Practical considerations in the use of polarized light microscopy in the analysis of the collagen network in articular cartilage

2008 ◽  
Vol 71 (4) ◽  
pp. 279-287 ◽  
Author(s):  
Jarno Rieppo ◽  
Jarmo Hallikainen ◽  
Jukka S. Jurvelin ◽  
Ilkka Kiviranta ◽  
Heikki J. Helminen ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Collagen Network

Structural Morphology of Rabbit Patella and Suprapatella Cartilage by Microscopic MRI and Polarized Light Microscopy

Cartilage ◽  
2021 ◽  
pp. 194760352199088
Author(s):  
Hannah Mantebea ◽  
Syeda Batool ◽  
Mouhamad Hammami ◽  
Yang Xia
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Design Method ◽  
Strong Dependence ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Structural Morphology ◽  
Pixel Resolution ◽  
Patellar Cartilage ◽  
Quantitative Results

Objective In order to appreciate the roles articular cartilage of sesamoid bones and sesamoid fibrocartilage play in anatomy and pathology, the articular cartilage of the patella ( n = 4) and suprapatella ( n = 4) (a sesamoid fibrocartilage) of 12 to 14 weeks old New Zealand rabbits were studied qualitatively and quantitatively. Design/Method The intact knee joints and block specimens from the joints were imaged using microscopic magnetic resonance imaging (µMRI) at a 97.6-µm pixel resolution for the former and 19.5-µm resolution for the latter. Histological sections were made out of the µMRI-imaged specimens, which were imaged using polarized light microscopy (PLM) at 0.25-, 1-, and 4-µm pixel resolutions. Results The patella cartilage varied in thickness across the medial to lateral ends of the sesamoid bone with the central medial aspect slightly thicker than the lateral aspect. The suprapatella fibrocartilage decreased proximally away from the knee joint. Quantitative results of patellar cartilage showed strong dependence of fiber orientation with the tissue depth. Three histological zones can be clearly observed, which are similar to articular cartilage from other large animals. The sesamoid fibrocartilage has one thin surface layer (10 µm thick) of parallel-arranged structured fibers followed immediately by the majority of random fibers in bulk tissue. T2 relaxation time anisotropy was observed in the patellar cartilage but not in the bulk fibrocartilage. Conclusion Given the different functions of these 2 different types of cartilages in joint motion, these quantitative results will be beneficial to future studies of joint diseases using rabbits as the animal model.

Collagenase-Induced Changes in Articular Cartilage as Detected by Electron-Microscopic Stereology, Quantitative Polarized Light Microscopy and Biochemical Assays

2002 ◽  
Vol 172 (4) ◽  
pp. 265-275 ◽  
Author(s):  
Teemu K. Långsjö ◽  
Jarno Rieppo ◽  
Alpo Pelttari ◽  
Niku Oksala ◽  
Vuokko Kovanen ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Electron Microscopic ◽  
Biochemical Assays ◽  
Induced Changes

Morphological Changes in Articular Cartilage Due to Static Compression: Polarized Light Microscopy Study

2007 ◽  
Vol 48 (2) ◽  
pp. 76-84 ◽  
Author(s):  
Hisham A. Alhadlaq ◽  
Yang Xia ◽  
Fay M. Hansen ◽  
Clifford M. Les ◽  
George Lust
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Morphological Changes ◽  
Polarized Light ◽  
Microscopy Study ◽  
Polarized Light Microscopy ◽  
Static Compression

Structural differences between immature and mature articular cartilage of rabbits by microscopic MRI and polarized light microscopy

2022 ◽  
Author(s):  
Hannah Mantebea ◽  
Syeda Batool ◽  
Amanveer Singh ◽  
Mohammed Hammami ◽  
Farid Badar ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Structural Differences
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