Isolation and characterization of the cDNA encoding the spiny dogfish shark (Squalus acanthias) form of cytochrome P450c17

1995 ◽  
Vol 272 (1) ◽  
pp. 25-33 ◽  
Author(s):  
John M. Trant
Keyword(s):  
Squalus Acanthias ◽  
Spiny Dogfish ◽  
Isolation And Characterization ◽  
Dogfish Shark ◽  
Cytochrome P450c17

Characterization of an endothelin ET(B) receptor in the gill of the dogfish shark Squalus acanthias

1999 ◽  
Vol 202 (24) ◽  
pp. 3605-3610 ◽  
Author(s):  
D.H. Evans ◽  
M.P. Gunderson
Keyword(s):  
Blood Vessels ◽  
Gill Tissue ◽  
Squalus Acanthias ◽  
Single Site ◽  
Acid Base Balance ◽  
Ion Regulation ◽  
Base Balance ◽  
Dogfish Shark ◽  
Kinetics Of

Endothelins (ETs) are potent vasoconstrictive peptides that are secreted by the vascular endothelium and other tissues in vertebrates. Previous studies have demonstrated that ETs are expressed in a variety of fish tissues and contract various blood vessels. In order to determine if receptors for ET are expressed in fish gill tissue, we examined the binding kinetics of (125)I-labeled, human ET-1 to membrane fragments isolated from the gill of the dogfish shark, Squalus acanthias. (125)I-ET-1 bound at a single site, with a dissociation constant (K(d)) and binding site number (B(max)) very similar to those described in a variety of mammalian blood vessels. ET-1 and ET-3 competed equally with (125)I-ET-1, suggesting that the receptor was ET(B), which has been shown in mammalian systems to bind to both ligands equally. The ET(B)-specific agonists sarafotoxin S6c, IRL-1620, and BQ-3020 also competed against (125)I-ET-1 at a single site, supporting this hypothesis. We conclude that the shark gill expresses an ET(B) receptor with substantial homology to the mammalian receptor and that ET may play an important role in modulating such vital gill functions as gas exchange, ion regulation, acid-base balance, and excretion of nitrogen.

Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias)

2012 ◽  
Vol 36 (4) ◽  
pp. 665-679 ◽  
Author(s):  
Lauren E. Smith ◽  
Kathryn Crouch ◽  
Wei Cao ◽  
Mischa R. Müller ◽  
Leeying Wu ◽  
...  
Keyword(s):  
Squalus Acanthias ◽  
Spiny Dogfish ◽  
Immunoglobulin Repertoire

scholarly journals Multi-tissue RNA-seq and transcriptome characterisation of the spiny dogfish shark (Squalus acanthias) provides a molecular tool for biological research and reveals new genes involved in osmoregulation

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182756 ◽  
Author(s):  
Andres Chana-Munoz ◽  
Agnieszka Jendroszek ◽  
Malene Sønnichsen ◽  
Rune Kristiansen ◽  
Jan K. Jensen ◽  
...  
Keyword(s):  
Squalus Acanthias ◽  
Biological Research ◽  
Spiny Dogfish ◽  
Rna Seq ◽  
Molecular Tool ◽  
New Genes ◽  
Dogfish Shark
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