Heterogeneity of human neuroblastoma cell lines in their proliferative responses to basic FGF, NGF, and EGF: Correlation with expression of growth factors and growth factor receptors

1995 ◽  
Vol 40 (6) ◽  
pp. 707-715 ◽  
Author(s):  
T. Janet ◽  
G. Lüdecke ◽  
U. Otten ◽  
Klaus Unsicker
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Growth Factor Receptors ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Basic Fgf ◽  
Neuroblastoma Cell Lines

The role of EGFR trafficking in neuroblastoma

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 20003-20003
Author(s):  
P. E. Zage ◽  
Q. Yan ◽  
L. Zeng ◽  
A. J. Bean
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Growth Factor Receptor ◽  
Growth Factor Receptors ◽  
Human Neuroblastoma ◽  
Degradation Rates ◽  
Neuroblastoma Cell Lines

20003 Background: Signaling through growth factor receptors is important in neuroblastoma pathogenesis. Chromosome 1p36 is commonly deleted in neuroblastoma tumors and is associated with a poor prognosis. UBE4B, a gene in 1p36, has been reported mutated in high- risk neuroblastoma. We have found a direct interaction between UBE4B and hrs, a protein required for epidermal growth factor receptor (EGFR) trafficking, suggesting a link between EGFR trafficking and neuroblastoma pathogenesis. We have analyzed the role of UBE4B in the EGFR pathway in neuroblastoma cell lines. Methods: The expression of UBE4B, hrs and EGFR were analyzed by quantitative Western blot in a panel of 7 human neuroblastoma cell lines (SHEP, SKNAS, SKNSH, KCNR, SY5Y, LA155N, NGP). EGFR degradation rates were determined by examining the kinetics of cellular EGFR depletion following a pulse of ligand. Results: UBE4B levels were lowest in SKNAS and highest in NGP cells. Hrs levels were lowest in SKNSH cells and higher in other cell lines. EGFR levels were lowest in NGP and KCNR and highest in SKNAS cells. UBE4B levels were correlated with known 1p deletions. EGFR degradation rates were slowest in SKNAS cells and therefore correlated with cellular UBE4B levels. The low degradation rates were correlated with high cellular levels of EGFR. Conclusions: Expression levels of UBE4B are correlated in neuroblastoma cell lines with chromosome 1p deletions. Cell lines with lower levels of UBE4B degrade EGFR at a markedly slower rate, correlated with higher cellular EGFR levels. We hypothesize that UBE4B affects cell growth by interacting with hrs, directing EGFR for degradation. In its absence the ability of a cell to sort growth factor receptors for degradation is inhibited, resulting in growth factor receptor overabundance and uncontrolled cell growth. These results support the testing of EGFR inhibitors in a future phase I trial for children with neuroblastoma. No significant financial relationships to disclose.

scholarly journals INSULIN-LIKE GROWTH FACTOR BINDING PROTHINS (IGFBPs) IN CONDITIONED MEDIUM FROM HUMAN NEUROBLASTOMA CELL LINES

1993 ◽  
Vol 33 ◽  
pp. S57-S57
Author(s):  
S Bernardini ◽  
S Cianfarani ◽  
R Massoud ◽  
M Annicchiarico-Petruzelli ◽  
G Federici ◽  
...  
Keyword(s):  
Growth Factor ◽  
Conditioned Medium ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Insulin Like Growth Factor ◽  
Human Neuroblastoma ◽  
Factor Binding ◽  
Neuroblastoma Cell Lines

scholarly journals Effects of epidermal growth factor on the [3H]-thymidine uptake in the SK-N-SH and SH-SY5Y human neuroblastoma cell lines

1997 ◽  
Vol 55 (3A) ◽  
pp. 444-451 ◽  
Author(s):  
Luiz Augusto Casulari Roxo da Motta ◽  
Paola Galli ◽  
Flavio Piva ◽  
Roberto Maggi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Cell Subpopulation ◽  
Human Neuroblastoma Cell ◽  
Thymidine Uptake ◽  
Human Neuroblastoma ◽  
Epidermal Growth ◽  
Neuroblastoma Cell Lines

The studies on the factors that regulate the biology of the neuroblastoma cell lines may offer important information on the development of tissues and organs that derive from the neural crest. In the present paper we study the action of epidermal growth factor (EGF) on two human neuroblastoma cell lines: SK-N-SH which is composed at least of two cellular phenotypes (neuroblastic and melanocytic/glial cells), and its pure neuroblastic subclone SH-SY5Y. The results show that EGF (10 ng/ml) significantly stimulates the incorporation of [3H]-thymidine in the SK-N-SH cells only in the presence of fetal bovine serum (FBS) (control = 58285 ± 9327 cpm; EGF =75523 ± 4457; p<0.05). Such effect is not observed in the presence of a chemical defined medium, that is, in the absence of FBS (control = 100997 ± 4375; EGF = 95268 ± 4683; NS). In the SH-SY5Y cells the EGF does not modify the incorporation of [3H]thymidine either in the presence of 10% of BFS (control = 113838 ± 6978; EGF = 119434 ± 9441; NS) or in its absence (control = 46197 ± 3335; EGF = 44472 ± 3493; NS). The results here reported suggest that: a) EGF may affect the proliferation of cells derived from a primary human neuroblastoma; b) this is evident by the EGF-induced increase of [3H]-thymidine incorporation in SK-N-SH cells; c) it is required the presence of other growth factors, present in the FBS, for the mitogenic action to be accomplished; d) since the pure neuroblastic SH-SY5Y cell line are refractory to the EGF, the effects observed in SK-N-SH cells probably occur on the melanocytic/glial cell subpopulation.

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