Surface-replica cytochemistry as a tool for studying cell-surface enzyme activity: Membrane ecto-ATPase localization in liver cell cultures

1984 ◽  
Vol 1 (3) ◽  
pp. 289-298 ◽  
Author(s):  
Shuichi Karasaki ◽  
Akira Tanaka
Keyword(s):  
Enzyme Activity ◽  
Cell Surface ◽  
Cell Cultures ◽  
Liver Cell ◽  
Surface Replica ◽  
Atpase Localization

Oncornavirus assembly at the cell surface revealed in replicas of freeze-dried cells

1978 ◽  
Vol 36 (2) ◽  
pp. 354-355
Author(s):  
Anthony Demsey ◽  
Christopher W. Stackpole
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Viral Origin ◽  
Intact Cells ◽  
Structural Formation ◽  
Virus Core ◽  
Freeze Dried ◽  
Cacodylate Buffer ◽  
Surface Replica ◽  
Leukemia Viruses

The murine leukemia viruses are type-C oncornaviruses, and their release from the host cell involves a “budding” process in which the newly-forming, RNA-containing virus core becomes enveloped by modified cell surface membrane. Previous studies revealed that the released virions possess a dense array of 10 nm globular projections (“knobs”) on this envelope surface, and that these knobs contain a 70, 000 MW glycoprotein (gp70) of viral origin. Taking advantage of this distinctive structural formation, we have developed a procedure for freeze-drying and replication of intact cells which reveals surface detail superior to other surface replica techniques, and sufficient to detect even early stages of virus budding by localized aggregation of these knobs on the cell surface.Briefly, cells growing in monolayer are seeded onto round glass coverslips 10-12 mm in diameter. After a period of growth, cells are fixed in situ for one hour, usually with 1% OsO4 in 0. 1 M cacodylate buffer, and rinsed in distilled water.

Toxicity of 6-thioguanine and 8-azaguanine to non-dividing liver cell cultures

1985 ◽  
Vol 1 (2) ◽  
pp. 67-73 ◽  
Author(s):  
Jules J. Berman ◽  
Charles Tong ◽  
Gary M. Williams
Keyword(s):  
Cell Cultures ◽  
Liver Cell

Choline Deficiency, Lipid Peroxidation, Liver Cell Surface Receptor Alterations and Liver Tumor Promotion

1988 ◽  
pp. 591-599
Author(s):  
Hishashi Shinozuka ◽  
Chhanda Gupta ◽  
Atsuo Hattori ◽  
James M. Betschart ◽  
Mohamed A. Virji
Keyword(s):  
Lipid Peroxidation ◽  
Cell Surface ◽  
Liver Cell ◽  
Liver Tumor ◽  
Tumor Promotion ◽  
Cell Surface Receptor ◽  
Choline Deficiency ◽  
Surface Receptor

scholarly journals Production and release of infectious hepatitis C virus from human liver cell cultures in the three-dimensional radial-flow bioreactor

Virology ◽  
2003 ◽  
Vol 314 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Hideki Aizaki ◽  
Seishi Nagamori ◽  
Mami Matsuda ◽  
Hayato Kawakami ◽  
Osamu Hashimoto ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Cultures ◽  
Liver Cell ◽  
Human Liver ◽  
Radial Flow ◽  
Three Dimensional ◽  
Infectious Hepatitis ◽  
Human Liver Cell ◽  
Radial Flow Bioreactor

LACK OF TOXICITY OF HALOTHANE ON DIFFERENTIATED LIVER CELL CULTURES

1968 ◽  
Vol 12 (5) ◽  
pp. 464
Author(s):  
K. R. REES ◽  
A. J. ZUCKERMAN
Keyword(s):  
Cell Cultures ◽  
Liver Cell

Behavior and Pathogenicity of Tritrichomonas foetus in Chick Liver Cell Cultures*

1969 ◽  
Vol 16 (3) ◽  
pp. 479-495 ◽  
Author(s):  
JAROSLAV KULDA ◽  
B. M. HONIGBERG ◽  
Jelena Kulda
Keyword(s):  
Cell Cultures ◽  
Liver Cell ◽  
Tritrichomonas Foetus
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