Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells

2012 ◽  
Vol 113 (2) ◽  
pp. 563-570 ◽  
Author(s):  
Hana Kotasová ◽  
Iva Veselá ◽  
Jan Kučera ◽  
Zbyněk Houdek ◽  
Jiřina Procházková ◽  
...  
2020 ◽  
Vol 21 (23) ◽  
pp. 8945
Author(s):  
Mercedes Fernández ◽  
Micaela Pannella ◽  
Vito Antonio Baldassarro ◽  
Alessandra Flagelli ◽  
Giuseppe Alastra ◽  
...  

While the role of thyroid hormones (THs) during fetal and postnatal life is well-established, their role at preimplantation and during blastocyst development remains unclear. In this study, we used an embryonic stem cell line isolated from rat (RESC) to study the effects of THs and retinoic acid (RA) on early embryonic development during the pre-implantation stage. The results showed that THs play an important role in the differentiation/maturation processes of cells obtained from embryoid bodies (EB), with thyroid hormone nuclear receptors (TR) (TRα and TRβ), metabolic enzymes (deiodinases 1, 2, 3) and membrane transporters (Monocarboxylate transporters -MCT- 8 and 10) being expressed throughout in vitro differentiation until the Embryoid body (EB) stage. Moreover, thyroid hormone receptor antagonist TR (1-850) impaired RA-induced neuroectodermal lineage specification. This effect was significantly higher when cells were treated with retinoic acid (RA) to induce neuroectodermal lineage, studied through the gene and protein expression of nestin, an undifferentiated progenitor marker from the neuroectoderm lineage, as established by nestin mRNA and protein regulation. These results demonstrate the contribution of the two nuclear receptors, TR and RA, to the process of neuroectoderm maturation of the in vitro model embryonic stem cells obtained from rat.


Gene ◽  
2017 ◽  
Vol 631 ◽  
pp. 54-67 ◽  
Author(s):  
Syed Mohmad Shah ◽  
Suresh Kumar Singla ◽  
Prabhat Palta ◽  
Radhey Sham Manik ◽  
Manmohan Singh Chauhan

2004 ◽  
Vol 28 (4) ◽  
pp. 229-238 ◽  
Author(s):  
Sung-Hye Park ◽  
Seong Hoe Park ◽  
Myeong-Cherl Kook ◽  
Eun-Young Kim ◽  
Sepill Park ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document