Histochemical identification and behavior of quail primordial germ cells injected into chick embryos by the intravascular route

1992 ◽  
Vol 261 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Masao Nakamura ◽  
Kazuya Yoshinaga ◽  
Toyoaki Fujimoto
1992 ◽  
Vol 16 (9) ◽  
pp. 853-857 ◽  
Author(s):  
I CHANG ◽  
A TAJIMA ◽  
Y YASUDA ◽  
T CHIKAMUNE ◽  
T OHNO

1974 ◽  
Vol 51 (5) ◽  
pp. 251-261 ◽  
Author(s):  
Masatoshi Fuyuta ◽  
Yukihiko Miyayama ◽  
Toyoaki Fujimoto

2008 ◽  
Vol 52 (4) ◽  
pp. 333-344 ◽  
Author(s):  
Christopher Runyan ◽  
Ying Gu ◽  
Amanda Shoemaker ◽  
Leendert Looijenga ◽  
Christopher Wylie

2003 ◽  
Vol 40 (2) ◽  
pp. 101-113 ◽  
Author(s):  
Dong-Feng Zhao ◽  
Hiroaki Yamashita ◽  
Masaharu Matsuzaki ◽  
Toshinori Takano ◽  
Shin-Ichi Abe ◽  
...  

Development ◽  
1978 ◽  
Vol 46 (1) ◽  
pp. 5-20
Author(s):  
H. Lee ◽  
N. Karasanyi ◽  
R. G. Nagele

Effects of concanavalin A (Con A) on the morphology and migration of primordial germ cells (PGCs) in stage-6 to -12 chick embryos were investigated. Con A, at a sublethal dose (10µg/ml), inhibited migration of PGCs from the germinal crescent area to other parts of the embryo. Affected PGCs were more rounded without the usual cytoplasmic extensions, but the integrity of other structures was unaffected. Nearly identical results were obtained with another lectin, wheat germ agglutinin (10µg/ml). Histochemistry using Con A-horseradish peroxidase revealed that PGCs in control embryos had a thin, rather uniform layer of extracellular coat material (ECM). Con A appeared to alter the distribution of ECM on PGCs, i.e. some parts of the cell surface were devoid of any detectable ECM, while others had small, scattered patches of ECM. Con A effects were alleviated by α-methyl-d-mannoside. Overall results of the present study indicated that the observed inhibition of PGC migration in early chick embryos is a consequence of Con A-induced alterations of cell surface properties.


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