants responded with elevated levels of reactive oxygen species to MAMP-treatment, a negative regulatory function for this PLD isoform is proposed. Importantly, PA levels in pldγ1 mutants were not affected compared to stressed wild-type plants, suggesting that alterations in PA levels are unlikely the cause for the enhanced immunity in the pldγ1 line. Instead, the plasma-membrane-attached PLDγ1 protein colocalized and associated with the receptor-like kinases BIR2 and BIR3, which are known negative regulators of pattern-triggered immunity. Moreover, complex formation of PLDγ1 and BIR2 was further promoted upon MAMP-treatment. Hence, we propose that PLDγ1 acts as a negative regulator of plant immune responses in complex with immunity-related proteins BIR2 and BIR3.One-sentence summaryA phospholipase D is a novel negative regulator of plant immunity and forms complexes with regulatory receptor-like kinases.

Potential Implications for Designing Drugs Against the Brown Spider Venom Phospholipase-D

2016 ◽  
Vol 118 (4) ◽  
pp. 726-738 ◽  
Author(s):  
Daniele Chaves-Moreira ◽  
Fábio Rogério de Moraes ◽  
Ícaro Putinhon Caruso ◽  
Olga Meiri Chaim ◽  
Andrea Senff-Ribeiro ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Spider Venom ◽  
Brown Spider

Biological and structural comparison of recombinant phospholipase D toxins from Loxosceles intermedia (brown spider) venom

Toxicon ◽  
2007 ◽  
Vol 50 (8) ◽  
pp. 1162-1174 ◽  
Author(s):  
Rodrigo Otávio S. Ribeiro ◽  
Olga Meiri Chaim ◽  
Rafael Bertoni da Silveira ◽  
Luiza Helena Gremski ◽  
Youssef Bacila Sade ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Spider Venom ◽  
Structural Comparison ◽  
Brown Spider

Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity

Biochimie ◽  
2008 ◽  
Vol 90 (11-12) ◽  
pp. 1722-1736 ◽  
Author(s):  
J. Kusma ◽  
O.M. Chaim ◽  
A.C.M. Wille ◽  
V.P. Ferrer ◽  
Y.B. Sade ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Phospholipase D ◽  
Spider Venom ◽  
Dermonecrotic Toxin ◽  
Brown Spider

scholarly journals Phospholipase-D activity and inflammatory response induced by brown spider dermonecrotic toxin: Endothelial cell membrane phospholipids as targets for toxicity

2011 ◽  
Vol 1811 (2) ◽  
pp. 84-96 ◽  
Author(s):  
Olga M. Chaim ◽  
Rafael B. da Silveira ◽  
Dilza Trevisan-Silva ◽  
Valéria P. Ferrer ◽  
Youssef B. Sade ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Membrane ◽  
Inflammatory Response ◽  
Phospholipase D ◽  
Membrane Phospholipids ◽  
Dermonecrotic Toxin ◽  
Brown Spider

scholarly journals Release of dialkylglycerol from purple membrane phospholipids by phospholipase D.

1990 ◽  
Vol 265 (11) ◽  
pp. 5956-5959
Author(s):  
A Muga ◽  
J L Arrondo ◽  
J I Gurtubay ◽  
F M Goñi
Keyword(s):  
Phospholipase D ◽  
Purple Membrane ◽  
Membrane Phospholipids

Interaction between cortisol and arachidonic acid on the secretion of LH from ovine pituitary tissue

1991 ◽  
Vol 131 (1) ◽  
pp. 87-94 ◽  
Author(s):  
A. W. Nangalama ◽  
G. P. Moberg
Keyword(s):  
Arachidonic Acid ◽  
Plasma Membrane ◽  
Suppressive Effect ◽  
Inhibitory Effect ◽  
Adrenal Steroids ◽  
Membrane Phospholipids ◽  
Pituitary Tissue ◽  
Receptor Sites ◽  
Lh Release ◽  
Gonadotrophin Releasing Hormone

ABSTRACT In several species, glucocorticoids act directly on the pituitary gonadotroph to suppress the gonadotrophin-releasing hormone (GnRH)-induced secretion of the gonadotrophins, especially LH. A mechanism for this action of these adrenal steroids has not been established, but it appears that the glucocorticoids influence LH release by acting on one or more post-receptor sites. This study investigated whether glucocorticoids disrupt GnRH-induced LH release by altering the liberation of arachidonic acid from plasma membrane phospholipids, a component of GnRH-induced LH release. Using perifused ovine pituitary tissue, it was established that exposure of gonadotrophs to 1–1000 nmol cortisol/l for 4 h or longer significantly reduced GnRH-stimulated LH release with the maximal inhibitory effect being observed after 6 h of exposure to cortisol. This suppressive effect of cortisol could be reversed by administration of arachidonic acid, which in its own right could stimulate LH release from ovine pituitary tissue. Furthermore, the inhibitory effect of cortisol on GnRH-stimulated LH release could be directly correlated with decreased pituitary responsiveness to GnRH-stimulated arachidonic acid liberation, consistent with our hypothesis that glucocorticoids can suppress GnRH-induced secretion of LH by reducing the amount of arachidonic acid available for the exocytotic response of GnRH. Journal of Endocrinology (1991) 131, 87–94

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