Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro

1990 ◽  
Vol 25 (4) ◽  
pp. 463-475 ◽  
Author(s):  
M. Murphy ◽  
J. Drago ◽  
P. F. Bartlett
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Fibroblast Growth ◽  
Proliferation And Differentiation

scholarly journals Fibroblast growth factor 2 up regulates telomerase activity in neural precursor cells

Oncogene ◽  
2000 ◽  
Vol 19 (26) ◽  
pp. 2957-2966 ◽  
Author(s):  
Stéphane Haïk ◽  
Laurent R Gauthier ◽  
Christine Granotier ◽  
Jean-Michel Peyrin ◽  
Céline Silva Lages ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Telomerase Activity ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Fibroblast Growth Factor 2 ◽  
Neural Precursor ◽  
Fibroblast Growth

scholarly journals The p107/E2F Pathway Regulates Fibroblast Growth Factor 2 Responsiveness in Neural Precursor Cells

2009 ◽  
Vol 29 (17) ◽  
pp. 4701-4713 ◽  
Author(s):  
Kelly A. McClellan ◽  
Jacqueline L. Vanderluit ◽  
Lisa M. Julian ◽  
Matthew G. Andrusiak ◽  
Delphie Dugal-Tessier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Neural Progenitor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Fibroblast Growth Factor 2 ◽  
Neural Precursor ◽  
Fibroblast Growth

ABSTRACT We have previously shown that p107, a member of the retinoblastoma (Rb) cell cycle regulatory family, has a unique function in regulating the pool of neural precursor cells. As the pool of progenitors is regulated by a limiting supply of trophic factors, we asked if the Rb/E2F pathway may control the size of the progenitor population by regulating the levels of growth factors or their receptors. Here, we demonstrate that fibroblast growth factor 2 (FGF2) is aberrantly upregulated in the brains of animals lacking Rb family proteins and that the gene encoding the FGF2 ligand is directly regulated by p107 and E2F3. Chromatin immunoprecipitation assays demonstrated that E2F3 and p107 occupy E2F consensus sites on the FGF2 promoter in the context of native chromatin. To evaluate the physiological consequence of FGF2 deregulation in both p107 and E2F3 mutants, we measured neural progenitor responsiveness to growth factors. Our results demonstrate that E2F3 and p107 are each mediators of FGF2 growth factor responsiveness in neural progenitor cells. These results support a model whereby p107 regulates the pool of FGF-responsive progenitors by directly regulating FGF2 gene expression in vivo. By identifying novel roles for p107/E2F in regulating genes outside of the classical cell cycle machinery targets, we uncover a new mechanism whereby Rb/E2F mediates proliferation through regulating growth factor responsiveness.

scholarly journals Heparan Sulfates Mediate the Binding of Basic Fibroblast Growth Factor to a Specific Receptor on Neural Precursor Cells

1995 ◽  
Vol 270 (42) ◽  
pp. 24941-24948 ◽  
Author(s):  
Yardenah G. Brickman ◽  
Miriam D. Ford ◽  
David H. Small ◽  
Perry F. Bartlett ◽  
Victor Nurcombe
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Fibroblast Growth ◽  
Specific Receptor ◽  
Heparan Sulfates

scholarly journals Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Pia S. Jensen ◽  
Lise Lyck ◽  
Pia Jensen ◽  
Jens Zimmer ◽  
Morten Meyer
Keyword(s):  
Growth Factor ◽  
Dopaminergic Neurons ◽  
Neural Tissue ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Large Animal ◽  
Ventral Mesencephalic

The potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like Parkinson’s disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons. One current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-termin vitropropagation of the cells. The domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resemblance of the larger gyrencephalic pig brain to the human brain than the commonly used brains of smaller rodents. In the present study, porcine embryonic (28–30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. For passaging, the tissue spheres were cut into quarters, avoiding mechanical or enzymatic dissociation in order to minimize cellular trauma and preserve intercellular contacts. Spheres were propagated for up to 237 days with analysis of cellular content and differentiation at various time points. Our study provides the first demonstration that porcine ventral mesencephalic precursor cells can be long-term propagated as neural tissue spheres, thereby providing an experimental 3Din vitromodel for studies of neural precursor cells, their niche, and differentiation capacity.

Mouse epidermal growth factor-responsive neural precursor cells increase the survival and functional capacity of embryonic rat dopamine neurons in vitro

Neuroreport ◽  
1999 ◽  
Vol 10 (9) ◽  
pp. 1985-1992 ◽  
Author(s):  
Thor Ostenfeld ◽  
Per Horn ◽  
Celine Aardal ◽  
Ingvild Ørpen ◽  
Maeve A. Caldwell ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Functional Capacity ◽  
Precursor Cells ◽  
Dopamine Neurons ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Epidermal Growth
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