Prostaglandin E and Thromboxane B2 Release by PMA-Induced U937 Cells in Response to C3b

1986 ◽  
Vol 39 (5) ◽  
pp. 511-519 ◽  
Author(s):  
Harvey A. Schenkein
Keyword(s):  
Thromboxane B2 ◽  
Prostaglandin E ◽  
U937 Cells

Isoprenaline inhibits thromboxane B2 release from U937 cells

1993 ◽  
Vol 53 (5) ◽  
pp. 559-562 ◽  
Author(s):  
C.E. Brightling ◽  
A.J. Baker ◽  
R.W. Fuller
Keyword(s):  
Thromboxane B2 ◽  
U937 Cells

Decreased systemic formation of prostaglandin E and prostacyclin, and unchanged thromboxane formation, in alcoholics during withdrawal as estimated from metabolites in urine

1987 ◽  
Vol 73 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Ulrich Förstermann ◽  
Thomas J. Feuerstein
Keyword(s):  
Hydrolysis Product ◽  
Thromboxane A2 ◽  
Normal Subjects ◽  
Thromboxane B2 ◽  
Urinary Metabolite ◽  
Prostaglandin E ◽  
Dioic Acid ◽  
Control Subjects ◽  
Major Urinary Metabolite ◽  
Prostaglandins E1 And E2

1. The rates of secretion into the circulation of prostaglandin E, prostacyclin, and thromboxane A2 were estimated in male alcoholics on the third day of withdrawal and in control subjects by measuring appropriate metabolites in urine. 2. Urinary levels of tetranor-5,11-diketo-7α-hydroxyprostane-1,16-dioic acid (the major urinary metabolite of prostaglandins E1 and E2), of 2,3-dinor-6-ketoprostaglandin F1α (the major urinary metabolite of prostacyclin) and of 6-keto-prostaglandin F1α (the stable hydrolysis product of prostacyclin) were significantly different from the normal subjects in the alcoholic group. In contrast, 2,3-dinor-thromboxane B2 (the major urinary metabolite of thromboxane A2) and thromboxane B2 (the stable hydrolysis product of thromboxane A2) were not significantly different between the groups. 3. These data suggest that the ratio of the vasodilator prostanoids prostaglandin E and prostacyclin and the vasoconstrictor prostanoid thromboxane A2 is lower than in normal subjects, in alcoholics during withdrawal. This may be one causal factor for the higher incidence of hypertension observed in withdrawing alcoholics compared with control subjects.

Cell-Bound C3b Stimulates Human Monocyte Release of Prostaglandin E and Thromboxane B2

1987 ◽  
Vol 41 (4) ◽  
pp. 363-366 ◽  
Author(s):  
David E. Fulford ◽  
R. Bruce Rutherford
Keyword(s):  
Human Monocyte ◽  
Thromboxane B2 ◽  
Prostaglandin E

PROSTAGLANDIN E, THROMBOXANE B2 AND 6-OXO-PROSTAGLANDIN F1α IN AMNIOTIC FLUID AND MATERNAL PLASMA OF RHESUS MONKEYS (MACACA MULATTA) DURING THE LATTER THIRD OF GESTATION

1979 ◽  
Vol 81 (3) ◽  
pp. 345-349 ◽  
Author(s):  
J. S. ROBINSON ◽  
R. NATALE ◽  
L. CLOVER ◽  
M. D. MITCHELL
Keyword(s):  
Rhesus Monkey ◽  
Amniotic Fluid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Maternal Plasma ◽  
Thromboxane B2 ◽  
Prostaglandin E ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
Serial Samples

The concentrations of prostaglandin E (PGE), thromboxane B2 (TXB2) and 6-oxo-prostaglandin F1α (6-oxo-PGF1α) were measured by radioimmunoassay in serial samples of amniotic fluid and maternal peripheral plasma in the latter third of pregnancy in rhesus monkeys (Macaca mulatta). The samples were collected under ketamine-induced anaesthesia. The concentration of PGE was undetectable in amniotic fluid until a few days before delivery when a large increase was observed in three of the five animals. There were small increases of TXB2 and 6-oxo-PGF1α in amniotic fluid before delivery. In maternal plasma the concentrations of PGE, TXB2 and 6-oxo-PGF1α were generally higher and more variable than in amniotic fluid and did not increase with advancing gestation. It is suggested that increased production of primary prostaglandins occurs before, and is involved in, the onset of parturition in the rhesus monkey.

scholarly journals Time Dependent Production of Cytokines and Eicosanoids by Human Monocytic Leukaemia U937 Cells; Effects of Glucocorticosteroids

1999 ◽  
Vol 8 (4-5) ◽  
pp. 229-235 ◽  
Author(s):  
Ingrid M. Garrelds ◽  
Peter Th. W. Van Hal ◽  
Raquel C. Haakmat ◽  
Henk C. Hoogsteden ◽  
Pramod R. Saxena ◽  
...  
Keyword(s):  
Fluticasone Propionate ◽  
Time Course ◽  
Mononuclear Cells ◽  
Interleukin 10 ◽  
Mononuclear Phagocytes ◽  
Prostaglandin E ◽  
U937 Cells ◽  
Necrosis Factor Alpha ◽  
Asthmatic Patients ◽  
Kinetics Of

In the present study the human monoblast cell line U937 has been used as a model to study the function of human mononuclear phagocytes in asthma. The kinetics of the production of eicosanoids and cytokines, which are thought to play a role in the pathogenesis of asthma, were studied. In addition, the effects of glucocorticosteroids were investigated, as these drugs are of great importance for the treatment of asthmatic patients. After stimulation with phorbol-12 myristate acetate (PMA) for 24h, U937 cells were cultured in the absence or presence of lipopolysaccharide (LPS: 1 and 5 μg ml-1) and glucocorticosteroids (budesonide, fluticasone propionate and prednisolone: 10-11, 10-9and 10-7M) for 96h. The production of interleukin- 1β (IL-1β), interleukin-6 (IL-6), prostaglandin E2(PGE2) and thromboxane B2(TxB2) gradually increased in time after stimulation with LPS, whereas the transient production of tumor necrosis factor alpha (TNF-α) reached its maximum between 6 and 12 h. Interferon-gamma (IFN-γ), interleukin-10 (IL-10) and leukotriene B4(LTB4) were not detectable. All three glucocorticosteroids (budesonide, fluticasone propionate and prednisolone) completely inhibited the production of both eicosanoids and cytokines. The production of eicosanoids was more sensitive to these glucocorticoids than the production of cytokines. The observed differences in the kinetics of the production of eicosanoids and cytokines stress the importance of time course experiments in studies on the effect of drugs on mononuclear cells.

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