Group BStreptococcus(GBS) is the leading cause of neonatal invasive infections and an emerging pathogen in the elderly. Our objectives were to describe the evolution of GBS resistance to antibiotics in France and to investigate the emergence of fluoroquinolone (FQ) resistant isolates. A total of 8,757 unrelated GBS isolates were collected and tested for antibiotic susceptibility from 2007 and 2014 according to EUCAST recommendations. All isolates were susceptible to penicillin G, amoxicillin, and vancomycin. Resistance to macrolides decreased from 47.0% to 30.0% whereas high-level resistance to aminoglycosides, especially amikacin, increased from 6.4% to 8.8%, and 24 isolates (0.3%) were highly resistant to gentamicin. FQ resistance gradually increased from 0.2% in 2007 (n=1) to 1.5% in 2014 (n=18, p<0.01). Capsular (CPS) genotyping, Multi-Locus Sequence Typing, and sequencing of Quinolone Resistance Determining Region (QRDR) showed that GBS isolates of ST-19 CPS V were largely overrepresented in FQ resistant isolates (n=30, 45.5%). All thirty strains displayed the same QRDR mutations and were often associated with cross-resistance to macrolides (93.3%) and gentamicin (30%). In conclusion, we report the rise of FQ and aminoglycosides resistant GBS in France over an 8-year study period, an evolution likely linked to the clonal expansion of ST-19 CPS V resistant isolates. This study emphasizes the need for a continuous surveillance of GBS epidemiology and antibiotic susceptibility.