Spatial distribution of mineralized bone matrix produced by marrow mesenchymal stem cells in self-assembling peptide hydrogel scaffold

2008 ◽  
Vol 84A (1) ◽  
pp. 128-136 ◽  
Author(s):  
Kazunori Hamada ◽  
Motohiro Hirose ◽  
Toshihiko Yamashita ◽  
Hajime Ohgushi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spatial Distribution ◽  
Bone Matrix ◽  
Hydrogel Scaffold ◽  
Self Assembling ◽  
Marrow Mesenchymal Stem Cells ◽  
Mineralized Bone Matrix ◽  
Peptide Hydrogel

Effects of Self-Assembling Peptide RADA16-І Hydrogel on Neural Differentiation of Bone Marrow Mesenchymal Stem Cells

2014 ◽  
Vol 998-999 ◽  
pp. 238-242 ◽  
Author(s):  
Yan Sheng Liao ◽  
Li Deng ◽  
Xiao Qing Gao ◽  
Chao Xian Yang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neural Differentiation ◽  
Fluorescent Protein ◽  
Morphological Changes ◽  
Control Group ◽  
Induction Medium ◽  
Self Assembling ◽  
Marrow Mesenchymal Stem Cells

Objective: To establish composite culture system of rat bone marrow mesenchymal stem cells (BMSCs) and self-assembling peptide hydrogel RADA16-І, and to investigate the effect of RADA16-І hydrogel on neural differentiation of BMSCs. Methods: BMSCs were isolated, cultivated and labeled with green fluorescent protein (GFP), then they were inoculated on glass coverslips or in RADA16-І solution to form control group and RADA16-І group respectively. The morphological changes of BMSCs induced by neural induction medium were observed, and GFAP, NeuN and Map-2 expressions of BMSCs in each group were detected with immunofluorescence. Results: The induced BMSCs presented neuron-like change, and the rates of GFAP and NF-200 positive cells in RADA16-І group were higher than that in control group (P < 0.05). Conclusion: Self-assembling peptide RADA16-І hydrogel can promote neural differentiation of BMSCs, and which may be used as scaffold material on BMSCs transplantation for treatment of nervous system diseases.

Osteogenic Differentiation of Marrow Mesenchymal Stem Cells (MSCs) on Ceramic Total Joints - Significance of Patient's Serum for Proliferation and Differentiation of MSCs -

2006 ◽  
Vol 309-311 ◽  
pp. 1383-1386
Author(s):  
Hajime Ohgushi ◽  
Hiroko Machida ◽  
Akira Oshima ◽  
Noriko Kotobuki ◽  
Motohiro Hirose ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Marrow Cells ◽  
Bone Matrix ◽  
Old Patients ◽  
Proliferation And Differentiation ◽  
Tissue Engineering Approach ◽  
Marrow Mesenchymal Stem Cells

After culture expansion of mesenchymal stem cells (MSCs) from a few milliliter of fresh patient’s bone marrow, we applied the MSCs on alumina ceramic ankle prosthesis and further cultured in an osteogenic medium for 2 weeks. After the culture, the MSCs differentiated into osteoblasts, which fabricated bone matrix on the surface of ceramic prosthesis. The expansion of MSCs followed by osteogenic differentiation was done using the commercially available medium with some chemicals and patient’s own serum. The MSCs well proliferated and differentiated into osteoblasts, even the MSCs were from old aged (more than 70 years old) patients. The tissue engineered ceramic prostheses were implanted into osteoarthritic patients. Typical X-ray findings showed that radiodense areas began to appear around the cell-seeded areas on the prosthesis about 2 to 3 months after the operation. These findings confirmed the importance of tissue engineering approach for early bone fixation and the approach can be done using small number of bone marrow cells and patient’s own serum without adding animal-derived products.

scholarly journals Enhanced Osteogenesis of Human Mesenchymal Stem Cells by Periodic Heat Shock in Self-Assembling Peptide Hydrogel

2013 ◽  
Vol 19 (5-6) ◽  
pp. 716-728 ◽  
Author(s):  
Jing Chen ◽  
Zhong-Dong Shi ◽  
Xinying Ji ◽  
Jorge Morales ◽  
Jingwei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Human Mesenchymal Stem Cells ◽  
Self Assembling ◽  
Periodic Heat ◽  
Peptide Hydrogel
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