Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

2009 ◽  
Vol 81 (20) ◽  
pp. 8441-8446 ◽  
Author(s):  
Yongcan Wang ◽  
Xu Zhu ◽  
Minghua Wu ◽  
Ning Xia ◽  
Jianxiu Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mutant P53 ◽  
Label Free ◽  
Wild Type

SPRI biosensors for quantitative determination of matrix metalloproteinase-2

2017 ◽  
Vol 9 (16) ◽  
pp. 2407-2414 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Iosif Sveklo ◽  
Ewa Matuszczak ◽  
Adam Hermanowicz ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Matrix Metalloproteinase ◽  
Quantitative Determination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Matrix Metalloproteinase 2 ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

The aim of this study was to develop a new, label-free, highly selective Surface Plasmon Resonance Imaging biosensor for the quantitative determination of matrix metalloproteinase-2.

Determination of kinetic constants for the interaction between a monoclonal antibody and peptides using surface plasmon resonance

Biochemistry ◽  
1992 ◽  
Vol 31 (27) ◽  
pp. 6298-6304 ◽  
Author(s):  
Daniele Altschuh ◽  
Marie Christine Dubs ◽  
Etienne Weiss ◽  
Gabrielle Zeder-Lutz ◽  
Marc H. V. Van Regenmortel
Keyword(s):  
Monoclonal Antibody ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Kinetic Constants

scholarly journals A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

2019 ◽  
Vol 20 (9) ◽  
pp. 2166 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Anna Sankiewicz ◽  
Adam Hermanowicz ◽  
Krzysztof Sobolewski ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Blood Plasma ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cathepsin L ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Standard Curve

The purpose of this study was to develop a new method for a determination of the cathepsin L—biosensor based on the Surface Plasmon Resonance Imaging technique. The cathepsin L is an endopeptidase, which degrades proteins and plays an important role in various processes occurring in the human body. The detection technique, Surface Plasmon Resonance Imaging, is an optical, label-free technique, which can be used for quantitative determination of the different proteins. In order to bind the enzyme, the cathepsin L inhibitor—RKLLW-NH2 was used. The validation process showed that parameters: precision, accuracy, and selectivity of the method were acceptable. The analytically useful range of the standard curve was 0.50 ng/mL—15.00 ng/mL. The detection and quantification limit of method was 1.67 pg/mL and 5.07 pg/mL, respectively. The usefulness of the developed method was confirmed by the determination of the cathepsin L concentration in the blood plasma of some healthy persons and in the blood plasma of patients. The obtained results were compared with the results obtained by the ELISA. It was found that the correlation between these two methods was very strong, what suggest that the developed method can be used as the competitive method to the ELISA.

scholarly journals Optical Characterization of Ultra-Thin Films of Azo-Dye-Doped Polymers Using Ellipsometry and Surface Plasmon Resonance Spectroscopy

Photonics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 41
Author(s):  
Najat Andam ◽  
Siham Refki ◽  
Hidekazu Ishitobi ◽  
Yasushi Inouye ◽  
Zouheir Sekkat
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Spectroscopic Ellipsometry ◽  
Plasmon Resonance ◽  
Complex Refractive Index ◽  
Resonance Spectroscopy ◽  
Doped Polymers

The determination of optical constants (i.e., real and imaginary parts of the complex refractive index (nc) and thickness (d)) of ultrathin films is often required in photonics. It may be done by using, for example, surface plasmon resonance (SPR) spectroscopy combined with either profilometry or atomic force microscopy (AFM). SPR yields the optical thickness (i.e., the product of nc and d) of the film, while profilometry and AFM yield its thickness, thereby allowing for the separate determination of nc and d. In this paper, we use SPR and profilometry to determine the complex refractive index of very thin (i.e., 58 nm) films of dye-doped polymers at different dye/polymer concentrations (a feature which constitutes the originality of this work), and we compare the SPR results with those obtained by using spectroscopic ellipsometry measurements performed on the same samples. To determine the optical properties of our film samples by ellipsometry, we used, for the theoretical fits to experimental data, Bruggeman’s effective medium model for the dye/polymer, assumed as a composite material, and the Lorentz model for dye absorption. We found an excellent agreement between the results obtained by SPR and ellipsometry, confirming that SPR is appropriate for measuring the optical properties of very thin coatings at a single light frequency, given that it is simpler in operation and data analysis than spectroscopic ellipsometry.

Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Localized Surface Plasmon ◽  
Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

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