Mapping protein expression in mouse pancreatic islets by immunolabeling and electron energy loss spectrum-imaging

2003 ◽  
Vol 61 (5) ◽  
pp. 448-456 ◽  
Author(s):  
Gertrud Goping ◽  
Harvey B. Pollard ◽  
Meera Srivastava ◽  
Richard Leapman
Keyword(s):  
Protein Expression ◽  
Energy Loss ◽  
Pancreatic Islets ◽  
Electron Energy ◽  
Electron Energy Loss Spectrum ◽  
Mouse Pancreatic Islets ◽  
Spectrum Imaging ◽  
Electron Energy Loss

Mapping the Subcellular Distribution of Calcium in Depolarized Neurons by Electron Energy Loss Spectrum Imaging

2000 ◽  
Vol 6 (S2) ◽  
pp. 162-163
Author(s):  
S.B. Andrews ◽  
J. Hongpaisan ◽  
N.B. Pivovarova ◽  
D.D. Friel ◽  
R.D. Leapman
Keyword(s):  
Electron Microscope ◽  
Energy Loss ◽  
Electron Energy ◽  
Transmission Electron Microscope ◽  
Detection Threshold ◽  
Electron Energy Loss Spectrum ◽  
Acquisition Time ◽  
Transmission Electron ◽  
Spectrum Imaging ◽  
Electron Energy Loss

In the context of biological specimens, it is in principle desirable to quantitatively map, rather than just point analyze, the distribution of physiologically important elements, and to do so at subcellular resolution. Presently, this can be accomplished by electron energy loss spectrum-imaging (EELSI) in both the scanning transmission electron microscope (STEM) and the energy-filtering transmission electron microscope (EFTEM). Until recently, this approach has been of limited value for mapping the particularly important element Ca, mainly because intracellular total Ca concentrations are normally quite low (<5 mmol/kg dry weight) and because the background in the vicinity of the Ca L23 edge is complex and requires precise background modeling to extract the very weak Ca signals. As a result, the Ca signal is usually not high enough to reach detection threshold during a practical EELSI acquisition time.

Electron energy-loss spectrum-imaging

1991 ◽  
Vol 38 (1) ◽  
pp. 47-73 ◽  
Author(s):  
J.A. Hunt ◽  
D.B. Williams
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectrum ◽  
Spectrum Imaging ◽  
Electron Energy Loss

Electron energy-loss spectrum imaging of high-k dielectric stacks

2006 ◽  
Vol 88 (2) ◽  
pp. 022108 ◽  
Author(s):  
M. MacKenzie ◽  
A. J. Craven ◽  
D. A. Hamilton ◽  
D. W. McComb
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectrum ◽  
High K ◽  
Spectrum Imaging ◽  
High K Dielectric ◽  
Electron Energy Loss

Applied Nanoanalysis by Electron Energy-Loss Spectrum Imaging Methods

2007 ◽  
Vol 13 (S03) ◽  
Author(s):  
B Schaffer ◽  
W Grogger ◽  
G Kothleitner ◽  
F Hofer
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectrum ◽  
Imaging Methods ◽  
Spectrum Imaging ◽  
Electron Energy Loss
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