Nerve growth factor effects and receptors in cultured human neuroblastoma cell lines

1982 ◽  
Vol 8 (2-3) ◽  
pp. 375-391 ◽  
Author(s):  
Kenneth H. Sonnenfeld ◽  
Douglas N. Ishii
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Nerve Growth ◽  
Neuroblastoma Cell Lines

scholarly journals INSULIN-LIKE GROWTH FACTOR BINDING PROTHINS (IGFBPs) IN CONDITIONED MEDIUM FROM HUMAN NEUROBLASTOMA CELL LINES

1993 ◽  
Vol 33 ◽  
pp. S57-S57
Author(s):  
S Bernardini ◽  
S Cianfarani ◽  
R Massoud ◽  
M Annicchiarico-Petruzelli ◽  
G Federici ◽  
...  
Keyword(s):  
Growth Factor ◽  
Conditioned Medium ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Insulin Like Growth Factor ◽  
Human Neuroblastoma ◽  
Factor Binding ◽  
Neuroblastoma Cell Lines

scholarly journals Effects of epidermal growth factor on the [3H]-thymidine uptake in the SK-N-SH and SH-SY5Y human neuroblastoma cell lines

1997 ◽  
Vol 55 (3A) ◽  
pp. 444-451 ◽  
Author(s):  
Luiz Augusto Casulari Roxo da Motta ◽  
Paola Galli ◽  
Flavio Piva ◽  
Roberto Maggi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Cell Subpopulation ◽  
Human Neuroblastoma Cell ◽  
Thymidine Uptake ◽  
Human Neuroblastoma ◽  
Epidermal Growth ◽  
Neuroblastoma Cell Lines

The studies on the factors that regulate the biology of the neuroblastoma cell lines may offer important information on the development of tissues and organs that derive from the neural crest. In the present paper we study the action of epidermal growth factor (EGF) on two human neuroblastoma cell lines: SK-N-SH which is composed at least of two cellular phenotypes (neuroblastic and melanocytic/glial cells), and its pure neuroblastic subclone SH-SY5Y. The results show that EGF (10 ng/ml) significantly stimulates the incorporation of [3H]-thymidine in the SK-N-SH cells only in the presence of fetal bovine serum (FBS) (control = 58285 ± 9327 cpm; EGF =75523 ± 4457; p<0.05). Such effect is not observed in the presence of a chemical defined medium, that is, in the absence of FBS (control = 100997 ± 4375; EGF = 95268 ± 4683; NS). In the SH-SY5Y cells the EGF does not modify the incorporation of [3H]thymidine either in the presence of 10% of BFS (control = 113838 ± 6978; EGF = 119434 ± 9441; NS) or in its absence (control = 46197 ± 3335; EGF = 44472 ± 3493; NS). The results here reported suggest that: a) EGF may affect the proliferation of cells derived from a primary human neuroblastoma; b) this is evident by the EGF-induced increase of [3H]-thymidine incorporation in SK-N-SH cells; c) it is required the presence of other growth factors, present in the FBS, for the mitogenic action to be accomplished; d) since the pure neuroblastic SH-SY5Y cell line are refractory to the EGF, the effects observed in SK-N-SH cells probably occur on the melanocytic/glial cell subpopulation.

Characterization of Two Neuroblastoma Cell Lines Expressing Recombinant Nerve Growth Factor Receptors

1991 ◽  
Vol 56 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Usha Rani Reddy ◽  
Gita Venkatakrishnan ◽  
Amit K. Roy ◽  
Jie Chen ◽  
Mattie Hardy ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Growth Factor Receptors ◽  
Nerve Growth ◽  
Nerve Growth Factor Receptors ◽  
Neuroblastoma Cell Lines
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