scholarly journals Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms

2021 ◽  
Vol 10 (6) ◽  
Author(s):  
Tanina Arab ◽  
Emily R. Mallick ◽  
Yiyao Huang ◽  
Liang Dong ◽  
Zhaohao Liao ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis

Characterization of aerosol particles during the most polluted season (winter) in urban Chengdu (China) by single-particle analysis

2019 ◽  
Vol 26 (17) ◽  
pp. 17685-17695 ◽  
Author(s):  
Jinqi Luo ◽  
Xiaojuan Huang ◽  
Junke Zhang ◽  
Bin Luo ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Single Particle ◽  
Aerosol Particles ◽  
Particle Analysis ◽  
Single Particle Analysis

Characterization of volcanic ash from the 2011 Grímsvötn eruption by means of single-particle analysis

2013 ◽  
Vol 79 ◽  
pp. 411-420 ◽  
Author(s):  
K.I. Lieke ◽  
T.B. Kristensen ◽  
U.S. Korsholm ◽  
J.H. Sørensen ◽  
K. Kandler ◽  
...  
Keyword(s):  
Single Particle ◽  
Volcanic Ash ◽  
Particle Analysis ◽  
Single Particle Analysis

scholarly journals Single particle analysis and stratification technology for extracellular vesicles

2021 ◽  
Vol 36 (2) ◽  
pp. 117-123
Author(s):  
Takanori Ichiki
Keyword(s):  
Extracellular Vesicles ◽  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis

scholarly journals Single particle analysis: Methods for detection of platelet extracellular vesicles in suspension (excluding flow cytometry)

Platelets ◽  
2016 ◽  
Vol 28 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Edit I. Buzás ◽  
Chris Gardiner ◽  
Changwon Lee ◽  
Zachary J. Smith
Keyword(s):  
Flow Cytometry ◽  
Extracellular Vesicles ◽  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Analysis Methods

Single-particle analysis of tear fluid reveals abundant presence of tissue factor-exposing extracellular vesicles with strong coagulation activity

Talanta ◽  
2021 ◽  
pp. 123089
Author(s):  
Haisheng Liu ◽  
Wenli Yuan ◽  
Qisheng Pang ◽  
Chengfeng Xue ◽  
Xiaomei Yan
Keyword(s):  
Tissue Factor ◽  
Extracellular Vesicles ◽  
Single Particle ◽  
Tear Fluid ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Coagulation Activity

Selection and characterization of aerosol particle size using a bessel beam optical trap for single particle analysis

2012 ◽  
Vol 14 (19) ◽  
pp. 6741 ◽  
Author(s):  
Antonia E. Carruthers ◽  
Jim S. Walker ◽  
Abby Casey ◽  
Andrew J. Orr-Ewing ◽  
Jonathan P. Reid
Keyword(s):  
Particle Size ◽  
Aerosol Particle ◽  
Single Particle ◽  
Bessel Beam ◽  
Optical Trap ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Aerosol Particle Size

scholarly journals Characterization of extracellular vesicles and artificial nanoparticles with four orthogonal single-particle analysis platforms

2020 ◽  
Author(s):  
Emily R. Mallick ◽  
Tanina Arab ◽  
Yiyao Huang ◽  
Liang Dong ◽  
Zhaohao Liao ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Single Particle ◽  
Size Exclusion ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Power Law Distribution ◽  
Polystyrene Spheres ◽  
Exclusion Chromatography ◽  
Human T Lymphocyte ◽  
Order Of Magnitude

ABSTRACTWe compared four orthogonal technologies for sizing, counting, and phenotyping of extracellular vesicles (EVs) and synthetic particles. The platforms were: single-particle interferometric reflectance imaging sensing (SP-IRIS) with fluorescence, nanoparticle tracking analysis (NTA) with fluorescence, microfluidic resistive pulse sensing (MRPS), and nanoflow cytometry measurement (NFCM). Results were compared with standard EV characterization techniques such as transmission electron microscopy (TEM) and Western blot (WB). EVs from the human T lymphocyte line H9 (high CD81, low CD63) and the promonocytic line U937 (low CD81, high CD63) were separated from culture conditioned medium (CCM) by differential ultracentrifugation (dUC) or a combination of ultrafiltration (UF) and size exclusion chromatography (SEC) and characterized per MISEV2018 guidelines. Mixtures of synthetic particles (silica and polystyrene spheres) with known sizes and/or concentrations were also tested. MRPS and NFCM returned similar particle counts, while NTA detected counts approximately one order of magnitude lower for EVs, but not for synthetic particles. SP-IRIS events could not be used to estimate particle concentrations. For sizing, SP-IRIS, MRPS, and NFCM returned similar size profiles, with smaller sizes predominating (per power law distribution), but with sensitivity typically dropping off below diameters of 60 nm. NTA detected a population of particles with a mode diameter greater than 100 nm. Additionally, SP-IRIS, MRPS, and NFCM were able to identify at least three of four distinct size populations in a mixture of silica or polystyrene nanoparticles. Finally, for tetraspanin phenotyping, the SP-IRIS platform in fluorescence mode and NFCM were able to detect at least two markers on the same particle. Based on the results of this study, we can draw conclusions about existing single-particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies.

Deposition of Ambient Particles in the Human Respiratory System Based on Single Particle Analysis: A Case Study in the Pearl River Delta, China

2021 ◽  
pp. 117056
Author(s):  
Shiguo Jia ◽  
Qi Zhang ◽  
Liming Yang ◽  
Sayantan Sarkar ◽  
Krishnan Padmaja ◽  
...  
Keyword(s):  
Respiratory System ◽  
Single Particle ◽  
Pearl River Delta ◽  
Pearl River ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Ambient Particles ◽  
The Pearl River Delta ◽  
The Pearl River

scholarly journals Thermo Scientific™ Tundra Cryo-TEM: 100kV Cryo-TEM dedicated for Single Particle Analysis

2021 ◽  
Vol 27 (S1) ◽  
pp. 1330-1332
Author(s):  
Zuzana Hlavenková ◽  
Dimple Karia ◽  
Miloš Malínský ◽  
Daniel Němeček ◽  
Fanis Grollios ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis
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