In situ hybridization histochemistry for the analysis of gene expression in the endocrine and central nervous system tissues: A 3-year experience

1986 ◽  
Vol 16 (1) ◽  
pp. 183-200 ◽  
Author(s):  
B. Bloch ◽  
T. Popovici ◽  
D. Le Guellec ◽  
E. Normand ◽  
S. Chouham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry

scholarly journals Non-radioactive detection of nerve growth factor receptor (NGFR) mRNA in rat brain using in situ hybridization histochemistry.

1991 ◽  
Vol 39 (2) ◽  
pp. 231-234 ◽  
Author(s):  
J E Springer ◽  
E Robbins ◽  
B J Gwag ◽  
M E Lewis ◽  
F Baldino
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Basal Forebrain ◽  
Growth Factor Receptor ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
The Central Nervous System

Radioactively labeled RNA probes in conjunction with in situ hybridization histochemistry have become a useful method for studying gene expression in the central nervous system. We used digoxigenin-labeled uridine triphosphate to synthesize cRNA probes for localization of nerve growth factor receptor (NGFR) mRNA in the rat basal forebrain. Detection of cells containing digoxigenin-labeled NGFR mRNA was accomplished using a digoxigenin antibody conjugated with alkaline phosphatase. NGFR mRNA-positive cells were distributed in three major cell groups in the basal forebrain: the medial septal nucleus, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. This technique provides a rapid and sensitive method for high-resolution detection of mRNA species in the central nervous system, as well as the potential for co-localization of two different mRNA species within individual cells.

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