Transient Arrest of Germinal Vesicle Breakdown Improved In Vitro Development Potential of Buffalo (Bubalus Bubalis) Oocytes

2017 ◽  
Vol 119 (1) ◽  
pp. 278-289 ◽  
Author(s):  
Sandeep Kumar ◽  
Manish Kumar ◽  
Sunny Dholpuria ◽  
Parul Sarwalia ◽  
Vipul Batra ◽  
...  
Keyword(s):  
Germinal Vesicle ◽  
Bubalus Bubalis ◽  
Germinal Vesicle Breakdown ◽  
Development Potential ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals In vitro development of mouse fetal germ cells into mature oocytes

Reproduction ◽  
2007 ◽  
Vol 134 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Wei Shen ◽  
Lan Li ◽  
Zhaodai Bai ◽  
Qingjie Pan ◽  
Mingxiao Ding ◽  
...  
Keyword(s):  
Genomic Imprinting ◽  
Germ Cells ◽  
Follicular Development ◽  
Germinal Vesicle ◽  
Germinal Vesicle Breakdown ◽  
Primordial Follicles ◽  
Ovarian Cells ◽  
Fetal Mice ◽  
Vitro Development

Little is known about the mechanisms underlying primordial follicular formation and the acquisition of competence to resume meiosis by growing oocytes. It is therefore important to establish anin vitroexperimental model that allows one to study such mechanisms. Mouse follicular development has been studiedin vitroover the past several years; however, no evidence has been presented showing that mature oocytes can be obtained from mouse fetal germ cells prior to the formation of primordial follicles. In this study, a method has been established to obtain mature oocytes from the mouse fetal germ cells at 16.5 days postcoitum (dpc). From the initiation of primordial follicular formation to the growth of early secondary follicles, ovarian tissues from 16.5 dpc fetal mice were culturedin vitrofor 14 days. Subsequently, 678 intact secondary follicles were isolated from 182 mouse fetal ovaries and cultured for 12 days. A total of 141 oocytes inside antral follicles were maturedin vitro, and 102 oocytes underwent germinal vesicle breakdown. We found that 97 oocytes were fertilized and 15 embryos were able to form morula–blastocysts. We also analyzed various genomic imprinting markers and showed that the erasure of genomic imprinting markers in the parental generation was also imposed on the oocytes that developed from fetal germ cells. Our results demonstrate that mouse fetal germ cells are able to form primordial follicles with ovarian cells, and that oocytes within the growing follicles are able to mature normallyin vitro.

Roscovitine Treatment Improves Synchronization of Donor Cell Cycle in G0/G1 Stage and In Vitro Development of Handmade Cloned Buffalo (Bubalus bubalis) Embryos

2012 ◽  
Vol 14 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Mushariffa Muzaffer ◽  
G. Krishnakanth ◽  
Ambika P. Saha ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Donor Cell ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals In vitro development of human oocytes reconstructed by sequential transfer of germinal vesicle and MII spindle

2017 ◽  
Vol 108 (3) ◽  
pp. e57-e58 ◽  
Author(s):  
H. Liu ◽  
Z. Lu ◽  
M. Yang ◽  
Z. Liu ◽  
Z. Merhi ◽  
...  
Keyword(s):  
Germinal Vesicle ◽  
In Vitro Development ◽  
Human Oocytes ◽  
Vitro Development

Expression of nitric oxide synthase isoforms in different stages of buffalo (Bubalus bubalis) ovarian follicles: Effect of nitric oxide on in vitro development of preantral follicle

2012 ◽  
Vol 77 (2) ◽  
pp. 280-291 ◽  
Author(s):  
Pawan K. Dubey ◽  
Vrajesh Tripathi ◽  
Ram Pratap Singh ◽  
G. Saikumar ◽  
Amar Nath ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Bubalus Bubalis ◽  
Ovarian Follicles ◽  
Preantral Follicle ◽  
In Vitro Development ◽  
Oxide Synthase ◽  
Vitro Development

scholarly journals α-Tocopherol and l-ascorbic acid increase the in vitro development of IVM/IVF swamp buffalo (Bubalus bubalis) embryos

animal ◽  
2008 ◽  
Vol 2 (10) ◽  
pp. 1486-1490 ◽  
Author(s):  
K. Saikhun ◽  
T. Faisaikarm ◽  
Z. Ming ◽  
K.H. Lu ◽  
Y. Kitiyanant
Keyword(s):  
Ascorbic Acid ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Swamp Buffalo ◽  
Vitro Development

Effect of cryoprotectants and their concentration on in vitro development of vitrified-warmed immature oocytes in buffalo (Bubalus bubalis)

2004 ◽  
Vol 61 (5) ◽  
pp. 831-842 ◽  
Author(s):  
N.A Wani ◽  
S.N Maurya ◽  
A.K Misra ◽  
V.B Saxena ◽  
B.D Lakhchaura
Keyword(s):  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Immature Oocytes ◽  
Vitro Development

scholarly journals Supplementation of L-ascorbic acid improves the in vitro development of buffalo (Bubalus bubalis) embryos and alters the expression of apoptosis-related genes

2021 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Mayank Roshan ◽  
Diksha Dua ◽  
Ankur Sharma ◽  
Manish Tiwari ◽  
ManojKumar Singh ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level

2015 ◽  
Vol 17 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Himanshu Agrawal ◽  
Prabhat Palta ◽  
Manmohan S. Chauhan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Dna Methyltransferase ◽  
Bubalus Bubalis ◽  
Dna Methyltransferase 1 ◽  
In Vitro Development ◽  
Vitro Development ◽  
Methyltransferase 1

Treatment of donor cells with trichostatin A improves in vitro development and reprogramming of buffalo (Bubalus bubalis) nucleus transfer embryos

2013 ◽  
Vol 80 (8) ◽  
pp. 878-886 ◽  
Author(s):  
Chan Luo ◽  
Fenghua Lu ◽  
Xiaoli Wang ◽  
Zhiqiang Wang ◽  
Xiangping Li ◽  
...  
Keyword(s):  
Trichostatin A ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Donor Cells ◽  
Nucleus Transfer ◽  
Vitro Development

Ceratonia siliqua (Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism

2020 ◽  
Author(s):  
Azita Faramarzi ◽  
Farank Aghaz ◽  
Mitra Bakhtiari ◽  
Shiva Roshankhah ◽  
Zahra Rashidi ◽  
...  
Keyword(s):  
Germinal Vesicle ◽  
Ceratonia Siliqua ◽  
In Vitro Development ◽  
Vitro Development
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