Oligonucleotide-mediated gene editing is underestimated in cells expressing mutated green fluorescent protein and is positively associated with target protein expression

2012 ◽  
Vol 14 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Petra Disterer ◽  
Ioannis Papaioannou ◽  
Vanessa C. Evans ◽  
J. Paul Simons ◽  
James S. Owen
Keyword(s):  
Green Fluorescent Protein ◽  
Protein Expression ◽  
Gene Editing ◽  
Fluorescent Protein ◽  
Target Protein ◽  
Green Fluorescent ◽  
In Cells

scholarly journals Stem/Progenitor Cell-Specific Enhanced Green Fluorescent Protein Expression Driven by the Endogenous Mcm2 Promoter

Stem Cells ◽  
2006 ◽  
Vol 25 (1) ◽  
pp. 132-138 ◽  
Author(s):  
Alexander Y. Maslov ◽  
Kimberly J. Bailey ◽  
Lawrence M. Mielnicki ◽  
Amy L. Freeland ◽  
Xiaolei Sun ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Protein Expression ◽  
Progenitor Cell ◽  
Enhanced Green Fluorescent Protein ◽  
Green Fluorescent Protein Expression ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals Effects of Recombinant Protein Expression on Green Fluorescent Protein Diffusion inEscherichia coli

Biochemistry ◽  
2009 ◽  
Vol 48 (23) ◽  
pp. 5083-5089 ◽  
Author(s):  
Kristin M. Slade ◽  
Rachael Baker ◽  
Michael Chua ◽  
Nancy L. Thompson ◽  
Gary J. Pielak
Keyword(s):  
Green Fluorescent Protein ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Fluorescent Protein ◽  
Recombinant Protein Expression ◽  
Inescherichia Coli ◽  
Protein Diffusion ◽  
Green Fluorescent

Imaging myosin 10 in cells

2004 ◽  
Vol 32 (5) ◽  
pp. 689-693 ◽  
Author(s):  
D. Tacon ◽  
P.J. Knight ◽  
M. Peckham
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Biology ◽  
Fluorescent Protein ◽  
Major Task ◽  
Green Fluorescent ◽  
In Cells

Cellular motors (kinesin, dynein and myosin) are ubiquitous. A major task in cell biology is to determine how they function in cells. Here we focus on myosin 10, an intrafilopodial motor, and show how imaging green fluorescent protein fused to myosin 10 or its tail domains can help us understand the function of this myosin.

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