Hypermethylation of IL‐10 gene is responsible for its low mRNA expression in Behçet's disease

2018 ◽  
Vol 119 (8) ◽  
pp. 6614-6622 ◽  
Author(s):  
Shahriar Alipour ◽  
Mohammad Nouri ◽  
Alireza Khabbazi ◽  
Nasser Samadi ◽  
Zohreh Babaloo ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Behcet's Disease

scholarly journals mRNA Expression Levels of NKp30, NKp46, NKG2D, Perforin and Granzyme in the Behçet’s Disease

2021 ◽  
Author(s):  
Metin Yusuf Gelmez ◽  
Suzan Çınar ◽  
Esin Aktaş ◽  
Fatma Betül Öktelik ◽  
Goncagul Babuna-kobaner ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Behcet's Disease ◽  
Expression Levels ◽  
Mrna Expression Levels

scholarly journals mRNA Expression Level of Interleukin Genes in the Determining Phases of Behçet's Disease

2015 ◽  
Vol 27 (3) ◽  
pp. 291 ◽  
Author(s):  
Serpil Taheri ◽  
Murat Borlu ◽  
Cem Evereklioglu ◽  
Sevda Yesim Ozdemir ◽  
Yusuf Ozkul
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Expression Level ◽  
Mrna Expression Level ◽  
Behcet's Disease

scholarly journals Glucocorticoid sensitivity in Behçet's disease

2012 ◽  
Vol 1 (2) ◽  
pp. 103-111 ◽  
Author(s):  
R A M Quax ◽  
J A M van Laar ◽  
R van Heerebeek ◽  
K Greiner ◽  
E Ben-Chetrit ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Gene Polymorphisms ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Healthy Controls ◽  
Behcet's Disease ◽  
Expression Levels ◽  
Mrna Expression Levels

ObjectiveGlucocorticoid (GC) sensitivity is highly variable among individuals and has been associated with susceptibility to develop (auto-)inflammatory disorders. The purpose of the study was to assess GC sensitivity in Behçet's disease (BD) by studying the distribution of four GC receptor (GR) gene polymorphisms and by measuring in vitro cellular GC sensitivity.MethodsHealthy controls and patients with BD in three independent cohorts were genotyped for four functional GR gene polymorphisms. To gain insight into functional differences in in vitro GC sensitivity, 19 patients with BD were studied using two bioassays and a whole-cell dexamethasone-binding assay. Finally, mRNA expression levels of GR splice variants (GR-α and GR-β) were measured.ResultsHealthy controls and BD patients in the three separate cohorts had similar distributions of the four GR polymorphisms. The Bcll and 9β minor alleles frequency differed significantly between Caucasians and Mideast and Turkish individuals.At the functional level, a decreased in vitro cellular GC sensitivity was observed. GR number in peripheral blood mononuclear cells was higher in BD compared with controls. The ratio of GR-α/GR-β mRNA expression levels was significantly lower in BD.ConclusionsPolymorphisms in the GR gene are not associated with susceptibility to BD. However, in vitro cellular GC sensitivity is decreased in BD, possibly mediated by a relative higher expression of the dominant negative GR-β splice variant. This decreased in vitro GC sensitivity might play an as yet unidentified role in the pathophysiology of BD.

scholarly journals Association of genetic variations in PTPN2 and CD122 with ocular Behcet’s disease

2018 ◽  
Vol 102 (7) ◽  
pp. 996-1002 ◽  
Author(s):  
Qi Zhang ◽  
Hua Li ◽  
Shengping Hou ◽  
Hongsong Yu ◽  
Guannan Su ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Mononuclear Cells ◽  
Behçet's Disease ◽  
Genetic Variations ◽  
Janus Kinase ◽  
Behcet's Disease ◽  
Pathergy Test ◽  
Significant Difference

BackgroundProtein tyrosine phosphatases (PTPs) play critical roles in human autoimmunity. Previous studies found that PTPN2 may be the key regulatory factor in the T-cell-mediated immune response. PTPN2 regulates the Janus kinase/signal transducers and activators of transcription pathway by inhibiting signalling via the interleukin (IL)-2 receptor (CD122). An association between genetic variations in PTPN2 and CD122 with ocular Behcet’s disease (BD) has not yet been addressed and was therefore the purpose of this study.MethodsA two-stage case–control study was performed in 906 patients with ocular BD and 2178 healthy controls. Genotyping analysis of 11 single nucleotide polymorphisms was carried out. The expression of PTPN2 in peripheral blood mononuclear cells (PBMCs) was quantified by real-time PCR and cytokine production was measured by ELISA.ResultsThe frequency of the GG genotype of PTPN2-rs7234029 was significantly lower in patients with ocular BD (p=1.94×10−5, pc=8.34×10−4, OR=0.466). Stratification according to gender showed that rs7234029 was significantly associated with BD in men. A stratified analysis according to the main clinical features showed that rs7234029 was significantly associated with genital ulcers, skin lesions and a positive pathergy test. No association could be detected between BD and CD122 gene polymorphisms. Functional studies showed that rs7234029 GG genotype carriers had a higher PNPT2 mRNA expression level than those which carrying the AA or AG genotype, and a decreased secretion of IL-17 and tumour necrosis factor-alpha was seen by PBMCs from GG carriers. No significant difference could be detected concerning IL-1β or IL-6 production by stimulated PBMCs between the different genotype groups.ConclusionsThis study shows that a PTPN2-rs7234029 polymorphism is associated with ocular BD and is strongly influenced by gender. In addition, our results suggest that the genetic association with PTPN2 may involve the regulation of PTPN2 mRNA expression and cytokine secretion.

Interleukin-17 mRNA expression and serum levels in Behçet’s disease

Cytokine ◽  
2020 ◽  
Vol 127 ◽  
pp. 154994 ◽  
Author(s):  
Golamreza Jadideslam ◽  
Houman Kahroba ◽  
Khalil Ansarin ◽  
Ebrahim Sakhinia ◽  
Alireza Abhar ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Serum Levels ◽  
Interleukin 17 ◽  
Behcet's Disease

Coagulation and Fibrinolytic Activity in Behçet's Disease

1991 ◽  
Vol 66 (03) ◽  
pp. 292-294 ◽  
Author(s):  
K K Hampton ◽  
M A Chamberlain ◽  
D K Menon ◽  
J A Davies
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Behçet’S Disease ◽  
Fibrinolytic Activity ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Plasminogen Activator Inhibitor ◽  
Behcet's Disease ◽  
Tissue Plasminogen ◽  
Von Willebrand

SummaryCoagulation and fibrinolytic activities were studied in 18 subjects with Behçet's disease and compared with results from 14 matched control patients suffering from sero-negative arthritis. Significantly higher plasma concentrations (median and range) were found in Behçet's patients for the following variables: fibrinogen 3.7 (1.7-6.9) vs 3.0 (2.0-5.1) g/1, p <0.05; von Willebrand factor antigen, 115 (72-344) vs 74 (60-119)%, p <0.002; plasminogen activator activity (106/ECLT2) 219 (94-329) vs 137 (78-197) units, p <0.002; tissue plasminogen activator inhibitor (t-PA-I) activity, 9.1 (5.5-19.3) vs 5.1 (1.8-12.0) IU/ml, p <0.002; and PAI-1 antigen, 13.9 (4.5-20.9) vs 6.4 (2.4-11.1) ng/ml, p <0.002. Protein C antigen was significantly lower: 97 (70-183) vs 126 (96-220)%, p <0.02. No differences were observed in antithrombin III activity or antigen, factor VIII coagulant activity, fibrinopeptides A and Bβ15-42, plasminogen, α-2-antiplasmin, functional and immunological tissue-plasminogen activator, thrombin-antithrombin complexes and D-dimer. Levels of tissue plasminogen activator inhibitor (activity and antigen) correlated with disease activity while fibrinogen and von Willebrand factor concentrations did not. Seven of the 18 subjects with Behçet's disease had suffered thrombotic events but it was not possible to distinguish these from the 11 patients without thrombosis using the assays performed. The results suggest the abnormal fibrinolytic activity in Behçet's disease is due to increased inhibition of tissue plasminogen activator. No abnormality of coagulation or fibrinolytic activity specific to Behçet's disease was detected.

Circulating Thrombomodulin as a Clue of Endothelial Damage in Behçet’s Disease

1996 ◽  
Vol 75 (06) ◽  
pp. 974-975 ◽  
Author(s):  
İbrahim C Haznedaroğlu ◽  
Oktay Özdemir ◽  
Osman Özcebe ◽  
Semra V Dündar ◽  
Şerafettin Kirazli
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Endothelial Damage ◽  
Behcet's Disease

Radiological evaluation of vasculo-Behcet's disease

1993 ◽  
Vol 29 (1) ◽  
pp. 62 ◽  
Author(s):  
Kyung Hwan Lee ◽  
Jae Hyung Park ◽  
Joon Ku Han ◽  
Hyun Ae Park ◽  
Jin Wook Chung ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Radiological Evaluation ◽  
Behcet's Disease

Subacute thyroiditis in association with psoriasis and behcet's disease

2018 ◽  
Author(s):  
Ferreira Paulo Carvalho ◽  
Bruno Maia ◽  
Antonio Marinho ◽  
Ana Veloso ◽  
Ivo Cunha ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Subacute Thyroiditis ◽  
Behcet's Disease
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