Molecular epidemiology of enterovirus outbreaks in Canada during 1991–1992: Identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing

1994 ◽  
Vol 44 (4) ◽  
pp. 340-347 ◽  
Author(s):  
Michael A. Drebot ◽  
Christopher Y. Nguan ◽  
Janice J. Campbell ◽  
Spencer H. S. Lee ◽  
Kevin R. Forward
Keyword(s):  
Molecular Epidemiology ◽  
Amplicon Sequencing ◽  
Echovirus 30 ◽  
Coxsackievirus B1

scholarly journals Molecular Epidemiology and Evolutionary Trajectory of Emerging Echovirus 30, Europe

2021 ◽  
Vol 27 (6) ◽  
pp. 1616-1626
Author(s):  
Kimberley S.M. Benschop ◽  
Eeva K. Broberg ◽  
Emma Hodcroft ◽  
Dennis Schmitz ◽  
Jan Albert ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Evolutionary Trajectory ◽  
Echovirus 30

Molecular epidemiology of Echovirus 30 in Taiwan, 1988–2008

Virus Genes ◽  
2011 ◽  
Vol 42 (2) ◽  
pp. 178-188 ◽  
Author(s):  
Guan-Ming Ke ◽  
Kuei-Hsiang Lin ◽  
Po-Liang Lu ◽  
Yi-Chin Tung ◽  
Chu-Feng Wang ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Echovirus 30

Molecular Epidemiology and Prevalence of Echovirus 30 in Zhejiang Province, China, from 2002 to 2015

2017 ◽  
Vol 27 (12) ◽  
pp. 2221-2227 ◽  
Author(s):  
Yin Chen ◽  
Yi Sun ◽  
Juying Yan ◽  
Ziping Miao ◽  
Changping Xu ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Zhejiang Province ◽  
Echovirus 30

Detection of non-polio enteroviruses in Hungary 2000–2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30

Virus Genes ◽  
2010 ◽  
Vol 40 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Beatrix Kapusinszky ◽  
Katalin N. Szomor ◽  
Ágnes Farkas ◽  
Mária Takács ◽  
György Berencsi
Keyword(s):  
Molecular Epidemiology ◽  
Enterovirus 71 ◽  
Coxsackievirus A16 ◽  
Echovirus 30

Molecular epidemiology of Echovirus 30 in Fujian, China between 2001 and 2011

2013 ◽  
Vol 85 (4) ◽  
pp. 696-702 ◽  
Author(s):  
Xiu-hui Yang ◽  
Yan-sheng Yan ◽  
Yu-wei Weng ◽  
Ai-hua He ◽  
Hong-rong Zhang ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Echovirus 30

scholarly journals Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages

2002 ◽  
Vol 76 (10) ◽  
pp. 4940-4949 ◽  
Author(s):  
G. Palacios ◽  
I. Casas ◽  
D. Cisterna ◽  
G. Trallero ◽  
A. Tenorio ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Epidemiology ◽  
Phylogenetic Trees ◽  
Immune Escape ◽  
Amino Acid Sequences ◽  
Clinical Samples ◽  
Genetic Classification ◽  
Echovirus 30 ◽  
Complete Study ◽  
Complete Set

ABSTRACT Echovirus 30 (EV30) is one of the most frequently isolated EVs, causing extensive outbreaks of EV30 aseptic meningitis in temperate climates. EV30 is antigenically heterogeneous, and three major antigenic groups have been defined, although the basis for the antigenic differences is unknown. A reverse transcription-nested PCR which amplifies the 3′-terminal region of the VP1 gene directly from clinical samples was selected for studying EV30 molecular epidemiology, since the major antigenic sites in this region reflect the serotypic pattern of this virus. The different previous approaches to the genetic classification of EV30 were analyzed. A complete study of the EV30 strains was performed by analyzing the sequences from the 112 EV30 strains amplified in this work and the complete set of EV30 strains previously published. A total of 318 strains of EV30 were divided into two broad genotypes (I and II). This classification was supported by the phylogenetic trees obtained from amino acid sequences, and it correlated with the antigenic heterogeneity of the reference strains described in earlier studies. The genotypes could be further divided into subgroups, and these subgroups could be divided into lineages based on their nucleotide distances and levels of bootstrapping. On the other hand, the subgroups and lineages did not result in the same correlation between amino acid and nucleotide differentiation. The molecular epidemiology of EV30 can be compared to influenza virus epidemiology, where prevailing lineages displace the less established lineages on the basis of immune escape. This pattern of evolution is clearly different from that of other enteroviruses. A single lineage at a time appears to be circulating worldwide. This behavior may be related to the epidemic activity of EV30.

scholarly journals Molecular Epidemiology, Diagnostics and Mechanisms of Antibiotic Resistance in Mycobacterium tuberculosis complex in Africa: A Systematic Review of Current Reports

2019 ◽  
Author(s):  
John Osei Sekyere ◽  
Melese Abate Reta ◽  
Nontuthuko Excellent Maningi ◽  
Petrus Bernard Fourie
Keyword(s):  
Systematic Review ◽  
Mycobacterium Tuberculosis ◽  
Molecular Epidemiology ◽  
Public Health Problem ◽  
Amplicon Sequencing ◽  
Global Public Health ◽  
Resistance Mutations ◽  
Quantitative Synthesis ◽  
Almost All ◽  
Global Public Health Problem

AbstractBackgroundTuberculosis (TB) remains a main global public health problem. However, a systematic review of TB resistance epidemiology in Africa is wanting.MethodsA comprehensive systematic search of PubMed, Web of Science and ScienceDirect for English research articles reporting on the molecular epidemiology of Mycobacterium tuberculosis complex resistance in Africa from January 2007 to December 2018 was undertaken.Results and conclusionQualitative and quantitative synthesis were respectively undertaken with 232 and 186 included articles, representing 32 countries. TB monoresistance rate was highest for isoniazid (59%) and rifampicin (27%), particularly in Zimbabwe (100%), Swaziland (100%), and Sudan (67.9%) whilst multidrug resistance (MDR) rate was substantial in Zimbabwe (100%), Sudan (34.6%), Ivory Coast (24.5%) and Ethiopia (23.9%). Resistance-conferring mutations were commonly found in katG (n=3694), rpoB (n=3591), rrs (n=1272), inhA (n=1065), pncA (n=1063) and embB (n=705) in almost all included countries: S315G/I/N/R/T, V473D/F/G/I, Q471H/Q/R/Y, S303C/L etc. in katG; S531A/F/S/G, H526A/C/D/G, D516A/E/G etc. in rpoB; A1401G, A513C etc. in rrs; C15T, G17A/T, -A16G etc. in inhA; Ins456C, Ins 172G, L172P, C14R, Ins515G etc in pncA. Commonest lineages and families such as T (n=8139), LAM (n=5243), Beijing (n=5471), Cameroon (n=3315), CAS (n=2021), H (n=1773) etc., with the exception of T, were not fairly distributed; Beijing, Cameroon and CAS were prevalent in South Africa (n=4964), Ghana (n=2306), and Ethiopia/Tanzania (n=799/635) respectively. Resistance mutations were not lineage-specific and sputum (96.2%) were mainly used for diagnosing TB resistance using the LPA (38.5%), GeneXpert (17.2%), whole-genome sequencing (12.3%) and PCR/amplicon sequencing (9%/23%). Intercountry spread of strains were limited while intra-country dissemination was common. TB resistance and its diagnosis remain a major threat in Africa, necessitating urgent action to contain this global menace.

Molecular epidemiology of vancomycin-resistant enterococci in Singapore

Pathology ◽  
2009 ◽  
pp. 1-5
Author(s):  
Tse Koh ◽  
Beng Low ◽  
Nicholas Leo ◽  
Li-Yang Hsu ◽  
Raymond Lin ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant
Sign in / Sign up

Export Citation Format

Share Document