Effect of culture duration on chondrogenic preconditioning of equine bone marrow mesenchymal stem cells in self‐assembling peptide hydrogel

2018 ◽  
Vol 37 (6) ◽  
pp. 1368-1375 ◽  
Author(s):  
John D. Kisiday ◽  
Aimee C. Colbath ◽  
Suwimol Tangtrongsup
2016 ◽  
Vol 47 ◽  
pp. 47-54 ◽  
Author(s):  
María José Luesma ◽  
Irene Cantarero ◽  
Beatriz Ranera ◽  
Ana Rosa Remacha ◽  
Tomás Castiella ◽  
...  

2014 ◽  
Vol 22 ◽  
pp. S489
Author(s):  
T. Branly ◽  
M. Hervieu ◽  
S. Jacquet ◽  
L. Bertoni ◽  
T. Gomez-Leduc ◽  
...  

2014 ◽  
Vol 998-999 ◽  
pp. 238-242 ◽  
Author(s):  
Yan Sheng Liao ◽  
Li Deng ◽  
Xiao Qing Gao ◽  
Chao Xian Yang

Objective: To establish composite culture system of rat bone marrow mesenchymal stem cells (BMSCs) and self-assembling peptide hydrogel RADA16-І, and to investigate the effect of RADA16-І hydrogel on neural differentiation of BMSCs. Methods: BMSCs were isolated, cultivated and labeled with green fluorescent protein (GFP), then they were inoculated on glass coverslips or in RADA16-І solution to form control group and RADA16-І group respectively. The morphological changes of BMSCs induced by neural induction medium were observed, and GFAP, NeuN and Map-2 expressions of BMSCs in each group were detected with immunofluorescence. Results: The induced BMSCs presented neuron-like change, and the rates of GFAP and NF-200 positive cells in RADA16-І group were higher than that in control group (P < 0.05). Conclusion: Self-assembling peptide RADA16-І hydrogel can promote neural differentiation of BMSCs, and which may be used as scaffold material on BMSCs transplantation for treatment of nervous system diseases.


2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  

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