High-affinity rat anti-fluorescein monoclonal antibody with unique fine specificity properties including differntail recognition of dynamic ligand analogues

1995 ◽  
Vol 8 (4) ◽  
pp. 258-269 ◽  
Author(s):  
Steven D. Miklasz ◽  
Gene A. Gulliver ◽  
Edward W. Voss
Keyword(s):  
Monoclonal Antibody ◽  
Fine Specificity ◽  
High Affinity

A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells

Blood ◽  
2004 ◽  
Vol 104 (8) ◽  
pp. 2540-2542 ◽  
Author(s):  
Mark S. Cragg ◽  
Mike B. Bayne ◽  
Alison L. Tutt ◽  
Ruth R. French ◽  
Stephen Beers ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Surface ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Lymphoma Cells ◽  
Fine Specificity ◽  
High Affinity ◽  
Anti Cd20

Abstract The chimeric anti-CD20 monoclonal antibody (mAb), rituximab, is an established part of the management of many non-Hodgkin lymphomas. The in vivo action of rituximab remains elusive, and this partially reflects a lack of highly specific reagents to detect rituximab binding at the cell surface. Here we report a new high-affinity mAb (MB2A4) with fine specificity for the idiotype of rituximab. It is able to detect rituximab in vitro, in the presence of high levels of human immunoglobulin G (IgG), in the serum of patients receiving rituximab therapy, and, surprisingly, when rituximab is bound to CD20 on the cell surface. We propose that the anti–idiotype (Id) binds to rituximab molecules bound univalently at the cell surface, facilitated by the relatively high off-rate of rituximab. This reagent provides new insights into the binding of rituximab at the cell surface and demonstrates a mode of binding that could be exploited for the surface detection of other mAbs with clinical and biologic applications.

Selection of aptamers with high affinity and high specificity against C595, an anti-MUC1 IgG3 monoclonal antibody, for antibody targeting

2005 ◽  
Vol 296 (1-2) ◽  
pp. 45-62 ◽  
Author(s):  
Sotiris Missailidis ◽  
Despina Thomaidou ◽  
K. Eszter Borbas ◽  
Mike R. Price
Keyword(s):  
Monoclonal Antibody ◽  
High Specificity ◽  
High Affinity ◽  
Antibody Targeting ◽  
Selection Of

scholarly journals Rolling on E- or P-selectin induces the extended but not high-affinity conformation of LFA-1 in neutrophils

Blood ◽  
2010 ◽  
Vol 116 (4) ◽  
pp. 617-624 ◽  
Author(s):  
Yoshihiro Kuwano ◽  
Oliver Spelten ◽  
Hong Zhang ◽  
Klaus Ley ◽  
Alexander Zarbock
Keyword(s):  
Monoclonal Antibody ◽  
Tyrosine Kinase ◽  
Binding Assay ◽  
P38 Map Kinase ◽  
Intercellular Adhesion Molecule ◽  
Reporter Assay ◽  
Rolling Velocity ◽  
High Affinity ◽  
Velocity Reduction ◽  
Blood Neutrophils

Abstract Human blood neutrophils rolling on E- or P-selectin reduced their rolling velocity when intercellular adhesion molecule (ICAM)–1 was available. Similar to mouse neutrophils, this was dependent on P-selectin glycoprotein ligand 1 (PSGL1), αLβ2 integrin, the Src family tyrosine kinase FGR and spleen tyrosine kinase SYK. Blocking phospholipase C or p38 MAP kinase attenuated, but did not abolish the velocity reduction. To test expression of integrin activation epitopes, we adapted an immobilized reporter assay and developed a new homogeneous microfluidics-based reporter antibody binding assay. Rolling on E- or P-selectin induced the extension reporter epitopes KIM127 and NKI-L16, but not the high affinity reporter epitope monoclonal antibody (mAb) 24. This enabled rolling neutrophils to bind to immobilized extension reporter, but not activation reporter antibodies and allowed binding of soluble KIM127 during rolling. We conclude that human neutrophil rolling on E- or P-selectin induces the extended αLβ2 integrin conformation through signaling triggered by PSGL-1 engagement.

scholarly journals Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors

mAbs ◽  
2017 ◽  
Vol 10 (1) ◽  
pp. 104-117 ◽  
Author(s):  
Caroline S. Colley ◽  
Bojana Popovic ◽  
Sudharsan Sridharan ◽  
Judit E. Debreczeni ◽  
David Hargeaves ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
High Affinity

Development of XPA067.06, a potent high affinity human anti-gastrin monoclonal antibody

2008 ◽  
Vol 76 (3) ◽  
pp. 340-352
Author(s):  
Ssucheng J. Hsu ◽  
Amita Patel ◽  
Paul D. Larsen ◽  
David J. Bohmann ◽  
Robert J. Bauer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
High Affinity

scholarly journals G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kasumi Tatsumi ◽  
Gyosuke Sakashita ◽  
Yuko Nariai ◽  
Kosuke Okazaki ◽  
Hiroaki Kato ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Epitope Tag ◽  
Soluble Peptide ◽  
High Affinity ◽  
System A
Sign in / Sign up

Export Citation Format

Share Document