Sustained tetracycline-regulated transgene expressionin vivo in rat retinal ganglion cells using a single type 2 adeno-associated viral vector

2003 ◽  
Vol 5 (6) ◽  
pp. 493-501 ◽  
Author(s):  
Sebastien Folliot ◽  
Delphine Briot ◽  
Herv� Conrath ◽  
Nathalie Provost ◽  
Yan Cherel ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Viral Vector ◽  
Ganglion Cells ◽  
Single Type ◽  
Retinal Ganglion

scholarly journals Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector

2005 ◽  
Vol 38 (3) ◽  
pp. 375-379 ◽  
Author(s):  
H. Petrs-Silva ◽  
V. Chiodo ◽  
L.B. Chiarini ◽  
W.W. Hauswirth ◽  
R. Linden
Keyword(s):  
Transcription Factor ◽  
Retinal Ganglion Cells ◽  
Viral Vector ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals The Olivary Pretectal Nucleus Receives Visual Input of High Spatial Resolution

2020 ◽  
Author(s):  
Jared N. Levine ◽  
Gregory W. Schwartz
Keyword(s):  
Single Cell ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Light Level ◽  
Single Type ◽  
Retinal Ganglion ◽  
Retinal Input ◽  
Wide Range ◽  
Pretectal Nucleus ◽  
Single Cell Type

AbstractIn the mouse, retinal output is computed by over 40 distinct types of retinal ganglion cells (RGCs) (Baden et al., 2016). Determining which of these many RGC types project to a retinorecipient region is a key step in elucidating the role that region plays in visually-mediated behaviors. Combining retrograde viral tracing and single-cell electrophysiology, we identify the RGC types which project to the olivary pretectal nucleus (OPN), a major visual structure. We find that retinal input to the OPN consists of a variety of intrinsically-photosensitive and conventional RGC types, the latter a diverse set of mostly ON RGCs. Surprisingly, while the OPN is most associated with the pupillary light reflex (PLR) pathway, requiring information about absolute luminance, we show that the majority of the retinal input to the OPN is from single cell type which transmits information unrelated to luminance. This ON-transient RGC accounts for two-thirds of the input to the OPN, and responds to small objects across a wide range of speeds. This finding suggests a role for the OPN in visually-mediated behaviors beyond the PLR.Significance statementThe olivary pretectal nucleus is a midbrain structure which receives direct input from retinal ganglion cells (RGC), and modulates pupil diameter in response to changing absolute light level. In the present study, we combine viral tracing and electrophysiology to identify the RGC types which project to the OPN. Surprisingly, the majority of its input comes from a single type which does not encode absolute luminance, but instead responds to small objects across a wide range of speeds. These findings are consistent with a role for the OPN apart from pupil control and suggest future experiments to elucidate its full role in visually-mediated behavior.

scholarly journals Multiplexed computations in retinal ganglion cells of a single type

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Stéphane Deny ◽  
Ulisse Ferrari ◽  
Emilie Macé ◽  
Pierre Yger ◽  
Romain Caplette ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Single Type ◽  
Retinal Ganglion

scholarly journals In vivo imaging of adeno-associated viral vector labelled retinal ganglion cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Corey A. Smith ◽  
Balwantray C. Chauhan
Keyword(s):  
Retinal Ganglion Cells ◽  
In Vivo Imaging ◽  
Viral Vector ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Differential role of two Ca(2+)-permeable non-NMDA glutamate channels in rat retinal ganglion cells: kainate-induced cytoplasmic and nuclear Ca2+ signals

1994 ◽  
Vol 72 (5) ◽  
pp. 2503-2516 ◽  
Author(s):  
T. Leinders-Zufall ◽  
M. N. Rand ◽  
S. G. Waxman ◽  
J. D. Kocsis
Keyword(s):  
Confocal Microscopy ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Divalent Cations ◽  
Neonatal Rat ◽  
Retinal Ganglion ◽  
Reversal Potentials ◽  
Retinal Slices

1. The permeability of non-N-methyl-D-aspartate (non-NMDA) glutamate channels to divalent cations and specifically the entry of Ca2+ and subsequent elevations in cytoplasmic and nuclear Ca2+ signals were investigated in cultured neonatal rat retinal ganglion cells using the whole cell patch-clamp technique and Ca2+ imaging with confocal microscopy. In addition, divalent-permeable non-NMDA receptor channels were studied in retinal slices using a Co2+ staining technique. 2. Using Ca2+ (2.5 mM) as the only permeable cation in the external solution, stimulation with 100 microM kainate produced nondesensitizing, nonselective cation currents with either low or high Ca2+ permeability. Both currents were reversibly blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Neurons with the low divalent-permeable currents (type 1) had reversal potentials of -41.5 +/- 4.4 mV (mean +/- SD), and neurons with the high divalent-permeable currents (type 2) had reversal potentials of -22.6 +/- 5.5 mV. The permeability ratio PCa/PCs was 3.3 for the type 1 currents and 8.5 for the type 2 currents, indicating a 2.5-fold greater permeability to Ca2+ for the type 2 non-NMDA glutamate channels. 3. Both types of non-NMDA glutamate channels showed relatively little selectivity between Ca2+ and Co2+. The type 1 neurons had a slightly higher permeability to Co2+ than to Ca2+, whereas the type 2 neurons were equally permeable to both divalent cations. The type 2 neurons had a much higher permeability for both divalent cations compared with the type 1 neurons. 4. Staining for Co2+ uptake through kainate-stimulated non-NMDA glutamate channels in retinal slices provided additional evidence for the presence of the two ganglion cell populations. Activation of the neurons by kainate in conditions isolating the non-NMDA glutamate channel caused differential uptake of Co2+. In contrast, depolarization in the presence of the non-NMDA antagonist CNQX failed to cause Co2+ influx. 5. Imaging experiments using confocal microscopy showed that kainate stimulation induced an increase in intracellular Ca2+ in both types of retinal ganglion cells, but only the type 2 neurons showed a substantial increase in cytoplasmic and nuclear Ca2+ signals. Kainate-induced Ca2+ signals in the type 2 neurons were almost nine times greater than those of the type 1 neurons. 6. When intracellular Ca2+ stores were depleted by brief treatment with thapsigargin, kainate-induced Ca2+ signals in the type 1 neurons were unchanged. However, in the type 2 neurons kainate no longer induced large Ca2+ signals in the cytoplasm and nucleus, despite normal influx of Ca2+.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Viral vector-mediated downregulation of RhoA increases survival and axonal regeneration of retinal ganglion cells

2014 ◽  
Vol 8 ◽  
Author(s):  
Jan Christoph Koch ◽  
Lars Tönges ◽  
Uwe Michel ◽  
Mathias Bähr ◽  
Paul Lingor
Keyword(s):  
Retinal Ganglion Cells ◽  
Axonal Regeneration ◽  
Viral Vector ◽  
Ganglion Cells ◽  
Retinal Ganglion

Distribution of glycine receptor subunits on primate retinal ganglion cells: a quantitative analysis

2000 ◽  
Vol 12 (12) ◽  
pp. 4155-4170 ◽  
Author(s):  
Bin Lin ◽  
Paul R. Martin ◽  
Samuel G. Solomon ◽  
Ulrike Grunert
Keyword(s):  
Quantitative Analysis ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Glycine Receptor ◽  
Retinal Ganglion

Calcium Signals Induced By Tonic Firing In Rat Retinal Ganglion Cells

2012 ◽  
Vol 3 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Kyril I. Kuznetsov ◽  
Vitaliy Yu. Maslov ◽  
Svetlana A. Fedulova ◽  
Nikolai S. Veselovsky
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Calcium Signals ◽  
Tonic Firing
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