Differentiation of neural stem cells influences their chemotactic responses to vascular endothelial growth factor

2011 ◽  
Vol 89 (8) ◽  
pp. 1173-1184 ◽  
Author(s):  
Jing Liu ◽  
Youhua Wei ◽  
Yebing Chen ◽  
Xiaojing Xu ◽  
Huanxiang Zhang
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

scholarly journals Direct Stimulation of Adult Neural Stem Cells In Vitro and Neurogenesis In Vivo by Vascular Endothelial Growth Factor

2006 ◽  
Vol 14 (3) ◽  
pp. 237-248 ◽  
Author(s):  
Anne Schänzer ◽  
Frank-Peter Wachs ◽  
Daniel Wilhelm ◽  
Till Acker ◽  
Christiana Cooper-Kuhn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Vascular Endothelial ◽  
Direct Stimulation ◽  
Endothelial Growth Factor ◽  
Stimulation Of

Expression of vascular endothelial growth factor and its receptors in rat neural stem cells

2003 ◽  
Vol 344 (3) ◽  
pp. 165-168 ◽  
Author(s):  
Martin H. Maurer ◽  
Wolf K.C. Tripps ◽  
Robert E. Feldmann ◽  
Wolfgang Kuschinsky
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

scholarly journals Brain Tumor Tropism of Transplanted Human Neural Stem Cells Is Induced by Vascular Endothelial Growth Factor

Neoplasia ◽  
2005 ◽  
Vol 7 (6) ◽  
pp. 623-630 ◽  
Author(s):  
Nils Ole Schmidt ◽  
Wojciech Przylecki ◽  
Wendy Yang ◽  
Mateo Ziu ◽  
Yang Teng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Brain Tumor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Vascular Endothelial ◽  
Human Neural Stem Cells ◽  
Tumor Tropism ◽  
Endothelial Growth Factor

scholarly journals Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Neus Gomila Pelegri ◽  
Catherine A. Gorrie ◽  
Jerran Santos
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factors ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Platelet Derived Growth Factor ◽  
Vascular Endothelial ◽  
Cord Injury ◽  
Endothelial Growth Factor

Neural stem cells have become the focus of many studies as they have the potential to differentiate into all three neural lineages. This may be utilised to develop new and novel ways to treat neurological conditions such as spinal cord and brain injuries, especially if the stem cells can be modulated in vivo without additional invasive surgical procedures. This research is aimed at investigating the effects of the growth factors vascular endothelial growth factor, platelet-derived growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor/platelet-derived growth factor on hippocampal-derived neural stem cells. Cell growth and differentiation were assessed using immunohistochemistry and glutaminase enzyme assay. Cells were cultured for 14 days and treated with different growth factors at two different concentrations 20 ng/mL and 100 ng/mL. At 2 weeks, cells were fixed, and immunohistochemistry was conducted to determine cellular differentiation using antibodies against GFAP, nestin, OSP, and NF200. The cell medium supernatant was also collected during treatment to determine glutaminase levels secreted by the cells as an indicator of neural differentiation. VEGF/PDGF at 100 ng/mL had the greatest influence on cellular proliferation of HNSC, which also stained positively for nestin, OSP, and NF200. In comparison, HNSC in other treatments had poorer cell health and adhesion. HNSC in all treatment groups displayed some differentiation markers and morphology, but this is most significant in the 100 ng/ml VEGF/PDGF treatment. VEGF/PDGF combination produced the optimal effect on the HNSCs inducing the differentiation pathway exhibiting oligodendrocytic and neuronal markers. This is a promising finding that should be further investigated in the brain and spinal cord injury.

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